Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16665 and BWI76_RS16670 are separated by 22 nucleotides BWI76_RS16670 and BWI76_RS16675 are separated by 62 nucleotides BWI76_RS16675 and BWI76_RS16680 are separated by 149 nucleotides
BWI76_RS16665: BWI76_RS16665 - ABC transporter permease, at 3,449,164 to 3,449,838
_RS16665
BWI76_RS16670: BWI76_RS16670 - NLPA lipoprotein, at 3,449,861 to 3,450,637
_RS16670
BWI76_RS16675: BWI76_RS16675 - ABC transporter, at 3,450,700 to 3,451,746
_RS16675
BWI76_RS16680: BWI76_RS16680 - ABC transporter substrate-binding protein, at 3,451,896 to 3,453,599
_RS16680
Position (kb) 3450
3451
3452 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6 at 3449.712 kb on - strand, within BWI76_RS16665 at 3449.880 kb on + strand at 3450.033 kb on + strand, within BWI76_RS16670 at 3450.041 kb on - strand, within BWI76_RS16670 at 3450.124 kb on - strand, within BWI76_RS16670 at 3450.255 kb on - strand, within BWI76_RS16670 at 3450.465 kb on + strand, within BWI76_RS16670 at 3450.581 kb on - strand at 3450.591 kb on + strand at 3450.761 kb on - strand at 3450.767 kb on - strand at 3451.022 kb on + strand, within BWI76_RS16675 at 3451.310 kb on + strand, within BWI76_RS16675 at 3451.884 kb on - strand at 3452.448 kb on - strand, within BWI76_RS16680 at 3452.687 kb on + strand, within BWI76_RS16680
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 3,449,712 - BWI76_RS16665 0.81 +0.3 3,449,880 + +0.1 3,450,033 + BWI76_RS16670 0.22 +1.3 3,450,041 - BWI76_RS16670 0.23 +0.2 3,450,124 - BWI76_RS16670 0.34 -1.0 3,450,255 - BWI76_RS16670 0.51 -0.5 3,450,465 + BWI76_RS16670 0.78 +0.4 3,450,581 - -0.5 3,450,591 + -0.0 3,450,761 - +6.2 3,450,767 - -0.2 3,451,022 + BWI76_RS16675 0.31 -0.9 3,451,310 + BWI76_RS16675 0.58 -1.8 3,451,884 - +0.4 3,452,448 - BWI76_RS16680 0.32 +0.1 3,452,687 + BWI76_RS16680 0.46 -0.3
Or see this region's nucleotide sequence