Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13960 and BWI76_RS13965 are separated by 24 nucleotides BWI76_RS13965 and BWI76_RS13970 overlap by 4 nucleotides BWI76_RS13970 and BWI76_RS13975 are separated by 4 nucleotides BWI76_RS13975 and BWI76_RS13980 are separated by 10 nucleotides
BWI76_RS13960: BWI76_RS13960 - urea ABC transporter periplasmic protein, at 2,914,072 to 2,915,130
_RS13960
BWI76_RS13965: BWI76_RS13965 - binding-protein-dependent transport system inner membrane protein, at 2,915,155 to 2,915,970
_RS13965
BWI76_RS13970: BWI76_RS13970 - ABC transporter, at 2,915,967 to 2,916,743
_RS13970
BWI76_RS13975: BWI76_RS13975 - urea carboxylase, at 2,916,748 to 2,917,476
_RS13975
BWI76_RS13980: BWI76_RS13980 - hypothetical protein, at 2,917,487 to 2,918,122
_RS13980
Position (kb)
2915
2916
2917 Strain fitness (log2 ratio)
-1
0
1
2 at 2915.563 kb on - strand, within BWI76_RS13965 at 2915.752 kb on - strand, within BWI76_RS13965 at 2916.018 kb on + strand at 2916.026 kb on - strand at 2916.576 kb on + strand, within BWI76_RS13970 at 2916.777 kb on - strand at 2916.778 kb on - strand at 2917.061 kb on - strand, within BWI76_RS13975 at 2917.396 kb on + strand, within BWI76_RS13975
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 2,915,563 - BWI76_RS13965 0.50 +0.6 2,915,752 - BWI76_RS13965 0.73 -0.8 2,916,018 + +2.2 2,916,026 - -0.7 2,916,576 + BWI76_RS13970 0.78 -1.3 2,916,777 - -0.4 2,916,778 - +0.4 2,917,061 - BWI76_RS13975 0.43 -0.2 2,917,396 + BWI76_RS13975 0.89 -0.3
Or see this region's nucleotide sequence