Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23820 and BWI76_RS23825 are separated by 8 nucleotides BWI76_RS23825 and BWI76_RS23830 overlap by 20 nucleotides BWI76_RS23830 and BWI76_RS23835 are separated by 243 nucleotides
BWI76_RS23820: BWI76_RS23820 - flavodoxin FldB, at 4,972,790 to 4,973,311
_RS23820
BWI76_RS23825: BWI76_RS23825 - hypothetical protein, at 4,973,320 to 4,973,730
_RS23825
BWI76_RS23830: BWI76_RS23830 - hypothetical protein, at 4,973,711 to 4,973,977
_RS23830
BWI76_RS23835: BWI76_RS23835 - folate-binding protein, at 4,974,221 to 4,975,204
_RS23835
Position (kb) 4973
4974 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 4972.742 kb on + strand at 4972.866 kb on + strand, within BWI76_RS23820 at 4973.138 kb on + strand, within BWI76_RS23820 at 4973.139 kb on + strand, within BWI76_RS23820 at 4973.508 kb on + strand, within BWI76_RS23825 at 4973.516 kb on - strand, within BWI76_RS23825 at 4973.573 kb on + strand, within BWI76_RS23825 at 4973.581 kb on - strand, within BWI76_RS23825 at 4973.602 kb on + strand, within BWI76_RS23825 at 4973.606 kb on - strand, within BWI76_RS23825 at 4973.613 kb on + strand, within BWI76_RS23825 at 4973.623 kb on - strand, within BWI76_RS23825 at 4973.635 kb on - strand, within BWI76_RS23825 at 4973.773 kb on + strand, within BWI76_RS23830 at 4973.773 kb on + strand, within BWI76_RS23830 at 4973.868 kb on + strand, within BWI76_RS23830 at 4973.879 kb on + strand, within BWI76_RS23830 at 4973.931 kb on + strand, within BWI76_RS23830 at 4973.938 kb on + strand, within BWI76_RS23830 at 4974.039 kb on - strand at 4974.121 kb on + strand at 4974.129 kb on - strand at 4974.129 kb on - strand at 4974.206 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 4,972,742 + -0.9 4,972,866 + BWI76_RS23820 0.15 -1.0 4,973,138 + BWI76_RS23820 0.67 -0.8 4,973,139 + BWI76_RS23820 0.67 -0.9 4,973,508 + BWI76_RS23825 0.46 -0.4 4,973,516 - BWI76_RS23825 0.48 +0.0 4,973,573 + BWI76_RS23825 0.62 -0.7 4,973,581 - BWI76_RS23825 0.64 +0.4 4,973,602 + BWI76_RS23825 0.69 +0.6 4,973,606 - BWI76_RS23825 0.70 +0.4 4,973,613 + BWI76_RS23825 0.71 -0.1 4,973,623 - BWI76_RS23825 0.74 -0.3 4,973,635 - BWI76_RS23825 0.77 +1.0 4,973,773 + BWI76_RS23830 0.23 -1.4 4,973,773 + BWI76_RS23830 0.23 -4.3 4,973,868 + BWI76_RS23830 0.59 -0.6 4,973,879 + BWI76_RS23830 0.63 -2.6 4,973,931 + BWI76_RS23830 0.82 -2.2 4,973,938 + BWI76_RS23830 0.85 -1.7 4,974,039 - +0.8 4,974,121 + -0.9 4,974,129 - +2.1 4,974,129 - -2.7 4,974,206 + +2.0
Or see this region's nucleotide sequence