Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS19455 and BWI76_RS19460 are separated by 12 nucleotides BWI76_RS19460 and BWI76_RS19465 are separated by 110 nucleotides BWI76_RS19465 and BWI76_RS19470 are separated by 53 nucleotides
BWI76_RS19455: BWI76_RS19455 - hypothetical protein, at 4,065,632 to 4,067,638
_RS19455
BWI76_RS19460: BWI76_RS19460 - lipoprotein, at 4,067,651 to 4,068,112
_RS19460
BWI76_RS19465: BWI76_RS19465 - hypothetical protein, at 4,068,223 to 4,068,690
_RS19465
BWI76_RS19470: BWI76_RS19470 - DNA-binding response regulator, at 4,068,744 to 4,069,463
_RS19470
Position (kb) 4067
4068
4069 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 4066.931 kb on - strand, within BWI76_RS19455 at 4067.141 kb on + strand, within BWI76_RS19455 at 4067.168 kb on + strand, within BWI76_RS19455 at 4067.226 kb on - strand, within BWI76_RS19455 at 4067.320 kb on + strand, within BWI76_RS19455 at 4067.431 kb on + strand, within BWI76_RS19455 at 4067.613 kb on - strand at 4067.766 kb on - strand, within BWI76_RS19460 at 4067.785 kb on - strand, within BWI76_RS19460 at 4067.824 kb on + strand, within BWI76_RS19460 at 4067.911 kb on - strand, within BWI76_RS19460 at 4068.120 kb on - strand at 4068.441 kb on - strand, within BWI76_RS19465 at 4068.528 kb on + strand, within BWI76_RS19465 at 4068.530 kb on - strand, within BWI76_RS19465 at 4068.653 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 4,066,931 - BWI76_RS19455 0.65 -0.4 4,067,141 + BWI76_RS19455 0.75 +4.8 4,067,168 + BWI76_RS19455 0.77 -0.7 4,067,226 - BWI76_RS19455 0.79 -0.8 4,067,320 + BWI76_RS19455 0.84 -0.2 4,067,431 + BWI76_RS19455 0.90 -0.4 4,067,613 - -0.1 4,067,766 - BWI76_RS19460 0.25 -0.4 4,067,785 - BWI76_RS19460 0.29 -0.6 4,067,824 + BWI76_RS19460 0.37 -1.3 4,067,911 - BWI76_RS19460 0.56 -2.3 4,068,120 - +0.1 4,068,441 - BWI76_RS19465 0.47 +0.3 4,068,528 + BWI76_RS19465 0.65 -0.9 4,068,530 - BWI76_RS19465 0.66 -2.4 4,068,653 + -1.3
Or see this region's nucleotide sequence