Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09950 and BWI76_RS09955 are separated by 65 nucleotides BWI76_RS09955 and BWI76_RS09960 are separated by 69 nucleotides BWI76_RS09960 and BWI76_RS09965 are separated by 185 nucleotides BWI76_RS09965 and BWI76_RS09970 are separated by 95 nucleotides
BWI76_RS09950: BWI76_RS09950 - ribosome modulation factor, at 2,094,910 to 2,095,077
_RS09950
BWI76_RS09955: BWI76_RS09955 - beta-hydroxydecanoyl-ACP dehydratase, at 2,095,143 to 2,095,661
_RS09955
BWI76_RS09960: BWI76_RS09960 - Lon protease, at 2,095,731 to 2,097,488
_RS09960
BWI76_RS09965: BWI76_RS09965 - macrodomain Ter protein, at 2,097,674 to 2,098,126
_RS09965
BWI76_RS09970: BWI76_RS09970 - porin OmpA, at 2,098,222 to 2,099,292
_RS09970
Position (kb)
2095
2096
2097
2098 Strain fitness (log2 ratio)
-2
-1
0
1 at 2095.070 kb on - strand at 2096.165 kb on - strand, within BWI76_RS09960 at 2096.259 kb on - strand, within BWI76_RS09960 at 2097.051 kb on + strand, within BWI76_RS09960 at 2098.178 kb on - strand at 2098.290 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 2,095,070 - +0.1 2,096,165 - BWI76_RS09960 0.25 -2.7 2,096,259 - BWI76_RS09960 0.30 -0.8 2,097,051 + BWI76_RS09960 0.75 -1.2 2,098,178 - +0.3 2,098,290 - +1.3
Or see this region's nucleotide sequence