Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS13110

Experiment: Parafilmed volatile agar plate with no fungus

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS13100 and BWI76_RS13105 are separated by 12 nucleotidesBWI76_RS13105 and BWI76_RS13110 are separated by 7 nucleotidesBWI76_RS13110 and BWI76_RS13115 overlap by 4 nucleotidesBWI76_RS13115 and BWI76_RS13120 are separated by 2 nucleotides BWI76_RS13100: BWI76_RS13100 - phenylacetate-CoA oxygenase subunit PaaI, at 2,733,026 to 2,733,781 _RS13100 BWI76_RS13105: BWI76_RS13105 - phenylacetate-CoA oxygenase subunit PaaJ, at 2,733,794 to 2,734,291 _RS13105 BWI76_RS13110: BWI76_RS13110 - phenylacetic acid degradation protein, at 2,734,299 to 2,735,369 _RS13110 BWI76_RS13115: BWI76_RS13115 - 2,3-dehydroadipyl-CoA hydratase, at 2,735,366 to 2,736,133 _RS13115 BWI76_RS13120: BWI76_RS13120 - 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase, at 2,736,136 to 2,736,924 _RS13120 Position (kb) 2734 2735 2736Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5at 2733.362 kb on + strand, within BWI76_RS13100at 2733.402 kb on + strand, within BWI76_RS13100at 2733.453 kb on + strand, within BWI76_RS13100at 2733.541 kb on + strand, within BWI76_RS13100at 2733.580 kb on + strand, within BWI76_RS13100at 2733.580 kb on + strand, within BWI76_RS13100at 2733.765 kb on - strandat 2734.083 kb on + strand, within BWI76_RS13105at 2734.176 kb on - strand, within BWI76_RS13105at 2734.235 kb on + strand, within BWI76_RS13105at 2734.454 kb on + strand, within BWI76_RS13110at 2734.932 kb on - strand, within BWI76_RS13110at 2735.127 kb on - strand, within BWI76_RS13110at 2735.337 kb on + strandat 2735.375 kb on - strandat 2736.150 kb on - strandat 2736.166 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus
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2,733,362 + BWI76_RS13100 0.44 -0.4
2,733,402 + BWI76_RS13100 0.50 +5.1
2,733,453 + BWI76_RS13100 0.56 +2.6
2,733,541 + BWI76_RS13100 0.68 -1.9
2,733,580 + BWI76_RS13100 0.73 +1.1
2,733,580 + BWI76_RS13100 0.73 -0.0
2,733,765 - -1.0
2,734,083 + BWI76_RS13105 0.58 +0.6
2,734,176 - BWI76_RS13105 0.77 +2.5
2,734,235 + BWI76_RS13105 0.89 +0.0
2,734,454 + BWI76_RS13110 0.14 -1.5
2,734,932 - BWI76_RS13110 0.59 -1.1
2,735,127 - BWI76_RS13110 0.77 +0.3
2,735,337 + -1.0
2,735,375 - +0.3
2,736,150 - -0.7
2,736,166 - -0.1

Or see this region's nucleotide sequence