Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS03440

Experiment: Parafilmed volatile agar plate with no fungus

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS03435 and BWI76_RS03440 are separated by 52 nucleotidesBWI76_RS03440 and BWI76_RS03445 are separated by 144 nucleotides BWI76_RS03435: BWI76_RS03435 - mandelate racemase/muconate lactonizing protein, at 706,391 to 707,494 _RS03435 BWI76_RS03440: BWI76_RS03440 - MFS transporter, at 707,547 to 708,998 _RS03440 BWI76_RS03445: BWI76_RS03445 - hypothetical protein, at 709,143 to 710,792 _RS03445 Position (kb) 707 708 709Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 706.667 kb on + strand, within BWI76_RS03435at 706.714 kb on + strand, within BWI76_RS03435at 706.740 kb on + strand, within BWI76_RS03435at 707.059 kb on + strand, within BWI76_RS03435at 707.165 kb on - strand, within BWI76_RS03435at 707.277 kb on - strand, within BWI76_RS03435at 707.409 kb on + strandat 707.432 kb on - strandat 707.537 kb on - strandat 707.738 kb on + strand, within BWI76_RS03440at 707.746 kb on - strand, within BWI76_RS03440at 707.756 kb on - strand, within BWI76_RS03440at 707.917 kb on - strand, within BWI76_RS03440at 707.922 kb on + strand, within BWI76_RS03440at 708.190 kb on - strand, within BWI76_RS03440at 708.287 kb on - strand, within BWI76_RS03440at 708.304 kb on + strand, within BWI76_RS03440at 708.517 kb on + strand, within BWI76_RS03440at 708.550 kb on + strand, within BWI76_RS03440at 708.599 kb on + strand, within BWI76_RS03440at 708.599 kb on + strand, within BWI76_RS03440at 708.643 kb on + strand, within BWI76_RS03440at 708.674 kb on - strand, within BWI76_RS03440at 708.748 kb on + strand, within BWI76_RS03440at 708.779 kb on + strand, within BWI76_RS03440at 708.809 kb on + strand, within BWI76_RS03440at 708.908 kb on + strandat 708.913 kb on + strandat 708.921 kb on - strandat 708.924 kb on + strandat 708.924 kb on + strandat 708.924 kb on + strandat 708.924 kb on + strandat 708.924 kb on + strandat 708.924 kb on + strandat 708.924 kb on + strandat 708.924 kb on + strandat 708.924 kb on + strandat 708.924 kb on + strandat 708.931 kb on + strandat 708.932 kb on - strandat 708.932 kb on - strandat 708.932 kb on - strandat 708.932 kb on - strandat 708.932 kb on - strandat 708.932 kb on - strandat 708.932 kb on - strandat 708.932 kb on - strandat 708.932 kb on - strandat 708.936 kb on + strandat 708.940 kb on - strandat 708.941 kb on - strandat 708.960 kb on - strandat 709.005 kb on - strandat 709.005 kb on - strandat 709.019 kb on - strandat 709.021 kb on + strandat 709.021 kb on + strandat 709.029 kb on - strandat 709.040 kb on - strandat 709.042 kb on + strandat 709.042 kb on + strandat 709.045 kb on - strandat 709.045 kb on - strandat 709.052 kb on + strandat 709.055 kb on - strandat 709.055 kb on - strandat 709.216 kb on - strandat 709.226 kb on + strandat 709.230 kb on + strandat 709.236 kb on + strandat 709.244 kb on + strandat 709.249 kb on + strandat 709.249 kb on + strandat 709.249 kb on + strandat 709.257 kb on - strandat 709.267 kb on + strandat 709.275 kb on - strandat 709.306 kb on + strandat 709.319 kb on - strand, within BWI76_RS03445at 709.465 kb on + strand, within BWI76_RS03445at 709.465 kb on + strand, within BWI76_RS03445at 709.473 kb on - strand, within BWI76_RS03445at 709.473 kb on - strand, within BWI76_RS03445at 709.483 kb on - strand, within BWI76_RS03445at 709.707 kb on + strand, within BWI76_RS03445at 709.710 kb on - strand, within BWI76_RS03445at 709.755 kb on - strand, within BWI76_RS03445at 709.945 kb on - strand, within BWI76_RS03445at 709.952 kb on + strand, within BWI76_RS03445

