Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12650 and BWI76_RS12655 are separated by 56 nucleotides BWI76_RS12655 and BWI76_RS12660 are separated by 177 nucleotides BWI76_RS12660 and BWI76_RS12665 are separated by 154 nucleotides BWI76_RS12665 and BWI76_RS12670 are separated by 275 nucleotides BWI76_RS12670 and BWI76_RS12675 overlap by 1 nucleotides
BWI76_RS12650: BWI76_RS12650 - membrane protein, at 2,639,709 to 2,640,272
_RS12650
BWI76_RS12655: BWI76_RS12655 - PTS cellobiose transporter subunit IIB, at 2,640,329 to 2,640,631
_RS12655
BWI76_RS12660: BWI76_RS12660 - hypothetical protein, at 2,640,809 to 2,641,000
_RS12660
BWI76_RS12665: BWI76_RS12665 - hypothetical protein, at 2,641,155 to 2,641,349
_RS12665
BWI76_RS12670: BWI76_RS12670 - DNA polymerase V subunit UmuD, at 2,641,625 to 2,642,047
_RS12670
BWI76_RS12675: BWI76_RS12675 - putative UV protection and mutation protein, at 2,642,047 to 2,643,312
_RS12675
Position (kb)
2641
2642 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 2640.187 kb on + strand, within BWI76_RS12650 at 2640.210 kb on + strand, within BWI76_RS12650 at 2640.220 kb on + strand at 2640.226 kb on + strand at 2640.227 kb on + strand at 2640.249 kb on + strand at 2640.280 kb on + strand at 2640.349 kb on - strand at 2640.352 kb on + strand at 2640.360 kb on - strand, within BWI76_RS12655 at 2640.369 kb on - strand, within BWI76_RS12655 at 2640.394 kb on - strand, within BWI76_RS12655 at 2640.410 kb on + strand, within BWI76_RS12655 at 2640.410 kb on + strand, within BWI76_RS12655 at 2640.410 kb on + strand, within BWI76_RS12655 at 2640.410 kb on + strand, within BWI76_RS12655 at 2640.418 kb on + strand, within BWI76_RS12655 at 2640.418 kb on - strand, within BWI76_RS12655 at 2640.418 kb on - strand, within BWI76_RS12655 at 2640.418 kb on - strand, within BWI76_RS12655 at 2640.418 kb on - strand, within BWI76_RS12655 at 2640.418 kb on - strand, within BWI76_RS12655 at 2640.418 kb on - strand, within BWI76_RS12655 at 2640.418 kb on - strand, within BWI76_RS12655 at 2640.418 kb on - strand, within BWI76_RS12655 at 2640.418 kb on - strand, within BWI76_RS12655 at 2640.418 kb on - strand at 2640.418 kb on - strand, within BWI76_RS12655 at 2640.418 kb on - strand, within BWI76_RS12655 at 2640.418 kb on - strand, within BWI76_RS12655 at 2640.425 kb on - strand, within BWI76_RS12655 at 2640.427 kb on - strand, within BWI76_RS12655 at 2640.447 kb on + strand, within BWI76_RS12655 at 2640.450 kb on - strand at 2640.455 kb on - strand, within BWI76_RS12655 at 2640.455 kb on - strand, within BWI76_RS12655 at 2640.455 kb on - strand, within BWI76_RS12655 at 2640.455 kb on - strand, within BWI76_RS12655 at 2640.455 kb on - strand, within BWI76_RS12655 at 2640.633 kb on + strand at 2640.641 kb on - strand at 2640.641 kb on - strand at 2640.641 kb on - strand at 2640.641 kb on - strand at 2640.661 kb on + strand at 2640.661 kb on + strand at 2640.747 kb on + strand at 2640.781 kb on + strand at 2640.784 kb on + strand at 2640.784 kb on - strand at 2640.834 kb on - strand, within BWI76_RS12660 at 2640.836 kb on - strand, within BWI76_RS12660 at 2640.836 kb on - strand, within BWI76_RS12660 at 2640.851 kb on + strand, within BWI76_RS12660 at 2640.851 kb on + strand, within BWI76_RS12660 at 2640.857 kb on - strand, within BWI76_RS12660 at 2640.857 kb on - strand, within BWI76_RS12660 at 2640.857 kb on - strand, within BWI76_RS12660 at 2640.858 kb on - strand, within BWI76_RS12660 at 2640.859 kb on - strand, within BWI76_RS12660 at 