Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11605 and BWI76_RS11610 are separated by 70 nucleotides BWI76_RS11610 and BWI76_RS11615 overlap by 45 nucleotides BWI76_RS11615 and BWI76_RS11620 are separated by 29 nucleotides BWI76_RS11620 and BWI76_RS11625 are separated by 86 nucleotides
BWI76_RS11605: BWI76_RS11605 - DNA topoisomerase III, at 2,442,026 to 2,443,981
_RS11605
BWI76_RS11610: BWI76_RS11610 - glutamate dehydrogenase, at 2,444,052 to 2,445,395
_RS11610
BWI76_RS11615: BWI76_RS11615 - hypothetical protein, at 2,445,351 to 2,445,548
_RS11615
BWI76_RS11620: BWI76_RS11620 - pyrimidine (deoxy)nucleoside triphosphate pyrophosphohydrolase, at 2,445,578 to 2,445,988
_RS11620
BWI76_RS11625: BWI76_RS11625 - membrane protein, at 2,446,075 to 2,446,692
_RS11625
Position (kb)
2444
2445
2446 Strain fitness (log2 ratio)
-1
0
1 at 2444.153 kb on - strand at 2444.284 kb on + strand, within BWI76_RS11610 at 2444.439 kb on - strand, within BWI76_RS11610 at 2444.464 kb on - strand, within BWI76_RS11610 at 2445.215 kb on - strand, within BWI76_RS11610 at 2445.384 kb on + strand at 2446.039 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 2,444,153 - -0.3 2,444,284 + BWI76_RS11610 0.17 -1.5 2,444,439 - BWI76_RS11610 0.29 -0.9 2,444,464 - BWI76_RS11610 0.31 -1.5 2,445,215 - BWI76_RS11610 0.87 -0.1 2,445,384 + +0.5 2,446,039 - -0.6
Or see this region's nucleotide sequence