Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS14280

Experiment: Parafilmed volatile agar plate with no fungus

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS14275 and BWI76_RS14280 are separated by 74 nucleotidesBWI76_RS14280 and BWI76_RS14285 are separated by 310 nucleotidesBWI76_RS14285 and BWI76_RS14290 are separated by 42 nucleotides BWI76_RS14275: BWI76_RS14275 - hypothetical protein, at 2,985,593 to 2,986,789 _RS14275 BWI76_RS14280: BWI76_RS14280 - hypothetical protein, at 2,986,864 to 2,987,121 _RS14280 BWI76_RS14285: BWI76_RS14285 - IS3 family transposase, at 2,987,432 to 2,987,722 _RS14285 BWI76_RS14290: BWI76_RS14290 - AAA family ATPase, at 2,987,765 to 2,988,368 _RS14290 Position (kb) 2986 2987 2988Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 2985.868 kb on + strand, within BWI76_RS14275at 2985.882 kb on - strand, within BWI76_RS14275at 2986.088 kb on + strand, within BWI76_RS14275at 2986.212 kb on + strand, within BWI76_RS14275at 2986.276 kb on + strand, within BWI76_RS14275at 2986.281 kb on + strand, within BWI76_RS14275at 2986.299 kb on - strand, within BWI76_RS14275at 2986.310 kb on + strand, within BWI76_RS14275at 2986.351 kb on + strand, within BWI76_RS14275at 2986.439 kb on - strand, within BWI76_RS14275at 2986.457 kb on - strand, within BWI76_RS14275at 2986.542 kb on - strand, within BWI76_RS14275at 2986.613 kb on - strand, within BWI76_RS14275at 2986.614 kb on + strand, within BWI76_RS14275at 2986.839 kb on - strandat 2986.875 kb on + strandat 2986.942 kb on + strand, within BWI76_RS14280at 2986.993 kb on + strand, within BWI76_RS14280at 2987.043 kb on - strand, within BWI76_RS14280at 2987.173 kb on + strandat 2987.185 kb on - strandat 2987.194 kb on + strandat 2987.194 kb on + strandat 2987.194 kb on + strandat 2987.194 kb on + strandat 2987.194 kb on + strandat 2987.194 kb on + strandat 2987.194 kb on + strandat 2987.194 kb on + strandat 2987.194 kb on + strandat 2987.194 kb on + strandat 2987.194 kb on + strandat 2987.194 kb on + strandat 2987.194 kb on + strandat 2987.194 kb on + strandat 2987.194 kb on + strandat 2987.202 kb on - strandat 2987.202 kb on - strandat 2987.202 kb on - strandat 2987.202 kb on - strandat 2987.202 kb on - strandat 2987.202 kb on - strandat 2987.202 kb on - strandat 2987.202 kb on - strandat 2987.202 kb on - strandat 2987.202 kb on - strandat 2987.202 kb on - strandat 2987.250 kb on - strandat 2987.275 kb on + strandat 2987.276 kb on + strandat 2987.293 kb on - strandat 2987.401 kb on + strandat 2987.446 kb on + strandat 2987.481 kb on - strand, within BWI76_RS14285at 2987.491 kb on - strand, within BWI76_RS14285at 2987.514 kb on - strand, within BWI76_RS14285at 2987.520 kb on + strand, within BWI76_RS14285at 2987.524 kb on + strand, within BWI76_RS14285at 2987.534 kb on + strand, within BWI76_RS14285at 2987.534 kb on + strand, within BWI76_RS14285at 2987.878 kb on + strand, within BWI76_RS14290at 2987.886 kb on - strand, within BWI76_RS14290at 2987.898 kb on + strand, within BWI76_RS14290at 2987.921 kb on + strand, within BWI76_RS14290at 2987.955 kb on + strand, within BWI76_RS14290at 2988.029 kb on + strand, within BWI76_RS14290at 2988.079 kb on + strand, within BWI76_RS14290

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus
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2,985,868 + BWI76_RS14275 0.23 +1.1
2,985,882 - BWI76_RS14275 0.24 +1.3
2,986,088 + BWI76_RS14275 0.41 +1.4
2,986,212 + BWI76_RS14275 0.52 +0.2
2,986,276 + BWI76_RS14275 0.57 -0.5
2,986,281 + BWI76_RS14275 0.57 -0.3
2,986,299 - BWI76_RS14275 0.59 -0.5
2,986,310 + BWI76_RS14275 0.60 -0.5
2,986,351 + BWI76_RS14275 0.63 +1.0
2,986,439 - BWI76_RS14275 0.71 -0.2
2,986,457 - BWI76_RS14275 0.72 -0.4
2,986,542 - BWI76_RS14275 0.79 +1.0
2,986,613 - BWI76_RS14275 0.85 -0.5
2,986,614 + BWI76_RS14275 0.85 -0.8
2,986,839 - +0.5
2,986,875 + +2.4
2,986,942 + BWI76_RS14280 0.30 +0.9
2,986,993 + BWI76_RS14280 0.50 -0.1
2,987,043 - BWI76_RS14280 0.69 +1.8
2,987,173 + -1.0
2,987,185 - -0.6
2,987,194 + -0.6
2,987,194 + +0.2
2,987,194 + +1.2
2,987,194 + -0.2
2,987,194 + -0.1
2,987,194 + +3.3
2,987,194 + -2.8
2,987,194 + -0.4
2,987,194 + -1.1
2,987,194 + -2.0
2,987,194 + +1.0
2,987,194 + +0.5
2,987,194 + +0.8
2,987,194 + -0.2
2,987,194 + -2.5
2,987,202 - -2.1
2,987,202 - -0.7
2,987,202 - -0.1
2,987,202 - -1.4
2,987,202 - -1.1
2,987,202 - -0.1
2,987,202 - +0.8
2,987,202 - -0.2
2,987,202 - -0.7
2,987,202 - -0.7
2,987,202 - -1.5
2,987,250 - -0.1
2,987,275 + +0.3
2,987,276 + -0.6
2,987,293 - -0.2
2,987,401 + -1.5
2,987,446 + -1.1
2,987,481 - BWI76_RS14285 0.17 -2.0
2,987,491 - BWI76_RS14285 0.20 +1.3
2,987,514 - BWI76_RS14285 0.28 -0.3
2,987,520 + BWI76_RS14285 0.30 -0.4
2,987,524 + BWI76_RS14285 0.32 -2.7
2,987,534 + BWI76_RS14285 0.35 -1.1
2,987,534 + BWI76_RS14285 0.35 +3.2
2,987,878 + BWI76_RS14290 0.19 -1.3
2,987,886 - BWI76_RS14290 0.20 +0.7
2,987,898 + BWI76_RS14290 0.22 -0.9
2,987,921 + BWI76_RS14290 0.26 -0.2
2,987,955 + BWI76_RS14290 0.31 -0.7
2,988,029 + BWI76_RS14290 0.44 +1.6
2,988,079 + BWI76_RS14290 0.52 +0.5

Or see this region's nucleotide sequence