Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25520 and BWI76_RS25525 are separated by 121 nucleotides BWI76_RS25525 and BWI76_RS25530 are separated by 9 nucleotides BWI76_RS25530 and BWI76_RS25535 overlap by 28 nucleotides BWI76_RS25535 and BWI76_RS25540 are separated by 10 nucleotides BWI76_RS25540 and BWI76_RS25545 are separated by 110 nucleotides BWI76_RS25545 and BWI76_RS25550 overlap by 11 nucleotides
BWI76_RS25520: BWI76_RS25520 - sulfoxide reductase heme-binding subunit YedZ, at 5,306,066 to 5,306,665
_RS25520
BWI76_RS25525: BWI76_RS25525 - hypothetical protein, at 5,306,787 to 5,306,987
_RS25525
BWI76_RS25530: BWI76_RS25530 - acetyl-CoA carboxylase, at 5,306,997 to 5,307,086
_RS25530
BWI76_RS25535: BWI76_RS25535 - acetyl-CoA carboxylase biotin carboxyl carrier protein subunit, at 5,307,059 to 5,307,532
_RS25535
BWI76_RS25540: BWI76_RS25540 - acetyl-CoA carboxylase biotin carboxylase subunit, at 5,307,543 to 5,308,892
_RS25540
BWI76_RS25545: BWI76_RS25545 - hypothetical protein, at 5,309,003 to 5,309,245
_RS25545
BWI76_RS25550: BWI76_RS25550 - sodium:pantothenate symporter, at 5,309,235 to 5,310,686
_RS25550
Position (kb) 5307
5308
5309 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6
7 at 5306.575 kb on + strand, within BWI76_RS25520 at 5308.950 kb on + strand at 5309.026 kb on - strand at 5309.031 kb on - strand, within BWI76_RS25545 at 5309.136 kb on + strand, within BWI76_RS25545 at 5309.182 kb on + strand, within BWI76_RS25545 at 5309.192 kb on + strand, within BWI76_RS25545 at 5309.235 kb on - strand at 5309.294 kb on - strand at 5309.405 kb on - strand, within BWI76_RS25550 at 5309.405 kb on - strand, within BWI76_RS25550 at 5309.405 kb on - strand, within BWI76_RS25550 at 5309.744 kb on + strand, within BWI76_RS25550 at 5309.745 kb on + strand, within BWI76_RS25550 at 5309.747 kb on + strand, within BWI76_RS25550 at 5309.799 kb on + strand, within BWI76_RS25550 at 5309.807 kb on - strand, within BWI76_RS25550
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 5,306,575 + BWI76_RS25520 0.85 +1.1 5,308,950 + +0.4 5,309,026 - -2.5 5,309,031 - BWI76_RS25545 0.12 -0.3 5,309,136 + BWI76_RS25545 0.55 -2.9 5,309,182 + BWI76_RS25545 0.74 +0.9 5,309,192 + BWI76_RS25545 0.78 -0.8 5,309,235 - +7.2 5,309,294 - -1.0 5,309,405 - BWI76_RS25550 0.12 +1.1 5,309,405 - BWI76_RS25550 0.12 +1.2 5,309,405 - BWI76_RS25550 0.12 +0.1 5,309,744 + BWI76_RS25550 0.35 -0.4 5,309,745 + BWI76_RS25550 0.35 +2.1 5,309,747 + BWI76_RS25550 0.35 -0.1 5,309,799 + BWI76_RS25550 0.39 -1.0 5,309,807 - BWI76_RS25550 0.39 +0.4
Or see this region's nucleotide sequence