Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS19885 and BWI76_RS19890 are separated by 76 nucleotides BWI76_RS19890 and BWI76_RS19895 overlap by 1 nucleotides BWI76_RS19895 and BWI76_RS19900 are separated by 72 nucleotides
BWI76_RS19885: BWI76_RS19885 - multidrug ABC transporter permease/ATP-binding protein, at 4,158,393 to 4,160,036
_RS19885
BWI76_RS19890: BWI76_RS19890 - alpha-ketoglutarate-dependent dioxygenase AlkB, at 4,160,113 to 4,160,763
_RS19890
BWI76_RS19895: BWI76_RS19895 - bifunctional transcriptional regulator/O6-methylguanine-DNA methyltransferase, at 4,160,763 to 4,161,827
_RS19895
BWI76_RS19900: BWI76_RS19900 - FAD:protein FMN transferase, at 4,161,900 to 4,162,952
_RS19900
Position (kb) 4160
4161
4162 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4159.909 kb on + strand at 4159.943 kb on + strand at 4159.951 kb on - strand at 4159.951 kb on - strand at 4159.951 kb on - strand at 4159.963 kb on - strand at 4160.078 kb on - strand at 4160.201 kb on - strand, within BWI76_RS19890 at 4160.349 kb on + strand, within BWI76_RS19890 at 4160.604 kb on - strand, within BWI76_RS19890 at 4160.724 kb on + strand at 4161.169 kb on + strand, within BWI76_RS19895 at 4161.170 kb on - strand, within BWI76_RS19895 at 4161.170 kb on - strand, within BWI76_RS19895 at 4162.324 kb on + strand, within BWI76_RS19900 at 4162.446 kb on + strand, within BWI76_RS19900 at 4162.783 kb on + strand, within BWI76_RS19900 at 4162.783 kb on + strand, within BWI76_RS19900 at 4162.791 kb on - strand, within BWI76_RS19900 at 4162.793 kb on + strand, within BWI76_RS19900
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 4,159,909 + -0.4 4,159,943 + +0.3 4,159,951 - -0.0 4,159,951 - -0.4 4,159,951 - -2.3 4,159,963 - -0.2 4,160,078 - -1.7 4,160,201 - BWI76_RS19890 0.14 -0.9 4,160,349 + BWI76_RS19890 0.36 +0.5 4,160,604 - BWI76_RS19890 0.75 +0.1 4,160,724 + -0.4 4,161,169 + BWI76_RS19895 0.38 -2.1 4,161,170 - BWI76_RS19895 0.38 -0.8 4,161,170 - BWI76_RS19895 0.38 -0.9 4,162,324 + BWI76_RS19900 0.40 +1.8 4,162,446 + BWI76_RS19900 0.52 -0.5 4,162,783 + BWI76_RS19900 0.84 -2.1 4,162,783 + BWI76_RS19900 0.84 +0.4 4,162,791 - BWI76_RS19900 0.85 -0.4 4,162,793 + BWI76_RS19900 0.85 -2.3
Or see this region's nucleotide sequence