Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00260 and BWI76_RS00265 are separated by 218 nucleotides BWI76_RS00265 and BWI76_RS00270 are separated by 182 nucleotides BWI76_RS00270 and BWI76_RS00275 are separated by 10 nucleotides
BWI76_RS00260: BWI76_RS00260 - ATPase RavA, at 52,540 to 54,039
_RS00260
BWI76_RS00265: BWI76_RS00265 - potassium transporter Kup, at 54,258 to 56,126
_RS00265
BWI76_RS00270: BWI76_RS00270 - D-ribose pyranase (EC 5.4.99.62) <small>(from data)</small>, at 56,309 to 56,728
_RS00270
BWI76_RS00275: BWI76_RS00275 - ribose ABC transporter ATP-binding protein RbsA, at 56,739 to 58,244
_RS00275
Position (kb) 54
55
56
57 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 53.362 kb on + strand, within BWI76_RS00260 at 53.417 kb on - strand, within BWI76_RS00260 at 53.417 kb on - strand, within BWI76_RS00260 at 53.441 kb on + strand, within BWI76_RS00260 at 53.457 kb on - strand, within BWI76_RS00260 at 53.671 kb on - strand, within BWI76_RS00260 at 53.687 kb on + strand, within BWI76_RS00260 at 53.855 kb on - strand, within BWI76_RS00260 at 53.951 kb on - strand at 53.951 kb on - strand at 53.951 kb on - strand at 53.993 kb on + strand at 54.028 kb on + strand at 54.089 kb on - strand at 54.093 kb on + strand at 54.142 kb on + strand at 54.142 kb on + strand at 54.142 kb on + strand at 54.142 kb on + strand at 54.150 kb on - strand at 54.163 kb on + strand at 54.184 kb on + strand at 54.342 kb on + strand at 54.344 kb on + strand at 54.351 kb on - strand at 54.351 kb on - strand at 54.392 kb on + strand at 54.506 kb on - strand, within BWI76_RS00265 at 54.568 kb on + strand, within BWI76_RS00265 at 54.568 kb on + strand, within BWI76_RS00265 at 54.576 kb on - strand at 54.618 kb on + strand, within BWI76_RS00265 at 54.687 kb on - strand, within BWI76_RS00265 at 54.803 kb on + strand, within BWI76_RS00265 at 54.838 kb on - strand, within BWI76_RS00265 at 54.854 kb on - strand, within BWI76_RS00265 at 54.924 kb on - strand, within BWI76_RS00265 at 55.033 kb on + strand, within BWI76_RS00265 at 55.033 kb on + strand, within BWI76_RS00265 at 55.041 kb on - strand, within BWI76_RS00265 at 55.041 kb on - strand, within BWI76_RS00265 at 55.046 kb on - strand, within BWI76_RS00265 at 55.156 kb on - strand, within BWI76_RS00265 at 55.172 kb on - strand, within BWI76_RS00265 at 55.192 kb on - strand, within BWI76_RS00265 at 55.260 kb on + strand, within BWI76_RS00265 at 55.336 kb on + strand, within BWI76_RS00265 at 55.336 kb on + strand, within BWI76_RS00265 at 55.336 kb on + strand at 55.336 kb on + strand, within BWI76_RS00265 at 55.344 kb on - strand, within BWI76_RS00265 at 55.358 kb on + strand, within BWI76_RS00265 at 55.360 kb on + strand, within BWI76_RS00265 at 55.368 kb on - strand, within BWI76_RS00265 at 55.368 kb on - strand, within BWI76_RS00265 at 55.580 kb on - strand, within BWI76_RS00265 at 55.697 kb on - strand, within BWI76_RS00265 at 