Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03395 and BWI76_RS03400 are separated by 146 nucleotides BWI76_RS03400 and BWI76_RS03405 are separated by 289 nucleotides BWI76_RS03405 and BWI76_RS03410 are separated by 473 nucleotides
BWI76_RS03395: BWI76_RS03395 - glyoxalase family protein, at 699,453 to 699,887
_RS03395
BWI76_RS03400: BWI76_RS03400 - hypothetical protein, at 700,034 to 700,357
_RS03400
BWI76_RS03405: BWI76_RS03405 - DNA/RNA non-specific endonuclease, at 700,647 to 701,351
_RS03405
BWI76_RS03410: BWI76_RS03410 - hypothetical protein, at 701,825 to 702,856
_RS03410
Position (kb) 700
701
702 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 699.675 kb on + strand, within BWI76_RS03395 at 699.682 kb on + strand, within BWI76_RS03395 at 699.687 kb on + strand, within BWI76_RS03395 at 699.690 kb on - strand, within BWI76_RS03395 at 699.695 kb on + strand, within BWI76_RS03395 at 699.717 kb on + strand, within BWI76_RS03395 at 699.747 kb on - strand, within BWI76_RS03395 at 699.747 kb on - strand, within BWI76_RS03395 at 699.770 kb on - strand, within BWI76_RS03395 at 699.770 kb on - strand, within BWI76_RS03395 at 699.774 kb on + strand, within BWI76_RS03395 at 699.838 kb on + strand, within BWI76_RS03395 at 699.846 kb on - strand at 699.850 kb on - strand at 699.850 kb on - strand at 699.850 kb on - strand at 699.850 kb on - strand at 699.880 kb on + strand at 699.884 kb on + strand at 699.892 kb on - strand at 699.926 kb on - strand at 699.935 kb on + strand at 699.952 kb on - strand at 699.961 kb on + strand at 699.966 kb on + strand at 699.969 kb on - strand at 699.969 kb on - strand at 699.971 kb on + strand at 699.971 kb on + strand at 699.971 kb on + strand at 699.974 kb on - strand at 699.974 kb on - strand at 699.982 kb on + strand at 699.982 kb on + strand at 699.998 kb on - strand at 700.026 kb on - strand at 700.031 kb on - strand at 700.032 kb on + strand at 700.032 kb on + strand at 700.040 kb on - strand at 700.040 kb on - strand at 700.040 kb on - strand at 700.058 kb on + strand at 700.073 kb on - strand, within BWI76_RS03400 at 700.107 kb on - strand, within BWI76_RS03400 at 700.107 kb on - strand, within BWI76_RS03400 at 700.112 kb on - strand, within BWI76_RS03400 at 700.132 kb on + strand, within BWI76_RS03400 at 700.153 kb on + strand, within BWI76_RS03400 at 700.158 kb on + strand, within BWI76_RS03400 at 700.166 kb on - strand, within BWI76_RS03400 at 700.168 kb on + strand at 700.171 kb on - strand, within BWI76_RS03400 at 700.186 kb on + strand, within BWI76_RS03400 at 700.186 kb on + strand, within BWI76_RS03400 at 700.194 kb on - strand, within BWI76_RS03400 at 700.194 kb on - strand, within BWI76_RS03400 at 700.194 kb on - strand, within BWI76_RS03400 at 700.205 kb on - strand, within BWI76_RS03400 at 700.213 kb on - strand, within BWI76_RS03400 at 700.227 kb on - strand, within BWI76_RS03400 