Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00500 and BWI76_RS00505 are separated by 11 nucleotides BWI76_RS00505 and BWI76_RS00510 are separated by 13 nucleotides BWI76_RS00510 and BWI76_RS00515 overlap by 4 nucleotides BWI76_RS00515 and BWI76_RS00520 are separated by 175 nucleotides
BWI76_RS00500: BWI76_RS00500 - PTS sorbose IIC component, at 102,606 to 103,409
_RS00500
BWI76_RS00505: BWI76_RS00505 - PTS system, sorbose-specific IIB component, at 103,421 to 103,918
_RS00505
BWI76_RS00510: BWI76_RS00510 - glucuronyl hydrolase, at 103,932 to 105,110
_RS00510
BWI76_RS00515: BWI76_RS00515 - PTS fructose transporter subunit IIA, at 105,107 to 105,589
_RS00515
BWI76_RS00520: BWI76_RS00520 - transcription regulator of PTS system, at 105,765 to 108,368
_RS00520
Position (kb) 103
104
105
106 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 102.936 kb on - strand, within BWI76_RS00500 at 102.985 kb on + strand, within BWI76_RS00500 at 103.017 kb on + strand, within BWI76_RS00500 at 103.025 kb on - strand, within BWI76_RS00500 at 103.058 kb on + strand, within BWI76_RS00500 at 103.063 kb on + strand, within BWI76_RS00500 at 103.063 kb on + strand, within BWI76_RS00500 at 103.066 kb on - strand, within BWI76_RS00500 at 103.071 kb on - strand, within BWI76_RS00500 at 103.302 kb on - strand, within BWI76_RS00500 at 103.309 kb on + strand, within BWI76_RS00500 at 103.346 kb on + strand at 103.346 kb on + strand at 103.360 kb on + strand at 103.505 kb on + strand, within BWI76_RS00505 at 103.720 kb on - strand, within BWI76_RS00505 at 103.824 kb on - strand, within BWI76_RS00505 at 103.845 kb on + strand, within BWI76_RS00505 at 103.865 kb on - strand, within BWI76_RS00505 at 103.933 kb on - strand at 103.944 kb on + strand at 103.963 kb on - strand at 103.968 kb on + strand at 104.111 kb on + strand, within BWI76_RS00510 at 104.119 kb on - strand, within BWI76_RS00510 at 104.119 kb on - strand, within BWI76_RS00510 at 104.119 kb on - strand, within BWI76_RS00510 at 104.223 kb on - strand, within BWI76_RS00510 at 104.265 kb on + strand, within BWI76_RS00510 at 104.341 kb on + strand, within BWI76_RS00510 at 104.347 kb on - strand, within BWI76_RS00510 at 104.349 kb on - strand, within BWI76_RS00510 at 104.349 kb on - strand, within BWI76_RS00510 at 104.460 kb on - strand, within BWI76_RS00510 at 104.654 kb on - strand, within BWI76_RS00510 at 104.727 kb on - strand, within BWI76_RS00510 at 104.765 kb on - strand, within BWI76_RS00510 at 104.772 kb on - strand, within BWI76_RS00510 at 104.796 kb on - strand, within BWI76_RS00510 at 104.829 kb on + strand at 105.106 kb on + strand at 105.113 kb on - strand at 105.124 kb on - strand at 105.161 kb on - strand, within BWI76_RS00515 at 105.201 kb on + strand, within BWI76_RS00515 at 105.312 kb on + strand, within BWI76_RS00515 at 105.444 kb on - strand, within BWI76_RS00515 at 105.457 kb on - strand, within BWI76_RS00515 at 105.512 kb on - strand, within BWI76_RS00515 at 105.526 kb on + strand, within BWI76_RS00515 at 105.614 kb on - strand at 105.726 kb on - strand at 105.734 kb on - strand at 105.762 kb on + strand at 105.762 kb on + strand at 105.762 kb on + strand at 105.763 kb on + strand at 105.770 kb on - strand at 105.770 kb on - strand at 105.771 kb on - strand at 105.771 kb on - strand at 105.784 kb on - strand at 105.855 kb on + strand at 105.940 kb on - strand at 106.051 kb on + strand, within BWI76_RS00520
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 102,936 - BWI76_RS00500 0.41 -0.6 102,985 + BWI76_RS00500 0.47 -1.8 103,017 + BWI76_RS00500 0.51 +0.9 103,025 - BWI76_RS00500 0.52 +3.0 103,058 + BWI76_RS00500 0.56 -0.6 103,063 + BWI76_RS00500 0.57 +1.1 103,063 + BWI76_RS00500 0.57 -0.0 103,066 - BWI76_RS00500 0.57 +0.3 103,071 - BWI76_RS00500 0.58 -0.0 103,302 - BWI76_RS00500 0.87 +0.3 103,309 + BWI76_RS00500 0.87 +1.9 103,346 + -1.1 103,346 + +1.3 103,360 + +0.5 103,505 + BWI76_RS00505 0.17 -1.3 103,720 - BWI76_RS00505 0.60 -0.5 103,824 - BWI76_RS00505 0.81 +0.0 103,845 + BWI76_RS00505 0.85 -0.6 103,865 - BWI76_RS00505 0.89 -2.4 103,933 - -0.2 103,944 + +0.9 103,963 - -0.8 103,968 + +0.2 104,111 + BWI76_RS00510 0.15 +0.4 104,119 - BWI76_RS00510 0.16 -0.1 104,119 - BWI76_RS00510 0.16 +0.9 104,119 - BWI76_RS00510 0.16 +1.7 104,223 - BWI76_RS00510 0.25 +0.5 104,265 + BWI76_RS00510 0.28 -1.7 104,341 + BWI76_RS00510 0.35 -0.1 104,347 - BWI76_RS00510 0.35 -1.9 104,349 - BWI76_RS00510 0.35 -1.6 104,349 - BWI76_RS00510 0.35 +0.5 104,460 - BWI76_RS00510 0.45 -3.0 104,654 - BWI76_RS00510 0.61 -0.3 104,727 - BWI76_RS00510 0.67 -1.7 104,765 - BWI76_RS00510 0.71 -2.5 104,772 - BWI76_RS00510 0.71 -0.5 104,796 - BWI76_RS00510 0.73 -0.7 104,829 + +1.5 105,106 + -0.9 105,113 - +1.5 105,124 - -0.0 105,161 - BWI76_RS00515 0.11 -0.1 105,201 + BWI76_RS00515 0.19 +0.3 105,312 + BWI76_RS00515 0.42 -0.1 105,444 - BWI76_RS00515 0.70 +0.6 105,457 - BWI76_RS00515 0.72 -0.5 105,512 - BWI76_RS00515 0.84 -0.1 105,526 + BWI76_RS00515 0.87 -1.2 105,614 - -0.5 105,726 - -0.8 105,734 - -1.5 105,762 + +0.7 105,762 + -1.2 105,762 + -1.4 105,763 + +0.6 105,770 - -0.7 105,770 - +0.2 105,771 - +0.2 105,771 - -2.4 105,784 - +0.3 105,855 + +0.5 105,940 - +0.4 106,051 + BWI76_RS00520 0.11 +0.3
Or see this region's nucleotide sequence