Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27100 and BWI76_RS27105 overlap by 4 nucleotides BWI76_RS27105 and BWI76_RS27110 overlap by 8 nucleotides BWI76_RS27110 and BWI76_RS27115 overlap by 7 nucleotides BWI76_RS27115 and BWI76_RS27120 are separated by 159 nucleotides
BWI76_RS27100: BWI76_RS27100 - carbohydrate kinase, at 5,623,536 to 5,625,041
_RS27100
BWI76_RS27105: BWI76_RS27105 - 3-keto-L-gulonate-6-phosphate decarboxylase, at 5,625,038 to 5,625,700
_RS27105
BWI76_RS27110: BWI76_RS27110 - xylulose 5-phosphate 3-epimerase, at 5,625,693 to 5,626,553
_RS27110
BWI76_RS27115: BWI76_RS27115 - L-ribulose-5-phosphate 4-epimerase, at 5,626,547 to 5,627,245
_RS27115
BWI76_RS27120: BWI76_RS27120 - AraC family transcriptional regulator, at 5,627,405 to 5,628,208
_RS27120
Position (kb) 5625
5626
5627 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5624.872 kb on + strand, within BWI76_RS27100 at 5624.879 kb on - strand, within BWI76_RS27100 at 5624.944 kb on + strand at 5625.000 kb on + strand at 5625.000 kb on + strand at 5625.013 kb on - strand at 5625.019 kb on + strand at 5625.079 kb on + strand at 5625.099 kb on + strand at 5625.349 kb on - strand, within BWI76_RS27105 at 5625.416 kb on - strand, within BWI76_RS27105 at 5625.458 kb on + strand, within BWI76_RS27105 at 5625.475 kb on + strand, within BWI76_RS27105 at 5625.525 kb on + strand, within BWI76_RS27105 at 5625.549 kb on + strand, within BWI76_RS27105 at 5625.557 kb on - strand, within BWI76_RS27105 at 5625.582 kb on - strand, within BWI76_RS27105 at 5625.590 kb on + strand, within BWI76_RS27105 at 5625.619 kb on + strand, within BWI76_RS27105 at 5625.743 kb on + strand at 5625.756 kb on - strand at 5625.852 kb on - strand, within BWI76_RS27110 at 5625.869 kb on - strand, within BWI76_RS27110 at 5625.947 kb on + strand, within BWI76_RS27110 at 5625.991 kb on - strand, within BWI76_RS27110 at 5626.103 kb on - strand, within BWI76_RS27110 at 5626.118 kb on + strand at 5626.258 kb on - strand at 5626.334 kb on + strand, within BWI76_RS27110 at 5626.351 kb on + strand, within BWI76_RS27110 at 5626.351 kb on + strand, within BWI76_RS27110 at 5626.508 kb on + strand at 5626.614 kb on + strand at 5626.614 kb on + strand at 5626.686 kb on + strand, within BWI76_RS27115 at 5626.686 kb on + strand, within BWI76_RS27115 at 5626.686 kb on + strand, within BWI76_RS27115 at 5626.693 kb on + strand, within BWI76_RS27115 at 5626.775 kb on - strand, within BWI76_RS27115 at 5626.891 kb on - strand, within BWI76_RS27115 at 5626.952 kb on - strand, within BWI76_RS27115 at 5626.980 kb on - strand, within BWI76_RS27115 at 5626.994 kb on + strand, within BWI76_RS27115 at 5627.016 kb on + strand, within BWI76_RS27115 at 5627.024 kb on - strand, within BWI76_RS27115 at 5627.024 kb on - strand, within BWI76_RS27115 at 5627.042 kb on + strand, within BWI76_RS27115 at 5627.064 kb on + strand, within BWI76_RS27115 at 5627.178 kb on + strand at 5627.179 kb on - strand at 5627.281 kb on + strand at 5627.292 kb on - strand at 5627.340 kb on + strand at 5627.348 kb on - strand at 5627.378 kb on - strand at 5627.410 kb on - strand at 5627.449 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 5,624,872 + BWI76_RS27100 0.89 +0.1 5,624,879 - BWI76_RS27100 0.89 +1.1 5,624,944 + +1.1 5,625,000 + +1.3 5,625,000 + +2.3 5,625,013 - -0.1 5,625,019 + +0.0 5,625,079 + -2.2 5,625,099 + -0.3 5,625,349 - BWI76_RS27105 0.47 -3.1 5,625,416 - BWI76_RS27105 0.57 -0.1 5,625,458 + BWI76_RS27105 0.63 +0.2 5,625,475 + BWI76_RS27105 0.66 +2.8 5,625,525 + BWI76_RS27105 0.73 -0.7 5,625,549 + BWI76_RS27105 0.77 +0.5 5,625,557 - BWI76_RS27105 0.78 -0.1 5,625,582 - BWI76_RS27105 0.82 -0.0 5,625,590 + BWI76_RS27105 0.83 +0.2 5,625,619 + BWI76_RS27105 0.88 -0.1 5,625,743 + -0.7 5,625,756 - -0.4 5,625,852 - BWI76_RS27110 0.18 -0.8 5,625,869 - BWI76_RS27110 0.20 +0.9 5,625,947 + BWI76_RS27110 0.30 +0.1 5,625,991 - BWI76_RS27110 0.35 -0.1 5,626,103 - BWI76_RS27110 0.48 +0.4 5,626,118 + +2.2 5,626,258 - +0.9 5,626,334 + BWI76_RS27110 0.74 +0.2 5,626,351 + BWI76_RS27110 0.76 -1.7 5,626,351 + BWI76_RS27110 0.76 +3.0 5,626,508 + +0.1 5,626,614 + +0.2 5,626,614 + -0.5 5,626,686 + BWI76_RS27115 0.20 -1.1 5,626,686 + BWI76_RS27115 0.20 +1.6 5,626,686 + BWI76_RS27115 0.20 +0.2 5,626,693 + BWI76_RS27115 0.21 -1.5 5,626,775 - BWI76_RS27115 0.33 +2.3 5,626,891 - BWI76_RS27115 0.49 +0.1 5,626,952 - BWI76_RS27115 0.58 -1.2 5,626,980 - BWI76_RS27115 0.62 -0.1 5,626,994 + BWI76_RS27115 0.64 -0.6 5,627,016 + BWI76_RS27115 0.67 +1.6 5,627,024 - BWI76_RS27115 0.68 -0.0 5,627,024 - BWI76_RS27115 0.68 -1.0 5,627,042 + BWI76_RS27115 0.71 -3.1 5,627,064 + BWI76_RS27115 0.74 +3.4 5,627,178 + +0.5 5,627,179 - -0.3 5,627,281 + +1.0 5,627,292 - +1.0 5,627,340 + +0.9 5,627,348 - -0.3 5,627,378 - +0.4 5,627,410 - -0.7 5,627,449 - +0.3
Or see this region's nucleotide sequence