Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS13105

Experiment: Parafilmed volatile agar plate with no fungus

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS13095 and BWI76_RS13100 are separated by 7 nucleotidesBWI76_RS13100 and BWI76_RS13105 are separated by 12 nucleotidesBWI76_RS13105 and BWI76_RS13110 are separated by 7 nucleotides BWI76_RS13095: BWI76_RS13095 - phenylacetate-CoA oxygenase subunit PaaB, at 2,732,731 to 2,733,018 _RS13095 BWI76_RS13100: BWI76_RS13100 - phenylacetate-CoA oxygenase subunit PaaI, at 2,733,026 to 2,733,781 _RS13100 BWI76_RS13105: BWI76_RS13105 - phenylacetate-CoA oxygenase subunit PaaJ, at 2,733,794 to 2,734,291 _RS13105 BWI76_RS13110: BWI76_RS13110 - phenylacetic acid degradation protein, at 2,734,299 to 2,735,369 _RS13110 Position (kb) 2733 2734 2735Strain fitness (log2 ratio) -2 -1 0 1at 2732.879 kb on + strand, within BWI76_RS13095at 2732.982 kb on + strand, within BWI76_RS13095at 2732.990 kb on - strandat 2733.007 kb on + strandat 2733.042 kb on - strandat 2733.362 kb on + strand, within BWI76_RS13100at 2733.402 kb on + strand, within BWI76_RS13100at 2733.453 kb on + strand, within BWI76_RS13100at 2733.541 kb on + strand, within BWI76_RS13100at 2733.580 kb on + strand, within BWI76_RS13100at 2733.580 kb on + strand, within BWI76_RS13100at 2733.765 kb on - strandat 2734.083 kb on + strand, within BWI76_RS13105at 2734.176 kb on - strand, within BWI76_RS13105at 2734.235 kb on + strand, within BWI76_RS13105at 2734.454 kb on + strand, within BWI76_RS13110at 2734.932 kb on - strand, within BWI76_RS13110at 2735.127 kb on - strand, within BWI76_RS13110

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus
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2,732,879 + BWI76_RS13095 0.51 +0.3
2,732,982 + BWI76_RS13095 0.87 -1.8
2,732,990 - -1.1
2,733,007 + +0.8
2,733,042 - -0.4
2,733,362 + BWI76_RS13100 0.44 +0.3
2,733,402 + BWI76_RS13100 0.50 -0.7
2,733,453 + BWI76_RS13100 0.56 -1.3
2,733,541 + BWI76_RS13100 0.68 -0.2
2,733,580 + BWI76_RS13100 0.73 -1.0
2,733,580 + BWI76_RS13100 0.73 -0.2
2,733,765 - -0.9
2,734,083 + BWI76_RS13105 0.58 -2.4
2,734,176 - BWI76_RS13105 0.77 -0.8
2,734,235 + BWI76_RS13105 0.89 -2.3
2,734,454 + BWI76_RS13110 0.14 +0.6
2,734,932 - BWI76_RS13110 0.59 -1.0
2,735,127 - BWI76_RS13110 0.77 +0.8

Or see this region's nucleotide sequence