Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS12950

Experiment: Parafilmed volatile agar plate with no fungus

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS12940 and BWI76_RS12945 are separated by 57 nucleotidesBWI76_RS12945 and BWI76_RS12950 are separated by 334 nucleotidesBWI76_RS12950 and BWI76_RS12955 are separated by 23 nucleotides BWI76_RS12940: BWI76_RS12940 - AraC family transcriptional regulator, at 2,696,036 to 2,696,857 _RS12940 BWI76_RS12945: BWI76_RS12945 - AraC family transcriptional regulator, at 2,696,915 to 2,697,310 _RS12945 BWI76_RS12950: BWI76_RS12950 - acriflavin resistance protein AcrA, at 2,697,645 to 2,698,820 _RS12950 BWI76_RS12955: BWI76_RS12955 - multidrug efflux RND transporter permease subunit OqxB, at 2,698,844 to 2,701,996 _RS12955 Position (kb) 2697 2698 2699Strain fitness (log2 ratio) -2 -1 0 1at 2696.777 kb on - strandat 2696.859 kb on - strandat 2696.859 kb on - strandat 2697.835 kb on - strand, within BWI76_RS12950at 2698.088 kb on - strand, within BWI76_RS12950at 2698.297 kb on + strand, within BWI76_RS12950at 2699.211 kb on - strand, within BWI76_RS12955at 2699.420 kb on - strand, within BWI76_RS12955at 2699.643 kb on - strand, within BWI76_RS12955

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus
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2,696,777 - +0.6
2,696,859 - +0.4
2,696,859 - +0.9
2,697,835 - BWI76_RS12950 0.16 -0.8
2,698,088 - BWI76_RS12950 0.38 -0.9
2,698,297 + BWI76_RS12950 0.55 -2.5
2,699,211 - BWI76_RS12955 0.12 -0.5
2,699,420 - BWI76_RS12955 0.18 -0.7
2,699,643 - BWI76_RS12955 0.25 -1.0

Or see this region's nucleotide sequence