Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23115 and BWI76_RS23120 are separated by 10 nucleotides BWI76_RS23120 and BWI76_RS23125 are separated by 14 nucleotides BWI76_RS23125 and BWI76_RS23130 are separated by 15 nucleotides
BWI76_RS23115: BWI76_RS23115 - propanediol dehydratase large subunit, at 4,818,711 to 4,820,375
_RS23115
BWI76_RS23120: BWI76_RS23120 - propanediol dehydratase medium subunit, at 4,820,386 to 4,821,045
_RS23120
BWI76_RS23125: BWI76_RS23125 - propanediol dehydratase small subunit, at 4,821,060 to 4,821,581
_RS23125
BWI76_RS23130: BWI76_RS23130 - diol dehydratase reactivase subunit alpha, at 4,821,597 to 4,823,429
_RS23130
Position (kb) 4821
4822 Strain fitness (log2 ratio) -1
0
1
2
3
4
5
6 at 4820.086 kb on - strand, within BWI76_RS23115 at 4820.093 kb on + strand, within BWI76_RS23115 at 4820.174 kb on - strand, within BWI76_RS23115 at 4820.192 kb on + strand, within BWI76_RS23115 at 4820.222 kb on - strand at 4820.350 kb on + strand at 4820.352 kb on + strand at 4820.458 kb on - strand, within BWI76_RS23120 at 4820.851 kb on + strand, within BWI76_RS23120 at 4820.920 kb on + strand, within BWI76_RS23120 at 4821.096 kb on + strand at 4821.290 kb on + strand, within BWI76_RS23125 at 4821.448 kb on - strand, within BWI76_RS23125 at 4821.582 kb on + strand at 4821.919 kb on + strand, within BWI76_RS23130 at 4821.951 kb on - strand, within BWI76_RS23130 at 4822.237 kb on - strand, within BWI76_RS23130 at 4822.237 kb on - strand, within BWI76_RS23130 at 4822.242 kb on + strand, within BWI76_RS23130 at 4822.466 kb on - strand, within BWI76_RS23130 at 4822.533 kb on + strand, within BWI76_RS23130 at 4822.538 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 4,820,086 - BWI76_RS23115 0.83 +2.3 4,820,093 + BWI76_RS23115 0.83 +0.2 4,820,174 - BWI76_RS23115 0.88 -0.4 4,820,192 + BWI76_RS23115 0.89 +1.4 4,820,222 - -0.3 4,820,350 + -0.1 4,820,352 + -0.3 4,820,458 - BWI76_RS23120 0.11 +0.1 4,820,851 + BWI76_RS23120 0.70 +1.6 4,820,920 + BWI76_RS23120 0.81 -0.2 4,821,096 + +0.6 4,821,290 + BWI76_RS23125 0.44 -1.3 4,821,448 - BWI76_RS23125 0.74 +5.9 4,821,582 + -1.0 4,821,919 + BWI76_RS23130 0.18 -1.5 4,821,951 - BWI76_RS23130 0.19 -1.2 4,822,237 - BWI76_RS23130 0.35 +0.6 4,822,237 - BWI76_RS23130 0.35 +1.4 4,822,242 + BWI76_RS23130 0.35 -1.0 4,822,466 - BWI76_RS23130 0.47 -0.5 4,822,533 + BWI76_RS23130 0.51 -0.5 4,822,538 + -0.4
Or see this region's nucleotide sequence