Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26280 and BWI76_RS26285 are separated by 23 nucleotides BWI76_RS26285 and BWI76_RS26290 overlap by 4 nucleotides BWI76_RS26290 and BWI76_RS26295 are separated by 1 nucleotides BWI76_RS26295 and BWI76_RS26300 overlap by 4 nucleotides
BWI76_RS26280: BWI76_RS26280 - hypothetical protein, at 5,440,122 to 5,440,442
_RS26280
BWI76_RS26285: BWI76_RS26285 - glycerophosphodiester phosphodiesterase, at 5,440,466 to 5,441,206
_RS26285
BWI76_RS26290: BWI76_RS26290 - glycerol-3-phosphate ABC transporter ATP-binding protein, at 5,441,203 to 5,442,273
_RS26290
BWI76_RS26295: BWI76_RS26295 - glycerol-3-phosphate transporter membrane protein, at 5,442,275 to 5,443,120
_RS26295
BWI76_RS26300: BWI76_RS26300 - glycerol-3-phosphate transporter permease, at 5,443,117 to 5,444,004
_RS26300
Position (kb) 5441
5442
5443 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 5440.218 kb on - strand, within BWI76_RS26280 at 5440.293 kb on + strand, within BWI76_RS26280 at 5440.293 kb on + strand, within BWI76_RS26280 at 5440.601 kb on - strand, within BWI76_RS26285 at 5440.635 kb on - strand, within BWI76_RS26285 at 5440.635 kb on - strand, within BWI76_RS26285 at 5440.698 kb on + strand, within BWI76_RS26285 at 5440.943 kb on - strand, within BWI76_RS26285 at 5441.105 kb on + strand, within BWI76_RS26285 at 5441.113 kb on - strand, within BWI76_RS26285 at 5441.472 kb on + strand, within BWI76_RS26290 at 5441.683 kb on - strand, within BWI76_RS26290 at 5441.707 kb on - strand, within BWI76_RS26290 at 5441.885 kb on + strand, within BWI76_RS26290 at 5441.912 kb on - strand at 5442.111 kb on + strand, within BWI76_RS26290 at 5442.208 kb on + strand at 5442.289 kb on + strand at 5442.291 kb on + strand at 5442.387 kb on + strand, within BWI76_RS26295 at 5442.400 kb on - strand, within BWI76_RS26295 at 5442.489 kb on - strand, within BWI76_RS26295 at 5442.517 kb on + strand, within BWI76_RS26295 at 5442.528 kb on + strand, within BWI76_RS26295 at 5442.546 kb on - strand, within BWI76_RS26295 at 5442.854 kb on - strand, within BWI76_RS26295 at 5443.010 kb on + strand, within BWI76_RS26295 at 5443.150 kb on + strand at 5443.167 kb on + strand at 5443.170 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 5,440,218 - BWI76_RS26280 0.30 -0.1 5,440,293 + BWI76_RS26280 0.53 -0.0 5,440,293 + BWI76_RS26280 0.53 -0.3 5,440,601 - BWI76_RS26285 0.18 +0.3 5,440,635 - BWI76_RS26285 0.23 -1.1 5,440,635 - BWI76_RS26285 0.23 +2.9 5,440,698 + BWI76_RS26285 0.31 +0.6 5,440,943 - BWI76_RS26285 0.64 -1.0 5,441,105 + BWI76_RS26285 0.86 -0.1 5,441,113 - BWI76_RS26285 0.87 +1.3 5,441,472 + BWI76_RS26290 0.25 -3.2 5,441,683 - BWI76_RS26290 0.45 +4.4 5,441,707 - BWI76_RS26290 0.47 -0.7 5,441,885 + BWI76_RS26290 0.64 -0.1 5,441,912 - +2.9 5,442,111 + BWI76_RS26290 0.85 -0.6 5,442,208 + -0.6 5,442,289 + -0.7 5,442,291 + -0.9 5,442,387 + BWI76_RS26295 0.13 +1.4 5,442,400 - BWI76_RS26295 0.15 +0.3 5,442,489 - BWI76_RS26295 0.25 -0.8 5,442,517 + BWI76_RS26295 0.29 -1.8 5,442,528 + BWI76_RS26295 0.30 +0.0 5,442,546 - BWI76_RS26295 0.32 -1.0 5,442,854 - BWI76_RS26295 0.68 +0.4 5,443,010 + BWI76_RS26295 0.87 +0.1 5,443,150 + +3.5 5,443,167 + +0.5 5,443,170 - -0.1
Or see this region's nucleotide sequence