Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS19825 and BWI76_RS19830 are separated by 80 nucleotides BWI76_RS19830 and BWI76_RS19835 are separated by 50 nucleotides BWI76_RS19835 and BWI76_RS19840 are separated by 179 nucleotides BWI76_RS19840 and BWI76_RS19845 are separated by 19 nucleotides
BWI76_RS19825: BWI76_RS19825 - DNA mismatch repair protein MutT, at 4,146,193 to 4,146,786
_RS19825
BWI76_RS19830: BWI76_RS19830 - DeoR family transcriptional regulator, at 4,146,867 to 4,147,625
_RS19830
BWI76_RS19835: BWI76_RS19835 - nucleoid-associated protein YejK, at 4,147,676 to 4,148,683
_RS19835
BWI76_RS19840: BWI76_RS19840 - hypothetical protein, at 4,148,863 to 4,149,090
_RS19840
BWI76_RS19845: BWI76_RS19845 - phosphatase, at 4,149,110 to 4,150,870
_RS19845
Position (kb)
4147
4148
4149 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4146.691 kb on + strand, within BWI76_RS19825 at 4147.569 kb on - strand at 4147.625 kb on + strand at 4147.628 kb on - strand at 4147.628 kb on - strand at 4147.717 kb on + strand at 4147.977 kb on + strand, within BWI76_RS19835 at 4147.995 kb on - strand, within BWI76_RS19835 at 4148.466 kb on + strand, within BWI76_RS19835 at 4148.466 kb on + strand, within BWI76_RS19835 at 4148.474 kb on - strand, within BWI76_RS19835 at 4148.829 kb on - strand at 4148.885 kb on + strand at 4148.948 kb on + strand, within BWI76_RS19840
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 4,146,691 + BWI76_RS19825 0.84 -0.0 4,147,569 - -0.5 4,147,625 + -0.4 4,147,628 - -0.4 4,147,628 - -0.6 4,147,717 + -2.2 4,147,977 + BWI76_RS19835 0.30 -2.1 4,147,995 - BWI76_RS19835 0.32 -0.6 4,148,466 + BWI76_RS19835 0.78 -1.8 4,148,466 + BWI76_RS19835 0.78 -2.8 4,148,474 - BWI76_RS19835 0.79 -1.5 4,148,829 - -1.7 4,148,885 + +0.0 4,148,948 + BWI76_RS19840 0.37 +0.3
Or see this region's nucleotide sequence