Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23180 and BWI76_RS23185 are separated by 2 nucleotides BWI76_RS23185 and BWI76_RS23190 overlap by 1 nucleotides BWI76_RS23190 and BWI76_RS23195 are separated by 4 nucleotides BWI76_RS23195 and BWI76_RS23200 overlap by 16 nucleotides
BWI76_RS23180: BWI76_RS23180 - NADH dehydrogenase, at 4,829,478 to 4,830,833
_RS23180
BWI76_RS23185: BWI76_RS23185 - propanediol utilization: polyhedral bodies pduT, at 4,830,836 to 4,831,390
_RS23185
BWI76_RS23190: BWI76_RS23190 - propanediol utilization protein PduU, at 4,831,390 to 4,831,740
_RS23190
BWI76_RS23195: BWI76_RS23195 - ethanolamine utilization protein EutP, at 4,831,745 to 4,832,197
_RS23195
BWI76_RS23200: BWI76_RS23200 - propionate kinase, at 4,832,182 to 4,833,396
_RS23200
Position (kb) 4831
4832
4833 Strain fitness (log2 ratio) -1
0
1
2
3 at 4830.821 kb on + strand at 4830.890 kb on - strand at 4831.000 kb on - strand, within BWI76_RS23185 at 4831.077 kb on - strand, within BWI76_RS23185 at 4831.094 kb on + strand, within BWI76_RS23185 at 4831.420 kb on + strand at 4831.420 kb on + strand at 4831.428 kb on - strand, within BWI76_RS23190 at 4831.469 kb on - strand, within BWI76_RS23190 at 4831.538 kb on + strand, within BWI76_RS23190 at 4831.538 kb on + strand, within BWI76_RS23190 at 4831.903 kb on + strand, within BWI76_RS23195 at 4831.911 kb on - strand, within BWI76_RS23195 at 4831.921 kb on - strand, within BWI76_RS23195 at 4832.055 kb on - strand, within BWI76_RS23195 at 4832.177 kb on + strand at 4832.253 kb on + strand at 4832.455 kb on + strand, within BWI76_RS23200 at 4832.711 kb on + strand, within BWI76_RS23200 at 4832.898 kb on + strand, within BWI76_RS23200 at 4832.933 kb on + strand, within BWI76_RS23200 at 4832.967 kb on + strand, within BWI76_RS23200
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 4,830,821 + +0.9 4,830,890 - -0.8 4,831,000 - BWI76_RS23185 0.30 -0.3 4,831,077 - BWI76_RS23185 0.43 +0.4 4,831,094 + BWI76_RS23185 0.46 -0.0 4,831,420 + -1.1 4,831,420 + -0.0 4,831,428 - BWI76_RS23190 0.11 +0.6 4,831,469 - BWI76_RS23190 0.23 -0.8 4,831,538 + BWI76_RS23190 0.42 +0.5 4,831,538 + BWI76_RS23190 0.42 -1.3 4,831,903 + BWI76_RS23195 0.35 -0.3 4,831,911 - BWI76_RS23195 0.37 +3.2 4,831,921 - BWI76_RS23195 0.39 -0.1 4,832,055 - BWI76_RS23195 0.68 +1.6 4,832,177 + +0.6 4,832,253 + -0.3 4,832,455 + BWI76_RS23200 0.22 +0.3 4,832,711 + BWI76_RS23200 0.44 -0.6 4,832,898 + BWI76_RS23200 0.59 -1.5 4,832,933 + BWI76_RS23200 0.62 -1.3 4,832,967 + BWI76_RS23200 0.65 -1.1
Or see this region's nucleotide sequence