Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03865 and BWI76_RS03870 overlap by 4 nucleotides BWI76_RS03870 and BWI76_RS03875 overlap by 4 nucleotides BWI76_RS03875 and BWI76_RS03880 are separated by 284 nucleotides
BWI76_RS03865: BWI76_RS03865 - 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase, at 799,174 to 800,640
_RS03865
BWI76_RS03870: BWI76_RS03870 - 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase, at 800,637 to 801,401
_RS03870
BWI76_RS03875: BWI76_RS03875 - 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase, at 801,398 to 802,030
_RS03875
BWI76_RS03880: BWI76_RS03880 - homoprotocatechuate degradation operon regulator, HpaR, at 802,315 to 802,752
_RS03880
Position (kb) 800
801
802 Strain fitness (log2 ratio) -2
-1
0
1 at 799.832 kb on + strand, within BWI76_RS03865 at 799.840 kb on - strand, within BWI76_RS03865 at 799.850 kb on - strand, within BWI76_RS03865 at 800.010 kb on + strand, within BWI76_RS03865 at 800.136 kb on - strand, within BWI76_RS03865 at 800.392 kb on - strand, within BWI76_RS03865 at 800.579 kb on - strand at 800.888 kb on - strand, within BWI76_RS03870 at 801.013 kb on + strand, within BWI76_RS03870 at 801.133 kb on - strand, within BWI76_RS03870 at 801.172 kb on - strand, within BWI76_RS03870 at 801.244 kb on - strand, within BWI76_RS03870 at 801.246 kb on + strand, within BWI76_RS03870 at 801.249 kb on - strand, within BWI76_RS03870 at 801.255 kb on - strand, within BWI76_RS03870 at 801.351 kb on + strand at 801.494 kb on + strand, within BWI76_RS03875 at 801.651 kb on + strand, within BWI76_RS03875 at 801.950 kb on - strand, within BWI76_RS03875 at 801.957 kb on + strand, within BWI76_RS03875 at 802.313 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 799,832 + BWI76_RS03865 0.45 +0.0 799,840 - BWI76_RS03865 0.45 -0.9 799,850 - BWI76_RS03865 0.46 +0.8 800,010 + BWI76_RS03865 0.57 -0.3 800,136 - BWI76_RS03865 0.66 -0.1 800,392 - BWI76_RS03865 0.83 -0.6 800,579 - +0.4 800,888 - BWI76_RS03870 0.33 -2.0 801,013 + BWI76_RS03870 0.49 -0.1 801,133 - BWI76_RS03870 0.65 -1.1 801,172 - BWI76_RS03870 0.70 -2.3 801,244 - BWI76_RS03870 0.79 -0.0 801,246 + BWI76_RS03870 0.80 -0.0 801,249 - BWI76_RS03870 0.80 +0.8 801,255 - BWI76_RS03870 0.81 +0.4 801,351 + -0.0 801,494 + BWI76_RS03875 0.15 -1.2 801,651 + BWI76_RS03875 0.40 +0.6 801,950 - BWI76_RS03875 0.87 -0.6 801,957 + BWI76_RS03875 0.88 +0.8 802,313 - +0.0
Or see this region's nucleotide sequence