Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24820 and BWI76_RS24825 are separated by 65 nucleotides BWI76_RS24825 and BWI76_RS24830 are separated by 28 nucleotides BWI76_RS24830 and BWI76_RS24835 are separated by 28 nucleotides
BWI76_RS24820: BWI76_RS24820 - glycerate 2-kinase, at 5,171,423 to 5,172,568
_RS24820
BWI76_RS24825: BWI76_RS24825 - 2-hydroxy-3-oxopropionate reductase, at 5,172,634 to 5,173,524
_RS24825
BWI76_RS24830: BWI76_RS24830 - 5-keto-4-deoxy-D-glucarate aldolase, at 5,173,553 to 5,174,323
_RS24830
BWI76_RS24835: BWI76_RS24835 - MFS transporter, at 5,174,352 to 5,175,686
_RS24835
Position (kb) 5172
5173
5174 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5172.133 kb on - strand, within BWI76_RS24820 at 5172.248 kb on - strand, within BWI76_RS24820 at 5172.257 kb on + strand, within BWI76_RS24820 at 5172.271 kb on + strand, within BWI76_RS24820 at 5172.394 kb on - strand, within BWI76_RS24820 at 5172.511 kb on - strand at 5172.535 kb on + strand at 5172.604 kb on - strand at 5172.610 kb on - strand at 5172.627 kb on + strand at 5172.650 kb on + strand at 5172.892 kb on - strand, within BWI76_RS24825 at 5173.072 kb on - strand, within BWI76_RS24825 at 5173.533 kb on - strand at 5173.636 kb on - strand, within BWI76_RS24830 at 5173.639 kb on + strand, within BWI76_RS24830 at 5173.741 kb on + strand, within BWI76_RS24830 at 5173.777 kb on - strand, within BWI76_RS24830 at 5173.864 kb on - strand, within BWI76_RS24830 at 5173.923 kb on + strand, within BWI76_RS24830 at 5173.923 kb on + strand, within BWI76_RS24830 at 5174.191 kb on - strand, within BWI76_RS24830 at 5174.326 kb on + strand at 5174.334 kb on - strand at 5174.412 kb on + strand at 5174.446 kb on + strand at 5174.487 kb on - strand, within BWI76_RS24835
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 5,172,133 - BWI76_RS24820 0.62 -0.7 5,172,248 - BWI76_RS24820 0.72 +1.1 5,172,257 + BWI76_RS24820 0.73 +0.3 5,172,271 + BWI76_RS24820 0.74 -1.3 5,172,394 - BWI76_RS24820 0.85 +0.6 5,172,511 - -1.3 5,172,535 + -0.1 5,172,604 - +0.3 5,172,610 - +0.4 5,172,627 + -0.1 5,172,650 + -0.2 5,172,892 - BWI76_RS24825 0.29 +1.9 5,173,072 - BWI76_RS24825 0.49 -2.1 5,173,533 - -1.8 5,173,636 - BWI76_RS24830 0.11 -2.4 5,173,639 + BWI76_RS24830 0.11 +0.0 5,173,741 + BWI76_RS24830 0.24 +0.0 5,173,777 - BWI76_RS24830 0.29 -0.5 5,173,864 - BWI76_RS24830 0.40 +0.0 5,173,923 + BWI76_RS24830 0.48 +0.2 5,173,923 + BWI76_RS24830 0.48 +0.9 5,174,191 - BWI76_RS24830 0.83 -0.2 5,174,326 + -0.7 5,174,334 - -0.1 5,174,412 + +0.1 5,174,446 + +1.5 5,174,487 - BWI76_RS24835 0.10 +0.7
Or see this region's nucleotide sequence