Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18520 and BWI76_RS18525 are separated by 106 nucleotides BWI76_RS18525 and BWI76_RS18530 are separated by 227 nucleotides BWI76_RS18530 and BWI76_RS18535 overlap by 9 nucleotides
BWI76_RS18520: BWI76_RS18520 - hypothetical protein, at 3,843,546 to 3,844,511
_RS18520
BWI76_RS18525: BWI76_RS18525 - phospholipid:lipid A palmitoyltransferase, at 3,844,618 to 3,845,193
_RS18525
BWI76_RS18530: BWI76_RS18530 - hypothetical protein, at 3,845,421 to 3,846,353
_RS18530
BWI76_RS18535: BWI76_RS18535 - alpha/beta hydrolase, at 3,846,345 to 3,847,886
_RS18535
Position (kb)
3845
3846
3847 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3844.544 kb on - strand at 3844.546 kb on + strand at 3844.687 kb on + strand, within BWI76_RS18525 at 3844.880 kb on + strand, within BWI76_RS18525 at 3844.880 kb on + strand, within BWI76_RS18525 at 3844.888 kb on - strand, within BWI76_RS18525 at 3844.888 kb on - strand, within BWI76_RS18525 at 3844.888 kb on - strand, within BWI76_RS18525 at 3844.888 kb on - strand, within BWI76_RS18525 at 3844.898 kb on - strand, within BWI76_RS18525 at 3844.907 kb on + strand, within BWI76_RS18525 at 3844.915 kb on - strand, within BWI76_RS18525 at 3844.923 kb on + strand, within BWI76_RS18525 at 3845.060 kb on - strand, within BWI76_RS18525 at 3845.060 kb on - strand, within BWI76_RS18525 at 3845.086 kb on + strand, within BWI76_RS18525 at 3845.183 kb on - strand at 3845.195 kb on + strand at 3845.203 kb on - strand at 3845.203 kb on - strand at 3845.203 kb on - strand at 3845.203 kb on - strand at 3845.203 kb on - strand at 3845.286 kb on + strand at 3845.287 kb on + strand at 3845.294 kb on - strand at 3845.322 kb on + strand at 3845.322 kb on + strand at 3845.322 kb on + strand at 3845.322 kb on + strand at 3845.322 kb on + strand at 3845.328 kb on - strand at 3845.330 kb on - strand at 3845.330 kb on - strand at 3845.330 kb on - strand at 3845.330 kb on - strand at 3845.330 kb on - strand at 3845.330 kb on - strand at 3845.330 kb on - strand at 3845.541 kb on + strand, within BWI76_RS18530 at 3845.546 kb on + strand, within BWI76_RS18530 at 3845.584 kb on - strand, within BWI76_RS18530 at 3845.608 kb on + strand, within BWI76_RS18530 at 3845.704 kb on + strand, within BWI76_RS18530 at 3845.728 kb on - strand, within BWI76_RS18530 at 3845.886 kb on + strand, within BWI76_RS18530 at 3845.891 kb on + strand, within BWI76_RS18530 at 3845.899 kb on - strand, within BWI76_RS18530 at 3845.964 kb on + strand, within BWI76_RS18530 at 3845.982 kb on + strand, within BWI76_RS18530 at 3846.101 kb on + strand, within BWI76_RS18530 at 3846.194 kb on - strand, within BWI76_RS18530 at 3846.223 kb on + strand, within BWI76_RS18530 at 3846.271 kb on + strand at 3846.477 kb on - strand at 3846.634 kb on + strand, within BWI76_RS18535 at 3846.928 kb on + strand, within BWI76_RS18535
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 3,844,544 - +0.1 3,844,546 + -0.5 3,844,687 + BWI76_RS18525 0.12 -0.2 3,844,880 + BWI76_RS18525 0.45 -0.8 3,844,880 + BWI76_RS18525 0.45 +0.5 3,844,888 - BWI76_RS18525 0.47 -0.7 3,844,888 - BWI76_RS18525 0.47 +0.4 3,844,888 - BWI76_RS18525 0.47 -0.1 3,844,888 - BWI76_RS18525 0.47 -1.9 3,844,898 - BWI76_RS18525 0.49 +0.7 3,844,907 + BWI76_RS18525 0.50 -0.8 3,844,915 - BWI76_RS18525 0.52 -0.4 3,844,923 + BWI76_RS18525 0.53 +0.7 3,845,060 - BWI76_RS18525 0.77 -0.1 3,845,060 - BWI76_RS18525 0.77 +0.2 3,845,086 + BWI76_RS18525 0.81 +0.7 3,845,183 - -0.2 3,845,195 + -1.6 3,845,203 - -2.2 3,845,203 - -0.4 3,845,203 - +0.5 3,845,203 - +0.7 3,845,203 - +0.3 3,845,286 + -1.2 3,845,287 + -0.1 3,845,294 - -0.9 3,845,322 + -0.5 3,845,322 + -1.6 3,845,322 + +0.2 3,845,322 + +0.1 3,845,322 + -0.2 3,845,328 - +1.4 3,845,330 - +1.2 3,845,330 - -1.6 3,845,330 - -1.5 3,845,330 - -1.3 3,845,330 - +3.7 3,845,330 - -1.7 3,845,330 - +0.6 3,845,541 + BWI76_RS18530 0.13 +1.0 3,845,546 + BWI76_RS18530 0.13 +0.0 3,845,584 - BWI76_RS18530 0.17 -0.2 3,845,608 + BWI76_RS18530 0.20 +0.7 3,845,704 + BWI76_RS18530 0.30 +1.9 3,845,728 - BWI76_RS18530 0.33 -0.7 3,845,886 + BWI76_RS18530 0.50 +0.1 3,845,891 + BWI76_RS18530 0.50 +1.3 3,845,899 - BWI76_RS18530 0.51 -0.4 3,845,964 + BWI76_RS18530 0.58 +0.3 3,845,982 + BWI76_RS18530 0.60 +0.2 3,846,101 + BWI76_RS18530 0.73 +3.4 3,846,194 - BWI76_RS18530 0.83 -0.3 3,846,223 + BWI76_RS18530 0.86 -0.3 3,846,271 + -0.0 3,846,477 - +0.4 3,846,634 + BWI76_RS18535 0.19 -0.0 3,846,928 + BWI76_RS18535 0.38 +0.2
Or see this region's nucleotide sequence