55.728 kb on + strand, within BWI76_RS00265 at 55.745 kb on + strand, within BWI76_RS00265 at 55.810 kb on + strand, within BWI76_RS00265 at 55.932 kb on + strand, within BWI76_RS00265 at 55.932 kb on + strand, within BWI76_RS00265 at 55.940 kb on - strand at 56.062 kb on + strand at 56.370 kb on - strand, within BWI76_RS00270 at 56.429 kb on + strand, within BWI76_RS00270 at 56.435 kb on + strand, within BWI76_RS00270 at 56.540 kb on - strand, within BWI76_RS00270 at 56.568 kb on + strand, within BWI76_RS00270 at 56.576 kb on - strand, within BWI76_RS00270 at 56.576 kb on - strand, within BWI76_RS00270 at 56.576 kb on - strand, within BWI76_RS00270 at 56.578 kb on + strand, within BWI76_RS00270 at 56.738 kb on + strand at 56.747 kb on - strand at 56.943 kb on + strand, within BWI76_RS00275
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 53,362 + BWI76_RS00260 0.55 +0.8 53,417 - BWI76_RS00260 0.58 +2.8 53,417 - BWI76_RS00260 0.58 -0.1 53,441 + BWI76_RS00260 0.60 +0.2 53,457 - BWI76_RS00260 0.61 +0.5 53,671 - BWI76_RS00260 0.75 -0.8 53,687 + BWI76_RS00260 0.76 +0.2 53,855 - BWI76_RS00260 0.88 +0.3 53,951 - +0.4 53,951 - -2.1 53,951 - +1.0 53,993 + -1.1 54,028 + +0.9 54,089 - -0.4 54,093 + +0.0 54,142 + +0.5 54,142 + -0.2 54,142 + +2.2 54,142 + +0.3 54,150 - -0.3 54,163 + +1.4 54,184 + -0.8 54,342 + +0.5 54,344 + +0.9 54,351 - -0.9 54,351 - -0.5 54,392 + -0.5 54,506 - BWI76_RS00265 0.13 -0.0 54,568 + BWI76_RS00265 0.17 +0.9 54,568 + BWI76_RS00265 0.17 -0.1 54,576 - +6.2 54,618 + BWI76_RS00265 0.19 -0.7 54,687 - BWI76_RS00265 0.23 +1.2 54,803 + BWI76_RS00265 0.29 +0.4 54,838 - BWI76_RS00265 0.31 +3.2 54,854 - BWI76_RS00265 0.32 -2.0 54,924 - BWI76_RS00265 0.36 +0.9 55,033 + BWI76_RS00265 0.41 -0.0 55,033 + BWI76_RS00265 0.41 -3.5 55,041 - BWI76_RS00265 0.42 +1.2 55,041 - BWI76_RS00265 0.42 -1.3 55,046 - BWI76_RS00265 0.42 -3.4 55,156 - BWI76_RS00265 0.48 -0.9 55,172 - BWI76_RS00265 0.49 +0.6 55,192 - BWI76_RS00265 0.50 -0.8 55,260 + BWI76_RS00265 0.54 +0.4 55,336 + BWI76_RS00265 0.58 +0.2 55,336 + BWI76_RS00265 0.58 -0.5 55,336 + +0.5 55,336 + BWI76_RS00265 0.58 -0.2 55,344 - BWI76_RS00265 0.58 +1.6 55,358 + BWI76_RS00265 0.59 +1.4 55,360 + BWI76_RS00265 0.59 +0.7 55,368 - BWI76_RS00265 0.59 -0.5 55,368 - BWI76_RS00265 0.59 -0.2 55,580 - BWI76_RS00265 0.71 +0.2 55,697 - BWI76_RS00265 0.77 -1.3 55,728 + BWI76_RS00265 0.79 -1.3 55,745 + BWI76_RS00265 0.80 +0.6 55,810 + BWI76_RS00265 0.83 -0.5 55,932 + BWI76_RS00265 0.90 -2.0 55,932 + BWI76_RS00265 0.90 -0.6 55,940 - +0.6 56,062 + -0.1 56,370 - BWI76_RS00270 0.15 -0.8 56,429 + BWI76_RS00270 0.29 +0.4 56,435 + BWI76_RS00270 0.30 +0.3 56,540 - BWI76_RS00270 0.55 -1.1 56,568 + BWI76_RS00270 0.62 +0.7 56,576 - BWI76_RS00270 0.64 +1.6 56,576 - BWI76_RS00270 0.64 -1.1 56,576 - BWI76_RS00270 0.64 +0.5 56,578 + BWI76_RS00270 0.64 +0.1 56,738 + +0.6 56,747 - +0.5 56,943 + BWI76_RS00275 0.14 -1.2
Or see this region's nucleotide sequence