Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20565

Experiment: Parafilmed volatile agar plate with no fungus

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20545 and BWI76_RS20550 are separated by 202 nucleotidesBWI76_RS20550 and BWI76_RS20555 are separated by 41 nucleotidesBWI76_RS20555 and BWI76_RS20560 are separated by 219 nucleotidesBWI76_RS20560 and BWI76_RS20565 are separated by 4 nucleotidesBWI76_RS20565 and BWI76_RS20570 are separated by 57 nucleotides BWI76_RS20545: BWI76_RS20545 - sensor domain-containing phosphodiesterase, at 4,303,715 to 4,305,895 _RS20545 BWI76_RS20550: BWI76_RS20550 - tRNA-Ala, at 4,306,098 to 4,306,173 _RS20550 BWI76_RS20555: BWI76_RS20555 - tRNA-Ala, at 4,306,215 to 4,306,290 _RS20555 BWI76_RS20560: BWI76_RS20560 - hypothetical protein, at 4,306,510 to 4,306,863 _RS20560 BWI76_RS20565: BWI76_RS20565 - hypothetical protein, at 4,306,868 to 4,307,263 _RS20565 BWI76_RS20570: BWI76_RS20570 - glutamate--tRNA ligase, at 4,307,321 to 4,308,739 _RS20570 Position (kb) 4306 4307 4308Strain fitness (log2 ratio) -1 0 1 2 3at 4305.878 kb on - strandat 4305.956 kb on + strandat 4306.006 kb on + strandat 4306.019 kb on - strandat 4306.021 kb on - strandat 4306.344 kb on + strandat 4306.509 kb on - strandat 4306.531 kb on - strandat 4306.563 kb on - strand, within BWI76_RS20560at 4306.637 kb on - strand, within BWI76_RS20560at 4306.639 kb on - strand, within BWI76_RS20560at 4307.110 kb on - strand, within BWI76_RS20565at 4307.155 kb on - strand, within BWI76_RS20565at 4307.185 kb on - strand, within BWI76_RS20565at 4307.247 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus
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4,305,878 - +0.3
4,305,956 + -0.5
4,306,006 + -0.8
4,306,019 - -0.1
4,306,021 - -0.8
4,306,344 + -0.8
4,306,509 - -0.5
4,306,531 - -1.1
4,306,563 - BWI76_RS20560 0.15 +0.2
4,306,637 - BWI76_RS20560 0.36 -0.1
4,306,639 - BWI76_RS20560 0.36 +0.1
4,307,110 - BWI76_RS20565 0.61 +3.3
4,307,155 - BWI76_RS20565 0.72 +0.8
4,307,185 - BWI76_RS20565 0.80 +1.1
4,307,247 - +0.5

Or see this region's nucleotide sequence