Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25620 and BWI76_RS25625 are separated by 53 nucleotides BWI76_RS25625 and BWI76_RS25630 are separated by 21 nucleotides BWI76_RS25630 and BWI76_RS25635 overlap by 2 nucleotides BWI76_RS25635 and BWI76_RS25640 overlap by 25 nucleotides BWI76_RS25640 and BWI76_RS25645 overlap by 4 nucleotides
BWI76_RS25620: BWI76_RS25620 - 16S ribosomal RNA, at 5,323,839 to 5,325,392
_RS25620
BWI76_RS25625: BWI76_RS25625 - hypothetical protein, at 5,325,446 to 5,325,651
_RS25625
BWI76_RS25630: BWI76_RS25630 - hypothetical protein, at 5,325,673 to 5,325,861
_RS25630
BWI76_RS25635: BWI76_RS25635 - gamma carbonic anhydrase family protein, at 5,325,860 to 5,326,414
_RS25635
BWI76_RS25640: BWI76_RS25640 - hypothetical protein, at 5,326,390 to 5,326,647
_RS25640
BWI76_RS25645: BWI76_RS25645 - shikimate dehydrogenase, at 5,326,644 to 5,327,462
_RS25645
Position (kb) 5325
5326 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5325.798 kb on - strand, within BWI76_RS25630 at 5325.807 kb on - strand, within BWI76_RS25630 at 5326.136 kb on - strand, within BWI76_RS25635 at 5326.136 kb on - strand, within BWI76_RS25635 at 5326.264 kb on + strand at 5326.278 kb on + strand, within BWI76_RS25635 at 5326.287 kb on - strand, within BWI76_RS25635 at 5326.342 kb on + strand, within BWI76_RS25635 at 5326.350 kb on - strand, within BWI76_RS25635 at 5326.403 kb on + strand at 5326.403 kb on + strand at 5326.406 kb on - strand at 5326.426 kb on - strand, within BWI76_RS25640 at 5326.426 kb on - strand, within BWI76_RS25640 at 5326.491 kb on + strand, within BWI76_RS25640 at 5326.533 kb on + strand, within BWI76_RS25640 at 5326.548 kb on - strand, within BWI76_RS25640 at 5326.565 kb on - strand, within BWI76_RS25640 at 5326.670 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 5,325,798 - BWI76_RS25630 0.66 -1.4 5,325,807 - BWI76_RS25630 0.71 -1.9 5,326,136 - BWI76_RS25635 0.50 +1.4 5,326,136 - BWI76_RS25635 0.50 +0.3 5,326,264 + +2.9 5,326,278 + BWI76_RS25635 0.75 +1.6 5,326,287 - BWI76_RS25635 0.77 +1.7 5,326,342 + BWI76_RS25635 0.87 +1.3 5,326,350 - BWI76_RS25635 0.88 -0.3 5,326,403 + -0.4 5,326,403 + +1.8 5,326,406 - +0.5 5,326,426 - BWI76_RS25640 0.14 -1.0 5,326,426 - BWI76_RS25640 0.14 -1.7 5,326,491 + BWI76_RS25640 0.39 -0.7 5,326,533 + BWI76_RS25640 0.55 -0.3 5,326,548 - BWI76_RS25640 0.61 +0.8 5,326,565 - BWI76_RS25640 0.68 -1.8 5,326,670 - +0.9
Or see this region's nucleotide sequence