at 700.227 kb on - strand, within BWI76_RS03400 at 700.235 kb on + strand, within BWI76_RS03400 at 700.259 kb on - strand, within BWI76_RS03400 at 700.263 kb on + strand, within BWI76_RS03400 at 700.269 kb on + strand, within BWI76_RS03400 at 700.277 kb on - strand, within BWI76_RS03400 at 700.283 kb on - strand, within BWI76_RS03400 at 700.283 kb on - strand, within BWI76_RS03400 at 700.292 kb on - strand, within BWI76_RS03400 at 700.315 kb on + strand, within BWI76_RS03400 at 700.323 kb on - strand, within BWI76_RS03400 at 700.323 kb on - strand, within BWI76_RS03400 at 700.333 kb on - strand at 700.347 kb on + strand at 700.347 kb on + strand at 700.347 kb on + strand at 700.347 kb on + strand at 700.352 kb on + strand at 700.355 kb on - strand at 700.355 kb on - strand at 700.355 kb on - strand at 700.355 kb on - strand at 700.355 kb on - strand at 700.360 kb on - strand at 700.377 kb on + strand at 700.390 kb on - strand at 700.395 kb on + strand at 700.395 kb on + strand at 700.397 kb on + strand at 700.414 kb on + strand at 700.414 kb on + strand at 700.414 kb on + strand at 700.422 kb on - strand at 700.422 kb on - strand at 700.422 kb on - strand at 700.422 kb on - strand at 700.422 kb on - strand at 700.422 kb on - strand at 700.422 kb on - strand at 700.422 kb on - strand at 700.422 kb on - strand at 700.422 kb on - strand at 700.422 kb on - strand at 700.424 kb on - strand at 700.432 kb on - strand at 700.437 kb on + strand at 700.438 kb on + strand at 700.445 kb on - strand at 700.446 kb on - strand at 700.446 kb on - strand at 700.472 kb on + strand at 700.503 kb on - strand at 700.535 kb on + strand at 700.593 kb on - strand at 700.595 kb on + strand at 700.628 kb on + strand at 700.629 kb on + strand at 700.635 kb on + strand at 700.636 kb on + strand at 700.636 kb on - strand at 700.641 kb on + strand at 700.644 kb on - strand at 700.656 kb on + strand at 700.657 kb on + strand at 700.659 kb on - strand at 700.664 kb on - strand at 700.671 kb on - strand at 700.675 kb on + strand at 700.695 kb on - strand at 700.701 kb on + strand at 700.709 kb on - strand at 700.709 kb on - strand at 700.716 kb on - strand at 700.718 kb on + strand, within BWI76_RS03405 at 700.718 kb on + strand, within BWI76_RS03405 at 700.718 kb on + strand, within BWI76_RS03405 at 700.718 kb on + strand, within BWI76_RS03405 at 700.722 kb on + strand, within BWI76_RS03405 at 700.726 kb on - strand, within BWI76_RS03405 at 700.726 kb on - strand, within BWI76_RS03405 at 700.736 kb on - strand, within BWI76_RS03405 at 700.749 kb on - strand, within BWI76_RS03405 at 700.763 kb on + strand, within BWI76_RS03405 at 700.771 kb on - strand, within BWI76_RS03405 at 700.844 kb on + strand, within BWI76_RS03405 at 700.887 kb on + strand, within BWI76_RS03405 at 700.900 kb on - strand, within BWI76_RS03405 at 700.929 kb on - strand, within BWI76_RS03405 at 