Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14320 and BWI76_RS14325 are separated by 7 nucleotides BWI76_RS14325 and BWI76_RS14330 are separated by 18 nucleotides BWI76_RS14330 and BWI76_RS14335 are separated by 804 nucleotides
BWI76_RS14320: BWI76_RS14320 - hypothetical protein, at 2,991,894 to 2,992,829
_RS14320
BWI76_RS14325: BWI76_RS14325 - maturase, at 2,992,837 to 2,992,957
_RS14325
BWI76_RS14330: BWI76_RS14330 - IS3 family transposase, at 2,992,976 to 2,993,797
_RS14330
BWI76_RS14335: BWI76_RS14335 - hypothetical protein, at 2,994,602 to 2,995,813
_RS14335
Position (kb)
2992
2993
2994 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2992.011 kb on + strand, within BWI76_RS14320 at 2992.114 kb on + strand, within BWI76_RS14320 at 2992.201 kb on + strand, within BWI76_RS14320 at 2992.293 kb on + strand at 2992.293 kb on + strand, within BWI76_RS14320 at 2992.395 kb on + strand, within BWI76_RS14320 at 2992.830 kb on + strand at 2992.894 kb on + strand, within BWI76_RS14325 at 2992.929 kb on - strand, within BWI76_RS14325 at 2992.932 kb on + strand, within BWI76_RS14325 at 2992.939 kb on + strand, within BWI76_RS14325 at 2992.945 kb on + strand, within BWI76_RS14325 at 2992.945 kb on + strand, within BWI76_RS14325 at 2992.945 kb on + strand, within BWI76_RS14325 at 2992.953 kb on + strand at 2992.989 kb on + strand at 2992.989 kb on + strand at 2993.024 kb on + strand at 2993.078 kb on - strand, within BWI76_RS14330 at 2993.080 kb on - strand at 2993.116 kb on + strand, within BWI76_RS14330 at 2993.159 kb on + strand, within BWI76_RS14330 at 2993.167 kb on - strand, within BWI76_RS14330 at 2993.191 kb on + strand, within BWI76_RS14330 at 2993.199 kb on - strand, within BWI76_RS14330 at 2993.224 kb on - strand, within BWI76_RS14330 at 2993.227 kb on + strand, within BWI76_RS14330 at 2993.227 kb on - strand, within BWI76_RS14330 at 2993.234 kb on - strand, within BWI76_RS14330 at 2993.235 kb on - strand, within BWI76_RS14330 at 2993.236 kb on + strand, within BWI76_RS14330 at 2993.236 kb on + strand, within BWI76_RS14330 at 2993.244 kb on - strand, within BWI76_RS14330 at 2993.317 kb on + strand, within BWI76_RS14330 at 2993.350 kb on + strand, within BWI76_RS14330 at 2993.418 kb on - strand at 2993.426 kb on - strand, within BWI76_RS14330 at 2993.467 kb on - strand, within BWI76_RS14330 at 2993.473 kb on + strand, within BWI76_RS14330 at 2993.749 kb on - strand at 2993.918 kb on - strand at 2993.969 kb on + strand at 2993.979 kb on + strand at 2993.987 kb on - strand at 2993.998 kb on + strand at 2994.025 kb on + strand at 2994.233 kb on + strand at 2994.284 kb on + strand at 2994.474 kb on + strand at 2994.496 kb on + strand at 2994.496 kb on + strand at 2994.504 kb on - strand at 2994.517 kb on + strand at 2994.607 kb on + strand at 2994.607 kb on + strand at 2994.609 kb on + strand at 2994.627 kb on - strand at 2994.654 kb on + strand at 2994.674 kb on + strand at 2994.682 kb on - strand at 2994.761 kb on - strand, within BWI76_RS14335
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 2,992,011 + BWI76_RS14320 0.12 -1.4 2,992,114 + BWI76_RS14320 0.24 -0.1 2,992,201 + BWI76_RS14320 0.33 -1.0 2,992,293 + -0.6 2,992,293 + BWI76_RS14320 0.43 -0.1 2,992,395 + BWI76_RS14320 0.54 -1.8 2,992,830 + -0.2 2,992,894 + BWI76_RS14325 0.47 +1.3 2,992,929 - BWI76_RS14325 0.76 +1.4 2,992,932 + BWI76_RS14325 0.79 +1.1 2,992,939 + BWI76_RS14325 0.84 -2.2 2,992,945 + BWI76_RS14325 0.89 -0.2 2,992,945 + BWI76_RS14325 0.89 -2.0 2,992,945 + BWI76_RS14325 0.89 -1.7 2,992,953 + +0.3 2,992,989 + +2.5 2,992,989 + +1.0 2,993,024 + -2.4 2,993,078 - BWI76_RS14330 0.12 +1.4 2,993,080 - +0.7 2,993,116 + BWI76_RS14330 0.17 +0.7 2,993,159 + BWI76_RS14330 0.22 +0.4 2,993,167 - BWI76_RS14330 0.23 +0.5 2,993,191 + BWI76_RS14330 0.26 -1.0 2,993,199 - BWI76_RS14330 0.27 +0.4 2,993,224 - BWI76_RS14330 0.30 +1.5 2,993,227 + BWI76_RS14330 0.31 +1.8 2,993,227 - BWI76_RS14330 0.31 +2.2 2,993,234 - BWI76_RS14330 0.31 +2.5 2,993,235 - BWI76_RS14330 0.32 +0.6 2,993,236 + BWI76_RS14330 0.32 -0.4 2,993,236 + BWI76_RS14330 0.32 +1.3 2,993,244 - BWI76_RS14330 0.33 -0.8 2,993,317 + BWI76_RS14330 0.41 -0.2 2,993,350 + BWI76_RS14330 0.45 +1.1 2,993,418 - -0.1 2,993,426 - BWI76_RS14330 0.55 -1.1 2,993,467 - BWI76_RS14330 0.60 +0.1 2,993,473 + BWI76_RS14330 0.60 -0.9 2,993,749 - -0.4 2,993,918 - -0.1 2,993,969 + -1.7 2,993,979 + -1.8 2,993,987 - -2.3 2,993,998 + +2.4 2,994,025 + +1.9 2,994,233 + -0.2 2,994,284 + +0.1 2,994,474 + -0.1 2,994,496 + -0.6 2,994,496 + +0.1 2,994,504 - -1.8 2,994,517 + -1.0 2,994,607 + -1.1 2,994,607 + +0.4 2,994,609 + +0.7 2,994,627 - -1.4 2,994,654 + -1.1 2,994,674 + +1.6 2,994,682 - -0.1 2,994,761 - BWI76_RS14335 0.13 -1.3
Or see this region's nucleotide sequence