Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS19880 and BWI76_RS19885 are separated by 168 nucleotides BWI76_RS19885 and BWI76_RS19890 are separated by 76 nucleotides BWI76_RS19890 and BWI76_RS19895 overlap by 1 nucleotides
BWI76_RS19880: BWI76_RS19880 - magnesium transporter, at 4,156,788 to 4,158,224
_RS19880
BWI76_RS19885: BWI76_RS19885 - multidrug ABC transporter permease/ATP-binding protein, at 4,158,393 to 4,160,036
_RS19885
BWI76_RS19890: BWI76_RS19890 - alpha-ketoglutarate-dependent dioxygenase AlkB, at 4,160,113 to 4,160,763
_RS19890
BWI76_RS19895: BWI76_RS19895 - bifunctional transcriptional regulator/O6-methylguanine-DNA methyltransferase, at 4,160,763 to 4,161,827
_RS19895
Position (kb)
4158
4159
4160
4161 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 4157.562 kb on - strand, within BWI76_RS19880 at 4157.627 kb on - strand, within BWI76_RS19880 at 4157.835 kb on + strand, within BWI76_RS19880 at 4158.989 kb on - strand, within BWI76_RS19885 at 4159.072 kb on - strand, within BWI76_RS19885 at 4159.167 kb on + strand, within BWI76_RS19885 at 4159.190 kb on - strand, within BWI76_RS19885 at 4159.909 kb on + strand at 4159.943 kb on + strand at 4159.951 kb on - strand at 4159.951 kb on - strand at 4159.951 kb on - strand at 4159.963 kb on - strand at 4160.078 kb on - strand at 4160.201 kb on - strand, within BWI76_RS19890 at 4160.349 kb on + strand, within BWI76_RS19890 at 4160.604 kb on - strand, within BWI76_RS19890 at 4160.724 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 4,157,562 - BWI76_RS19880 0.54 +0.9 4,157,627 - BWI76_RS19880 0.58 +0.5 4,157,835 + BWI76_RS19880 0.73 -2.2 4,158,989 - BWI76_RS19885 0.36 +1.0 4,159,072 - BWI76_RS19885 0.41 +3.8 4,159,167 + BWI76_RS19885 0.47 +0.0 4,159,190 - BWI76_RS19885 0.48 +0.9 4,159,909 + +0.8 4,159,943 + +0.6 4,159,951 - -0.4 4,159,951 - -0.1 4,159,951 - +1.6 4,159,963 - -0.2 4,160,078 - -0.7 4,160,201 - BWI76_RS19890 0.14 +2.3 4,160,349 + BWI76_RS19890 0.36 +0.4 4,160,604 - BWI76_RS19890 0.75 +0.6 4,160,724 + -1.5
Or see this region's nucleotide sequence