701.005 kb on - strand, within BWI76_RS03405 at 701.042 kb on + strand, within BWI76_RS03405 at 701.086 kb on + strand, within BWI76_RS03405 at 701.212 kb on - strand, within BWI76_RS03405 at 701.212 kb on - strand, within BWI76_RS03405 at 701.224 kb on + strand, within BWI76_RS03405 at 701.237 kb on - strand, within BWI76_RS03405 at 701.237 kb on - strand, within BWI76_RS03405 at 701.242 kb on - strand, within BWI76_RS03405 at 701.272 kb on + strand, within BWI76_RS03405 at 701.280 kb on - strand, within BWI76_RS03405 at 701.297 kb on + strand at 701.298 kb on - strand at 701.305 kb on - strand at 701.306 kb on - strand at 701.308 kb on + strand at 701.318 kb on + strand at 701.326 kb on - strand at 701.349 kb on - strand at 701.359 kb on - strand at 701.375 kb on + strand at 701.375 kb on + strand at 701.383 kb on - strand at 701.383 kb on - strand at 701.384 kb on - strand at 701.384 kb on - strand at 701.411 kb on + strand at 701.430 kb on + strand at 701.435 kb on + strand at 701.438 kb on - strand at 701.461 kb on - strand at 701.461 kb on - strand at 701.461 kb on - strand at 701.463 kb on + strand at 701.471 kb on - strand at 701.484 kb on - strand at 701.507 kb on + strand at 701.529 kb on + strand at 701.537 kb on - strand at 701.537 kb on - strand at 701.537 kb on - strand at 701.605 kb on - strand at 701.620 kb on + strand at 701.636 kb on + strand at 701.640 kb on + strand at 701.645 kb on + strand at 701.646 kb on + strand at 701.665 kb on + strand at 701.688 kb on - strand at 701.690 kb on + strand at 701.693 kb on - strand at 701.704 kb on - strand at 701.706 kb on + strand at 701.706 kb on + strand at 701.706 kb on + strand at 701.706 kb on + strand at 701.706 kb on + strand at 701.706 kb on + strand at 701.706 kb on + strand at 701.706 kb on + strand at 701.706 kb on + strand at 701.711 kb on + strand at 701.711 kb on + strand at 701.713 kb on + strand at 701.713 kb on + strand at 701.713 kb on + strand at 701.713 kb on + strand at 701.713 kb on + strand at 701.713 kb on + strand at 701.713 kb on + strand at 701.713 kb on + strand at 701.713 kb on + strand at 701.713 kb on + strand at 701.713 kb on + strand at 701.713 kb on + strand at 701.713 kb on + strand at 701.713 kb on + strand at 701.714 kb on - strand at 701.714 kb on - strand at 701.714 kb on - strand at 701.714 kb on - strand at 701.714 kb on - strand at 701.714 kb on - strand at 701.714 kb on - strand at 701.714 kb on - strand at 701.721 kb on - strand at 701.721 kb on - strand at 701.721 kb on - strand at 701.721 kb on - strand at 701.721 kb on - strand at 701.721 kb on - strand at 701.721 kb on - strand at 701.721 kb on - strand at 701.721 kb on - strand at 701.724 kb on - strand at 701.732 kb on - strand at 701.732 kb on - strand at 701.758 kb on + strand at 701.764 kb on - strand at 701.766 kb on - strand at 701.772 kb on - strand at 701.782 kb on + strand at 701.782 kb on + strand at 701.792 kb on + strand at 701.792 kb on + strand at 701.799 kb on - strand at 701.802 kb on - strand at 701.839 kb on + strand at 701.845 kb on - strand at 701.860 kb on + strand at 701.860 kb on + strand at 701.861 kb on + strand at 701.865 kb on + strand at 701.868 kb on - strand at 701.965 kb on - strand, within BWI76_RS03410 at 702.300 kb on + strand, within BWI76_RS03410
