Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27815 and BWI76_RS27820 are separated by 102 nucleotides BWI76_RS27820 and BWI76_RS27825 are separated by 26 nucleotides BWI76_RS27825 and BWI76_RS27830 are separated by 313 nucleotides BWI76_RS27830 and BWI76_RS27835 are separated by 70 nucleotides BWI76_RS27835 and BWI76_RS27840 are separated by 70 nucleotides
BWI76_RS27815: BWI76_RS27815 - acetolactate synthase catalytic subunit, at 5,770,640 to 5,772,328
_RS27815
BWI76_RS27820: BWI76_RS27820 - ilvB operon leader peptide IvbL, at 5,772,431 to 5,772,526
_RS27820
BWI76_RS27825: BWI76_RS27825 - hypothetical protein, at 5,772,553 to 5,772,795
_RS27825
BWI76_RS27830: BWI76_RS27830 - type I toxin-antitoxin system toxin TisB, at 5,773,109 to 5,773,198
_RS27830
BWI76_RS27835: BWI76_RS27835 - acyltransferase, at 5,773,269 to 5,773,715
_RS27835
BWI76_RS27840: BWI76_RS27840 - putative membrane protein, transport, at 5,773,786 to 5,774,619
_RS27840
Position (kb) 5773
5774 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5772.125 kb on - strand, within BWI76_RS27815 at 5772.248 kb on + strand at 5772.499 kb on + strand, within BWI76_RS27820 at 5772.501 kb on + strand, within BWI76_RS27820 at 5772.519 kb on + strand at 5772.519 kb on - strand at 5772.721 kb on + strand, within BWI76_RS27825 at 5772.729 kb on - strand, within BWI76_RS27825 at 5772.729 kb on - strand, within BWI76_RS27825 at 5772.795 kb on - strand at 5772.884 kb on - strand at 5773.054 kb on - strand at 5773.068 kb on - strand at 5773.130 kb on + strand, within BWI76_RS27830 at 5773.178 kb on + strand, within BWI76_RS27830 at 5773.178 kb on + strand, within BWI76_RS27830 at 5773.224 kb on - strand at 5773.305 kb on - strand at 5773.445 kb on - strand, within BWI76_RS27835 at 5773.488 kb on - strand, within BWI76_RS27835 at 5773.582 kb on - strand, within BWI76_RS27835 at 5773.677 kb on - strand at 5773.817 kb on + strand at 5773.898 kb on + strand, within BWI76_RS27840 at 5773.929 kb on + strand, within BWI76_RS27840 at 5773.951 kb on - strand, within BWI76_RS27840 at 5774.008 kb on - strand, within BWI76_RS27840 at 5774.029 kb on - strand, within BWI76_RS27840 at 5774.184 kb on - strand, within BWI76_RS27840 at 5774.195 kb on + strand, within BWI76_RS27840
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 5,772,125 - BWI76_RS27815 0.88 -1.9 5,772,248 + +0.7 5,772,499 + BWI76_RS27820 0.71 -0.4 5,772,501 + BWI76_RS27820 0.73 +2.3 5,772,519 + -0.1 5,772,519 - +0.4 5,772,721 + BWI76_RS27825 0.69 -2.8 5,772,729 - BWI76_RS27825 0.72 -1.5 5,772,729 - BWI76_RS27825 0.72 -0.1 5,772,795 - +0.7 5,772,884 - -0.1 5,773,054 - +0.4 5,773,068 - +0.2 5,773,130 + BWI76_RS27830 0.23 -1.0 5,773,178 + BWI76_RS27830 0.77 -1.3 5,773,178 + BWI76_RS27830 0.77 -2.3 5,773,224 - -0.5 5,773,305 - +0.5 5,773,445 - BWI76_RS27835 0.39 +0.4 5,773,488 - BWI76_RS27835 0.49 -0.1 5,773,582 - BWI76_RS27835 0.70 -0.5 5,773,677 - +0.5 5,773,817 + -0.6 5,773,898 + BWI76_RS27840 0.13 +0.5 5,773,929 + BWI76_RS27840 0.17 +0.4 5,773,951 - BWI76_RS27840 0.20 -0.8 5,774,008 - BWI76_RS27840 0.27 -0.1 5,774,029 - BWI76_RS27840 0.29 +1.8 5,774,184 - BWI76_RS27840 0.48 -0.6 5,774,195 + BWI76_RS27840 0.49 +0.2
Or see this region's nucleotide sequence