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus
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706,667 + BWI76_RS03435 0.25 -0.0
706,714 + BWI76_RS03435 0.29 -0.4
706,740 + BWI76_RS03435 0.32 -1.2
707,059 + BWI76_RS03435 0.61 +0.4
707,165 - BWI76_RS03435 0.70 -0.8
707,277 - BWI76_RS03435 0.80 +0.1
707,409 + +1.2
707,432 - -2.1
707,537 - -1.1
707,738 + BWI76_RS03440 0.13 -0.8
707,746 - BWI76_RS03440 0.14 -0.3
707,756 - BWI76_RS03440 0.14 -0.1
707,917 - BWI76_RS03440 0.25 -1.7
707,922 + BWI76_RS03440 0.26 -0.3
708,190 - BWI76_RS03440 0.44 -2.5
708,287 - BWI76_RS03440 0.51 +1.2
708,304 + BWI76_RS03440 0.52 +0.6
708,517 + BWI76_RS03440 0.67 -1.4
708,550 + BWI76_RS03440 0.69 -0.4
708,599 + BWI76_RS03440 0.72 +0.0
708,599 + BWI76_RS03440 0.72 -1.5
708,643 + BWI76_RS03440 0.75 +0.4
708,674 - BWI76_RS03440 0.78 -0.7
708,748 + BWI76_RS03440 0.83 -0.0
708,779 + BWI76_RS03440 0.85 +0.9
708,809 + BWI76_RS03440 0.87 -1.9
708,908 + -3.0
708,913 + +1.9
708,921 - +0.4
708,924 + -1.2
708,924 + +0.9
708,924 + +1.9
708,924 + -1.1
708,924 + -0.7
708,924 + -0.1
708,924 + -1.4
708,924 + -1.1
708,924 + +1.2
708,924 + -0.5
708,931 + -0.8
708,932 - +0.5
708,932 - +0.9
708,932 - -1.6
708,932 - -0.5
708,932 - -0.2
708,932 - -1.7
708,932 - +0.1
708,932 - -2.1
708,932 - -0.5
708,936 + -2.5
708,940 - +3.0
708,941 - -0.6
708,960 - -0.1
709,005 - +0.1
709,005 - +0.3
709,019 - +1.2
709,021 + -0.2
709,021 + -0.1
709,029 - +1.1
709,040 - +0.3
709,042 + +1.9
709,042 + -1.0
709,045 - +0.7
709,045 - -2.5
709,052 + -0.2
709,055 - -0.4
709,055 - +2.1
709,216 - -0.1
709,226 + -0.3
709,230 + +1.0
709,236 + +2.3
709,244 + -0.4
709,249 + +0.4
709,249 + -0.5
709,249 + -0.1
709,257 - +1.3
709,267 + +2.3
709,275 - +0.7
709,306 + +2.1
709,319 - BWI76_RS03445 0.11 -0.1
709,465 + BWI76_RS03445 0.20 -0.7
709,465 + BWI76_RS03445 0.20 -3.4
709,473 - BWI76_RS03445 0.20 -0.4
709,473 - BWI76_RS03445 0.20 -3.1
709,483 - BWI76_RS03445 0.21 -0.8
709,707 + BWI76_RS03445 0.34 +0.8
709,710 - BWI76_RS03445 0.34 -1.3
709,755 - BWI76_RS03445 0.37 +3.5
709,945 - BWI76_RS03445 0.49 +0.7
709,952 + BWI76_RS03445 0.49 +0.2

Or see this region's nucleotide sequence