Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS16470

Experiment: Parafilmed volatile agar plate with no fungus

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS16460 and BWI76_RS16465 are separated by 48 nucleotidesBWI76_RS16465 and BWI76_RS16470 are separated by 142 nucleotidesBWI76_RS16470 and BWI76_RS16475 are separated by 53 nucleotides BWI76_RS16460: BWI76_RS16460 - oxidoreductase, at 3,414,116 to 3,415,012 _RS16460 BWI76_RS16465: BWI76_RS16465 - DUF1289 domain-containing protein, at 3,415,061 to 3,415,300 _RS16465 BWI76_RS16470: BWI76_RS16470 - transcriptional repressor NemR, at 3,415,443 to 3,416,042 _RS16470 BWI76_RS16475: BWI76_RS16475 - alkene reductase, at 3,416,096 to 3,417,193 _RS16475 Position (kb) 3415 3416 3417Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3414.814 kb on - strand, within BWI76_RS16460at 3414.983 kb on + strandat 3415.448 kb on + strandat 3415.499 kb on + strandat 3415.619 kb on + strand, within BWI76_RS16470at 3416.082 kb on + strandat 3416.187 kb on + strandat 3416.195 kb on - strandat 3416.213 kb on - strand, within BWI76_RS16475at 3416.290 kb on + strand, within BWI76_RS16475at 3416.325 kb on + strand, within BWI76_RS16475at 3416.325 kb on + strand, within BWI76_RS16475at 3416.333 kb on - strand, within BWI76_RS16475at 3416.717 kb on + strand, within BWI76_RS16475at 3416.725 kb on - strand, within BWI76_RS16475at 3417.036 kb on + strand, within BWI76_RS16475

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus
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3,414,814 - BWI76_RS16460 0.78 -0.1
3,414,983 + -2.2
3,415,448 + -1.3
3,415,499 + +0.0
3,415,619 + BWI76_RS16470 0.29 +1.0
3,416,082 + -2.6
3,416,187 + -2.8
3,416,195 - +0.5
3,416,213 - BWI76_RS16475 0.11 +0.5
3,416,290 + BWI76_RS16475 0.18 -0.3
3,416,325 + BWI76_RS16475 0.21 -0.6
3,416,325 + BWI76_RS16475 0.21 +0.3
3,416,333 - BWI76_RS16475 0.22 -0.1
3,416,717 + BWI76_RS16475 0.57 +1.1
3,416,725 - BWI76_RS16475 0.57 +1.9
3,417,036 + BWI76_RS16475 0.86 -2.2

Or see this region's nucleotide sequence