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 699,675 + BWI76_RS03395 0.51 -1.7 699,682 + BWI76_RS03395 0.53 -1.7 699,687 + BWI76_RS03395 0.54 -0.3 699,690 - BWI76_RS03395 0.54 +1.5 699,695 + BWI76_RS03395 0.56 -1.2 699,717 + BWI76_RS03395 0.61 -0.8 699,747 - BWI76_RS03395 0.68 -1.0 699,747 - BWI76_RS03395 0.68 +0.3 699,770 - BWI76_RS03395 0.73 -1.2 699,770 - BWI76_RS03395 0.73 -0.3 699,774 + BWI76_RS03395 0.74 -2.1 699,838 + BWI76_RS03395 0.89 +0.1 699,846 - -0.5 699,850 - -2.2 699,850 - +0.5 699,850 - -0.3 699,850 - -1.3 699,880 + -1.3 699,884 + -0.4 699,892 - +0.4 699,926 - -1.7 699,935 + -1.8 699,952 - +0.3 699,961 + +0.2 699,966 + +0.8 699,969 - -1.1 699,969 - +2.5 699,971 + +1.4 699,971 + -1.8 699,971 + +0.7 699,974 - -0.4 699,974 - -2.1 699,982 + -2.7 699,982 + -0.4 699,998 - -0.9 700,026 - -1.4 700,031 - -0.2 700,032 + +0.8 700,032 + -0.8 700,040 - +0.8 700,040 - -0.4 700,040 - -0.5 700,058 + +1.6 700,073 - BWI76_RS03400 0.12 -0.1 700,107 - BWI76_RS03400 0.23 -0.0 700,107 - BWI76_RS03400 0.23 -1.2 700,112 - BWI76_RS03400 0.24 +1.0 700,132 + BWI76_RS03400 0.30 -0.7 700,153 + BWI76_RS03400 0.37 -0.8 700,158 + BWI76_RS03400 0.38 -0.7 700,166 - BWI76_RS03400 0.41 -0.9 700,168 + -0.6 700,171 - BWI76_RS03400 0.42 -0.2 700,186 + BWI76_RS03400 0.47 -0.1 700,186 + BWI76_RS03400 0.47 -1.3 700,194 - BWI76_RS03400 0.49 -1.2 700,194 - BWI76_RS03400 0.49 +4.5 700,194 - BWI76_RS03400 0.49 -0.3 700,205 - BWI76_RS03400 0.53 -1.9 700,213 - BWI76_RS03400 0.55 -0.6 700,227 - BWI76_RS03400 0.60 -0.4 700,227 - BWI76_RS03400 0.60 +1.0 700,235 + BWI76_RS03400 0.62 +1.3 700,259 - BWI76_RS03400 0.69 -0.5 700,263 + BWI76_RS03400 0.71 +1.5 700,269 + BWI76_RS03400 0.73 -0.4 700,277 - BWI76_RS03400 0.75 -1.2 700,283 - BWI76_RS03400 0.77 -0.6 700,283 - BWI76_RS03400 0.77 +0.2 700,292 - BWI76_RS03400 0.80 -1.9 700,315 + BWI76_RS03400 0.87 +0.8 700,323 - BWI76_RS03400 0.89 -0.8 700,323 - BWI76_RS03400 0.89 -2.8 700,333 - -0.1 700,347 + +4.4 700,347 + +0.1 700,347 + -0.4 700,347 + +0.2 700,352 + -0.3 700,355 - -0.7 700,355 - +1.4 700,355 - +1.4 700,355 - -0.6 700,355 - -1.8 700,360 - -1.6 700,377 + +0.1 700,390 - +0.6 700,395 + +0.3 700,395 + -1.2 700,397 + +0.3 700,414 + -0.2 700,414 + -0.7 700,414 + -2.9 700,422 - +0.2 700,422 - -0.6 700,422 - -0.0 700,422 - -0.4 700,422 - +0.7 700,422 - -0.5 700,422 - -1.4 700,422 - +0.3 700,422 - -2.0 700,422 - -1.5 700,422 - +1.2 700,424 - -0.4 700,432 - +0.3 700,437 + +0.1 700,438 + -0.6 700,445 - +0.1 700,446 - +0.6 700,446 - -1.3 700,472 + +0.7 700,503 - +0.0 700,535 + +0.4 700,593 - +0.7 700,595 + -2.7 700,628 + -1.0 700,629 + -0.1 700,635 + -1.5 700,636 + -0.6 700,636 - -1.1 700,641 + +0.2 700,644 - +2.4 700,656 + -0.2 700,657 + +0.8 700,659 - -0.2 700,664 - +0.3 700,671 - -1.1 700,675 + +0.8 700,695 - +1.4 700,701 + -1.1 700,709 - -0.9 700,709 - -0.2 700,716 - -0.2 700,718 + BWI76_RS03405 0.10 -0.5 700,718 + BWI76_RS03405 0.10 -1.3 700,718 + BWI76_RS03405 0.10 +1.4 700,718 + BWI76_RS03405 0.10 -1.5 700,722 + BWI76_RS03405 0.11 -0.5 700,726 - BWI76_RS03405 0.11 -0.9 700,726 - BWI76_RS03405 0.11 -1.2 700,736 - BWI76_RS03405 0.13 +0.0 700,749 - BWI76_RS03405 0.14 +1.8 700,763 + BWI76_RS03405 0.16 -0.3 700,771 - BWI76_RS03405 0.18 +0.4 700,844 + BWI76_RS03405 0.28 -0.5 700,887 + BWI76_RS03405 0.34 -0.5 700,900 - BWI76_RS03405 0.36 +0.1 700,929 - BWI76_RS03405 0.40 +0.4 701,005 - BWI76_RS03405 0.51 +1.2 701,042 + BWI76_RS03405 0.56 +0.3 701,086 + BWI76_RS03405 0.62 -1.2 701,212 - BWI76_RS03405 0.80 -0.8 701,212 - BWI76_RS03405 0.80 -1.3 701,224 + BWI76_RS03405 0.82 -0.6 701,237 - BWI76_RS03405 0.84 -0.7 701,237 - BWI76_RS03405 0.84 -0.4 701,242 - BWI76_RS03405 0.84 +0.3 701,272 + BWI76_RS03405 0.89 -0.1 701,280 - BWI76_RS03405 0.90 -0.6 701,297 + -0.2 701,298 - -0.1 701,305 - +1.6 701,306 - +0.1 701,308 + +0.3 701,318 + +0.4 701,326 - -2.5 701,349 - +0.3 701,359 - +0.3 701,375 + +0.3 701,375 + -0.9 701,383 - -1.9 701,383 - -0.1 701,384 - +0.4 701,384 - +0.1 701,411 + +2.6 701,430 + +0.4 701,435 + +1.9 701,438 - +0.8 701,461 - -0.4 701,461 - +1.5 701,461 - -1.7 701,463 + -0.8 701,471 - +0.8 701,484 - +1.5 701,507 + -0.4 701,529 + -3.1 701,537 - -0.2 701,537 - -0.9 701,537 - -1.9 701,605 - -0.4 701,620 + -0.8 701,636 + -0.2 701,640 + +0.3 701,645 + -0.1 701,646 + +2.0 701,665 + +0.6 701,688 - -0.3 701,690 + +0.8 701,693 - +0.5 701,704 - -0.0 701,706 + -2.4 701,706 + -0.1 701,706 + -1.4 701,706 + -1.4 701,706 + -0.2 701,706 + +0.4 701,706 + +0.9 701,706 + +0.3 701,706 + +1.4 701,711 + -0.7 701,711 + -1.7 701,713 + +0.5 701,713 + +0.6 701,713 + -0.1 701,713 + -0.3 701,713 + +0.4 701,713 + +1.0 701,713 + +2.7 701,713 + -0.2 701,713 + -1.1 701,713 + +0.0 701,713 + +4.6 701,713 + -3.2 701,713 + +0.4 701,713 + -0.8 701,714 - -1.3 701,714 - +0.1 701,714 - +0.1 701,714 - -0.2 701,714 - +2.4 701,714 - +2.6 701,714 - -0.0 701,714 - -0.1 701,721 - +0.5 701,721 - -0.6 701,721 - -1.4 701,721 - -1.0 701,721 - +0.1 701,721 - -0.4 701,721 - -0.1 701,721 - +0.8 701,721 - -2.7 701,724 - +1.3 701,732 - -2.3 701,732 - -0.7 701,758 + +0.5 701,764 - -0.1 701,766 - -2.4 701,772 - +0.5 701,782 + +1.0 701,782 + -1.1 701,792 + -1.8 701,792 + +1.1 701,799 - -0.4 701,802 - -0.8 701,839 + +0.7 701,845 - -1.8 701,860 + +0.1 701,860 + -1.8 701,861 + +0.6 701,865 + -1.3 701,868 - -0.0 701,965 - BWI76_RS03410 0.14 -0.2 702,300 + BWI76_RS03410 0.46 +1.0
Or see this region's nucleotide sequence