Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS17525

Experiment: Parafilmed volatile agar plate with no fungus

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS17515 and BWI76_RS17520 are separated by 12 nucleotidesBWI76_RS17520 and BWI76_RS17525 are separated by 48 nucleotidesBWI76_RS17525 and BWI76_RS17530 overlap by 96 nucleotidesBWI76_RS17530 and BWI76_RS17535 are separated by 251 nucleotides BWI76_RS17515: BWI76_RS17515 - dihydropyrimidinase, at 3,632,423 to 3,633,829 _RS17515 BWI76_RS17520: BWI76_RS17520 - carbamate kinase, at 3,633,842 to 3,634,774 _RS17520 BWI76_RS17525: BWI76_RS17525 - XshC-Cox1-family protein, at 3,634,823 to 3,635,656 _RS17525 BWI76_RS17530: BWI76_RS17530 - hypothetical protein, at 3,635,561 to 3,635,938 _RS17530 BWI76_RS17535: BWI76_RS17535 - rRNA metabolism protein, at 3,636,190 to 3,639,294 _RS17535 Position (kb) 3634 3635 3636Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3633.904 kb on + strandat 3633.904 kb on + strandat 3634.180 kb on - strand, within BWI76_RS17520at 3634.396 kb on - strand, within BWI76_RS17520at 3634.433 kb on + strand, within BWI76_RS17520at 3634.472 kb on + strand, within BWI76_RS17520at 3634.782 kb on - strandat 3635.433 kb on - strand, within BWI76_RS17525at 3635.549 kb on + strand, within BWI76_RS17525at 3635.563 kb on + strandat 3635.571 kb on - strandat 3635.582 kb on + strandat 3635.590 kb on - strandat 3635.603 kb on + strandat 3635.613 kb on - strandat 3635.615 kb on + strandat 3635.623 kb on - strandat 3635.623 kb on - strandat 3635.623 kb on - strandat 3635.623 kb on - strandat 3635.631 kb on - strandat 3635.670 kb on - strand, within BWI76_RS17530at 3635.671 kb on - strand, within BWI76_RS17530at 3635.685 kb on + strand, within BWI76_RS17530at 3635.765 kb on + strand, within BWI76_RS17530at 3635.853 kb on + strand, within BWI76_RS17530at 3635.884 kb on + strand, within BWI76_RS17530at 3635.974 kb on + strandat 3635.982 kb on - strandat 3635.982 kb on - strandat 3636.000 kb on + strandat 3636.051 kb on + strandat 3636.059 kb on - strandat 3636.133 kb on + strandat 3636.147 kb on - strandat 3636.173 kb on + strandat 3636.175 kb on + strandat 3636.186 kb on + strandat 3636.249 kb on + strandat 3636.249 kb on + strandat 3636.249 kb on + strandat 3636.257 kb on - strandat 3636.331 kb on + strandat 3636.344 kb on - strandat 3636.349 kb on - strandat 3636.393 kb on - strandat 3636.432 kb on + strandat 3636.440 kb on - strandat 3636.474 kb on + strandat 3636.501 kb on + strand, within BWI76_RS17535at 3636.555 kb on + strand, within BWI76_RS17535at 3636.563 kb on - strand, within BWI76_RS17535at 3636.587 kb on + strand, within BWI76_RS17535at 3636.599 kb on - strand, within BWI76_RS17535at 3636.599 kb on - strand, within BWI76_RS17535

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus
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3,633,904 + -0.6
3,633,904 + +0.6
3,634,180 - BWI76_RS17520 0.36 +0.5
3,634,396 - BWI76_RS17520 0.59 -1.9
3,634,433 + BWI76_RS17520 0.63 -0.8
3,634,472 + BWI76_RS17520 0.68 +0.8
3,634,782 - -0.2
3,635,433 - BWI76_RS17525 0.73 +0.3
3,635,549 + BWI76_RS17525 0.87 +2.7
3,635,563 + -0.6
3,635,571 - +3.6
3,635,582 + +0.4
3,635,590 - -0.4
3,635,603 + +0.9
3,635,613 - +0.1
3,635,615 + -0.7
3,635,623 - -1.9
3,635,623 - +0.7
3,635,623 - -0.6
3,635,623 - -2.2
3,635,631 - -0.2
3,635,670 - BWI76_RS17530 0.29 +1.6
3,635,671 - BWI76_RS17530 0.29 +0.8
3,635,685 + BWI76_RS17530 0.33 -0.0
3,635,765 + BWI76_RS17530 0.54 +1.9
3,635,853 + BWI76_RS17530 0.77 +1.0
3,635,884 + BWI76_RS17530 0.85 +1.5
3,635,974 + -2.7
3,635,982 - -0.8
3,635,982 - -0.1
3,636,000 + -0.3
3,636,051 + -1.4
3,636,059 - +0.9
3,636,133 + -1.0
3,636,147 - -0.0
3,636,173 + -1.6
3,636,175 + +0.2
3,636,186 + +1.1
3,636,249 + -0.4
3,636,249 + +0.2
3,636,249 + +0.3
3,636,257 - -0.6
3,636,331 + -0.9
3,636,344 - +1.1
3,636,349 - -0.6
3,636,393 - +0.8
3,636,432 + +0.7
3,636,440 - -1.6
3,636,474 + +0.8
3,636,501 + BWI76_RS17535 0.10 -0.7
3,636,555 + BWI76_RS17535 0.12 +0.4
3,636,563 - BWI76_RS17535 0.12 -0.9
3,636,587 + BWI76_RS17535 0.13 -0.9
3,636,599 - BWI76_RS17535 0.13 -1.4
3,636,599 - BWI76_RS17535 0.13 +0.0

Or see this region's nucleotide sequence