Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS18140

Experiment: Parafilmed volatile agar plate with no fungus

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS18130 and BWI76_RS18135 are separated by 78 nucleotidesBWI76_RS18135 and BWI76_RS18140 overlap by 1 nucleotidesBWI76_RS18140 and BWI76_RS18145 are separated by 198 nucleotides BWI76_RS18130: BWI76_RS18130 - zinc ABC transporter substrate-binding protein, at 3,768,651 to 3,769,595 _RS18130 BWI76_RS18135: BWI76_RS18135 - zinc ABC transporter ATP-binding protein ZnuC, at 3,769,674 to 3,770,426 _RS18135 BWI76_RS18140: BWI76_RS18140 - zinc ABC transporter permease, at 3,770,426 to 3,771,211 _RS18140 BWI76_RS18145: BWI76_RS18145 - Holliday junction branch migration DNA helicase RuvB, at 3,771,410 to 3,772,420 _RS18145 Position (kb) 3770 3771 3772Strain fitness (log2 ratio) -1 0 1 2 3at 3769.758 kb on - strand, within BWI76_RS18135at 3769.758 kb on - strand, within BWI76_RS18135at 3769.778 kb on + strand, within BWI76_RS18135at 3769.890 kb on - strand, within BWI76_RS18135at 3769.991 kb on - strand, within BWI76_RS18135at 3770.110 kb on + strand, within BWI76_RS18135at 3770.122 kb on + strand, within BWI76_RS18135at 3770.542 kb on - strand, within BWI76_RS18140at 3770.578 kb on + strand, within BWI76_RS18140at 3770.611 kb on - strand, within BWI76_RS18140at 3771.435 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus
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3,769,758 - BWI76_RS18135 0.11 +0.7
3,769,758 - BWI76_RS18135 0.11 +0.5
3,769,778 + BWI76_RS18135 0.14 -0.9
3,769,890 - BWI76_RS18135 0.29 -0.5
3,769,991 - BWI76_RS18135 0.42 +1.0
3,770,110 + BWI76_RS18135 0.58 -0.2
3,770,122 + BWI76_RS18135 0.59 +1.2
3,770,542 - BWI76_RS18140 0.15 -0.1
3,770,578 + BWI76_RS18140 0.19 +3.6
3,770,611 - BWI76_RS18140 0.24 +1.6
3,771,435 - -0.1

Or see this region's nucleotide sequence