2640.883 kb on - strand, within BWI76_RS12660 at 2640.958 kb on - strand, within BWI76_RS12660 at 2640.958 kb on - strand, within BWI76_RS12660 at 2640.978 kb on - strand, within BWI76_RS12660 at 2640.983 kb on + strand at 2640.985 kb on + strand at 2640.992 kb on - strand at 2640.993 kb on + strand at 2640.993 kb on + strand at 2640.993 kb on - strand at 2640.993 kb on - strand at 2640.995 kb on + strand at 2641.036 kb on - strand at 2641.036 kb on - strand at 2641.176 kb on + strand, within BWI76_RS12665 at 2641.197 kb on - strand, within BWI76_RS12665 at 2641.386 kb on - strand at 2641.386 kb on - strand at 2641.388 kb on + strand at 2641.396 kb on + strand at 2641.404 kb on - strand at 2641.423 kb on - strand at 2641.456 kb on + strand at 2641.456 kb on + strand at 2641.464 kb on - strand at 2641.808 kb on - strand, within BWI76_RS12670 at 2641.981 kb on + strand, within BWI76_RS12670 at 2642.053 kb on + strand at 2642.244 kb on + strand, within BWI76_RS12675
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 2,640,187 + BWI76_RS12650 0.85 +1.5 2,640,210 + BWI76_RS12650 0.89 +1.1 2,640,220 + +1.0 2,640,226 + -0.8 2,640,227 + +0.8 2,640,249 + +1.0 2,640,280 + +1.7 2,640,349 - -0.4 2,640,352 + +0.9 2,640,360 - BWI76_RS12655 0.10 -1.2 2,640,369 - BWI76_RS12655 0.13 -0.2 2,640,394 - BWI76_RS12655 0.21 +0.2 2,640,410 + BWI76_RS12655 0.27 +0.2 2,640,410 + BWI76_RS12655 0.27 -3.6 2,640,410 + BWI76_RS12655 0.27 -1.5 2,640,410 + BWI76_RS12655 0.27 -0.2 2,640,418 + BWI76_RS12655 0.29 +0.1 2,640,418 - BWI76_RS12655 0.29 -0.2 2,640,418 - BWI76_RS12655 0.29 -1.3 2,640,418 - BWI76_RS12655 0.29 -2.6 2,640,418 - BWI76_RS12655 0.29 +0.3 2,640,418 - BWI76_RS12655 0.29 +0.1 2,640,418 - BWI76_RS12655 0.29 +1.0 2,640,418 - BWI76_RS12655 0.29 -0.3 2,640,418 - BWI76_RS12655 0.29 -2.8 2,640,418 - BWI76_RS12655 0.29 -0.4 2,640,418 - +1.5 2,640,418 - BWI76_RS12655 0.29 +2.2 2,640,418 - BWI76_RS12655 0.29 +0.6 2,640,418 - BWI76_RS12655 0.29 -1.1 2,640,425 - BWI76_RS12655 0.32 +0.0 2,640,427 - BWI76_RS12655 0.32 -0.0 2,640,447 + BWI76_RS12655 0.39 -1.6 2,640,450 - +1.6 2,640,455 - BWI76_RS12655 0.42 +1.1 2,640,455 - BWI76_RS12655 0.42 -0.2 2,640,455 - BWI76_RS12655 0.42 -1.5 2,640,455 - BWI76_RS12655 0.42 +1.0 2,640,455 - BWI76_RS12655 0.42 +1.1 2,640,633 + -1.0 2,640,641 - +0.6 2,640,641 - -1.5 2,640,641 - +1.5 2,640,641 - +1.1 2,640,661 + -0.4 2,640,661 + -1.5 2,640,747 + -0.2 2,640,781 + +1.3 2,640,784 + +0.7 2,640,784 - -1.4 2,640,834 - BWI76_RS12660 0.13 -0.2 2,640,836 - BWI76_RS12660 0.14 +0.4 2,640,836 - BWI76_RS12660 0.14 +0.1 2,640,851 + BWI76_RS12660 0.22 +0.5 2,640,851 + BWI76_RS12660 0.22 -1.9 2,640,857 - BWI76_RS12660 0.25 +1.8 2,640,857 - BWI76_RS12660 0.25 -0.1 2,640,857 - BWI76_RS12660 0.25 -0.6 2,640,858 - BWI76_RS12660 0.26 +0.2 2,640,859 - BWI76_RS12660 0.26 +0.1 2,640,883 - BWI76_RS12660 0.39 -0.4 2,640,958 - BWI76_RS12660 0.78 +0.8 2,640,958 - BWI76_RS12660 0.78 -1.1 2,640,978 - BWI76_RS12660 0.88 -0.8 2,640,983 + -0.7 2,640,985 + +0.1 2,640,992 - +0.2 2,640,993 + +0.3 2,640,993 + +3.6 2,640,993 - -0.2 2,640,993 - -0.5 2,640,995 + +5.0 2,641,036 - -2.0 2,641,036 - -0.2 2,641,176 + BWI76_RS12665 0.11 -1.4 2,641,197 - BWI76_RS12665 0.22 -2.0 2,641,386 - -0.3 2,641,386 - +0.6 2,641,388 + -0.0 2,641,396 + -0.5 2,641,404 - -0.4 2,641,423 - +0.6 2,641,456 + -0.2 2,641,456 + -0.8 2,641,464 - -0.2 2,641,808 - BWI76_RS12670 0.43 +1.1 2,641,981 + BWI76_RS12670 0.84 -0.6 2,642,053 + -0.1 2,642,244 + BWI76_RS12675 0.16 -0.8
Or see this region's nucleotide sequence