Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09405 and BWI76_RS09410 overlap by 38 nucleotides BWI76_RS09410 and BWI76_RS09415 are separated by 74 nucleotides BWI76_RS09415 and BWI76_RS09420 overlap by 38 nucleotides BWI76_RS09420 and BWI76_RS09425 are separated by 264 nucleotides
BWI76_RS09405: BWI76_RS09405 - hypothetical protein, at 1,972,453 to 1,972,632
_RS09405
BWI76_RS09410: BWI76_RS09410 - reverse transcriptase, at 1,972,595 to 1,973,191
_RS09410
BWI76_RS09415: BWI76_RS09415 - IS3 family transposase, at 1,973,266 to 1,974,377
_RS09415
BWI76_RS09420: BWI76_RS09420 - hypothetical protein, at 1,974,340 to 1,974,579
_RS09420
BWI76_RS09425: BWI76_RS09425 - transporter, at 1,974,844 to 1,976,529
_RS09425
Position (kb)
1973
1974
1975 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1972.293 kb on - strand at 1972.329 kb on + strand at 1972.331 kb on + strand at 1972.434 kb on - strand at 1972.434 kb on - strand at 1972.434 kb on - strand at 1972.441 kb on + strand at 1972.458 kb on - strand at 1972.477 kb on + strand, within BWI76_RS09405 at 1972.477 kb on + strand, within BWI76_RS09405 at 1972.509 kb on + strand, within BWI76_RS09405 at 1972.509 kb on + strand, within BWI76_RS09405 at 1972.534 kb on + strand, within BWI76_RS09405 at 1972.544 kb on + strand, within BWI76_RS09405 at 1972.570 kb on + strand, within BWI76_RS09405 at 1972.638 kb on + strand at 1972.646 kb on + strand at 1972.646 kb on - strand at 1972.657 kb on + strand, within BWI76_RS09410 at 1972.665 kb on + strand, within BWI76_RS09410 at 1972.665 kb on + strand, within BWI76_RS09410 at 1972.673 kb on - strand, within BWI76_RS09410 at 1972.691 kb on + strand, within BWI76_RS09410 at 1972.770 kb on - strand, within BWI76_RS09410 at 1972.881 kb on + strand, within BWI76_RS09410 at 1972.974 kb on + strand, within BWI76_RS09410 at 1972.974 kb on + strand, within BWI76_RS09410 at 1973.007 kb on - strand, within BWI76_RS09410 at 1973.019 kb on + strand, within BWI76_RS09410 at 1973.049 kb on + strand, within BWI76_RS09410 at 1973.107 kb on + strand, within BWI76_RS09410 at 1973.161 kb on + strand at 1973.235 kb on - strand at 1974.411 kb on - strand, within BWI76_RS09420 at 1974.785 kb on + strand at 1974.785 kb on + strand at 1974.793 kb on - strand at 1974.857 kb on + strand at 1974.923 kb on + strand at 1975.074 kb on + strand, within BWI76_RS09425 at 1975.300 kb on - strand, within BWI76_RS09425
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 1,972,293 - -1.3 1,972,329 + -0.7 1,972,331 + +1.2 1,972,434 - -1.2 1,972,434 - +0.0 1,972,434 - -0.1 1,972,441 + -1.0 1,972,458 - -1.5 1,972,477 + BWI76_RS09405 0.13 -0.2 1,972,477 + BWI76_RS09405 0.13 +1.1 1,972,509 + BWI76_RS09405 0.31 -0.2 1,972,509 + BWI76_RS09405 0.31 -1.6 1,972,534 + BWI76_RS09405 0.45 +2.6 1,972,544 + BWI76_RS09405 0.51 -0.0 1,972,570 + BWI76_RS09405 0.65 -0.5 1,972,638 + +2.0 1,972,646 + -0.2 1,972,646 - -3.3 1,972,657 + BWI76_RS09410 0.10 -0.1 1,972,665 + BWI76_RS09410 0.12 -1.4 1,972,665 + BWI76_RS09410 0.12 +0.3 1,972,673 - BWI76_RS09410 0.13 -1.3 1,972,691 + BWI76_RS09410 0.16 +0.2 1,972,770 - BWI76_RS09410 0.29 -0.0 1,972,881 + BWI76_RS09410 0.48 -0.3 1,972,974 + BWI76_RS09410 0.63 -0.3 1,972,974 + BWI76_RS09410 0.63 +0.8 1,973,007 - BWI76_RS09410 0.69 -2.6 1,973,019 + BWI76_RS09410 0.71 -1.9 1,973,049 + BWI76_RS09410 0.76 +0.0 1,973,107 + BWI76_RS09410 0.86 -0.6 1,973,161 + -0.8 1,973,235 - +1.4 1,974,411 - BWI76_RS09420 0.30 -1.1 1,974,785 + +0.4 1,974,785 + -0.1 1,974,793 - +2.1 1,974,857 + +0.6 1,974,923 + +0.3 1,975,074 + BWI76_RS09425 0.14 -0.7 1,975,300 - BWI76_RS09425 0.27 -0.7
Or see this region's nucleotide sequence