Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS16980

Experiment: Parafilmed volatile agar plate with no fungus

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS16970 and BWI76_RS16975 are separated by 101 nucleotidesBWI76_RS16975 and BWI76_RS16980 are separated by 11 nucleotidesBWI76_RS16980 and BWI76_RS16985 overlap by 4 nucleotides BWI76_RS16970: BWI76_RS16970 - murein L,D-transpeptidase, at 3,519,587 to 3,520,570 _RS16970 BWI76_RS16975: BWI76_RS16975 - cysteine desufuration protein SufE, at 3,520,672 to 3,521,088 _RS16975 BWI76_RS16980: BWI76_RS16980 - bifunctional cysteine desulfurase/selenocysteine lyase, at 3,521,100 to 3,522,320 _RS16980 BWI76_RS16985: BWI76_RS16985 - signal peptide peptidase SppA, at 3,522,317 to 3,523,591 _RS16985 Position (kb) 3521 3522 3523Strain fitness (log2 ratio) -1 0 1at 3520.206 kb on + strand, within BWI76_RS16970at 3520.272 kb on - strand, within BWI76_RS16970at 3520.441 kb on - strand, within BWI76_RS16970at 3520.443 kb on + strand, within BWI76_RS16970at 3520.550 kb on + strandat 3520.648 kb on - strandat 3520.953 kb on + strand, within BWI76_RS16975at 3521.043 kb on + strand, within BWI76_RS16975at 3521.051 kb on - strandat 3521.158 kb on + strandat 3521.161 kb on + strandat 3521.169 kb on - strandat 3521.211 kb on - strandat 3521.367 kb on + strand, within BWI76_RS16980at 3521.390 kb on + strand, within BWI76_RS16980at 3521.708 kb on - strand, within BWI76_RS16980at 3522.043 kb on - strand, within BWI76_RS16980at 3522.327 kb on + strandat 3522.647 kb on + strand, within BWI76_RS16985

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus
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3,520,206 + BWI76_RS16970 0.63 -0.2
3,520,272 - BWI76_RS16970 0.70 -0.3
3,520,441 - BWI76_RS16970 0.87 -0.3
3,520,443 + BWI76_RS16970 0.87 +0.6
3,520,550 + +0.9
3,520,648 - +0.0
3,520,953 + BWI76_RS16975 0.67 +0.2
3,521,043 + BWI76_RS16975 0.89 +0.0
3,521,051 - +0.2
3,521,158 + -0.4
3,521,161 + +0.3
3,521,169 - -0.5
3,521,211 - -0.4
3,521,367 + BWI76_RS16980 0.22 -1.3
3,521,390 + BWI76_RS16980 0.24 -1.2
3,521,708 - BWI76_RS16980 0.50 -1.1
3,522,043 - BWI76_RS16980 0.77 -0.9
3,522,327 + -0.2
3,522,647 + BWI76_RS16985 0.26 -0.6

Or see this region's nucleotide sequence