Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS19125

Experiment: Parafilmed volatile agar plate with no fungus

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS19115 and BWI76_RS19120 are separated by 27 nucleotidesBWI76_RS19120 and BWI76_RS19125 are separated by 90 nucleotidesBWI76_RS19125 and BWI76_RS19130 are separated by 114 nucleotides BWI76_RS19115: BWI76_RS19115 - outer membrane assembly protein AsmA, at 3,973,669 to 3,975,516 _RS19115 BWI76_RS19120: BWI76_RS19120 - dCTP deaminase, at 3,975,544 to 3,976,125 _RS19120 BWI76_RS19125: BWI76_RS19125 - uridine kinase, at 3,976,216 to 3,976,857 _RS19125 BWI76_RS19130: BWI76_RS19130 - 3-methyladenine DNA glycosylase 2, at 3,976,972 to 3,977,820 _RS19130 Position (kb) 3976 3977Strain fitness (log2 ratio) -1 0 1 2at 3975.669 kb on + strand, within BWI76_RS19120at 3976.696 kb on + strand, within BWI76_RS19125at 3976.970 kb on - strandat 3977.038 kb on - strandat 3977.207 kb on - strand, within BWI76_RS19130at 3977.207 kb on - strand, within BWI76_RS19130at 3977.303 kb on - strand, within BWI76_RS19130at 3977.330 kb on + strand, within BWI76_RS19130at 3977.414 kb on - strand, within BWI76_RS19130at 3977.460 kb on - strand, within BWI76_RS19130at 3977.521 kb on - strand, within BWI76_RS19130at 3977.624 kb on - strand, within BWI76_RS19130at 3977.809 kb on - strandat 3977.854 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus
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3,975,669 + BWI76_RS19120 0.21 -1.3
3,976,696 + BWI76_RS19125 0.75 +1.0
3,976,970 - +0.0
3,977,038 - +0.3
3,977,207 - BWI76_RS19130 0.28 +2.4
3,977,207 - BWI76_RS19130 0.28 -1.3
3,977,303 - BWI76_RS19130 0.39 -0.5
3,977,330 + BWI76_RS19130 0.42 +2.0
3,977,414 - BWI76_RS19130 0.52 +0.5
3,977,460 - BWI76_RS19130 0.57 -0.2
3,977,521 - BWI76_RS19130 0.65 -0.4
3,977,624 - BWI76_RS19130 0.77 +2.0
3,977,809 - -0.7
3,977,854 + +0.6

Or see this region's nucleotide sequence