Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07965 and BWI76_RS07970 are separated by 71 nucleotides BWI76_RS07970 and BWI76_RS07975 are separated by 43 nucleotides BWI76_RS07975 and BWI76_RS07980 are separated by 195 nucleotides
BWI76_RS07965: BWI76_RS07965 - anaerobic C4-dicarboxylate transporter DcuC, at 1,691,704 to 1,693,077
_RS07965
BWI76_RS07970: BWI76_RS07970 - hypothetical protein, at 1,693,149 to 1,693,329
_RS07970
BWI76_RS07975: BWI76_RS07975 - phospholipid:lipid A palmitoyltransferase, at 1,693,373 to 1,693,942
_RS07975
BWI76_RS07980: BWI76_RS07980 - cold-shock protein, at 1,694,138 to 1,694,347
_RS07980
Position (kb) 1693
1694 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 1692.351 kb on + strand, within BWI76_RS07965 at 1692.662 kb on - strand, within BWI76_RS07965 at 1692.913 kb on - strand, within BWI76_RS07965 at 1692.961 kb on + strand at 1692.961 kb on + strand at 1692.969 kb on - strand at 1693.102 kb on + strand at 1693.217 kb on + strand, within BWI76_RS07970 at 1693.225 kb on - strand, within BWI76_RS07970 at 1693.278 kb on + strand, within BWI76_RS07970 at 1693.278 kb on + strand, within BWI76_RS07970 at 1693.428 kb on - strand at 1693.554 kb on - strand, within BWI76_RS07975 at 1693.554 kb on - strand, within BWI76_RS07975 at 1693.668 kb on - strand, within BWI76_RS07975 at 1693.703 kb on - strand, within BWI76_RS07975 at 1693.729 kb on + strand, within BWI76_RS07975 at 1693.729 kb on + strand, within BWI76_RS07975 at 1693.886 kb on - strand at 1693.917 kb on - strand at 1694.157 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 1,692,351 + BWI76_RS07965 0.47 +0.5 1,692,662 - BWI76_RS07965 0.70 -0.2 1,692,913 - BWI76_RS07965 0.88 -0.6 1,692,961 + +4.0 1,692,961 + +0.4 1,692,969 - -0.5 1,693,102 + -0.8 1,693,217 + BWI76_RS07970 0.38 -1.0 1,693,225 - BWI76_RS07970 0.42 -2.1 1,693,278 + BWI76_RS07970 0.71 -0.0 1,693,278 + BWI76_RS07970 0.71 -2.3 1,693,428 - +0.2 1,693,554 - BWI76_RS07975 0.32 -0.0 1,693,554 - BWI76_RS07975 0.32 +1.0 1,693,668 - BWI76_RS07975 0.52 +1.5 1,693,703 - BWI76_RS07975 0.58 -0.1 1,693,729 + BWI76_RS07975 0.62 -0.1 1,693,729 + BWI76_RS07975 0.62 -0.1 1,693,886 - -0.1 1,693,917 - -0.3 1,694,157 + +0.5
Or see this region's nucleotide sequence