Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS13120

Experiment: Parafilmed volatile agar plate with no fungus

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS13110 and BWI76_RS13115 overlap by 4 nucleotidesBWI76_RS13115 and BWI76_RS13120 are separated by 2 nucleotidesBWI76_RS13120 and BWI76_RS13125 are separated by 3 nucleotides BWI76_RS13110: BWI76_RS13110 - phenylacetic acid degradation protein, at 2,734,299 to 2,735,369 _RS13110 BWI76_RS13115: BWI76_RS13115 - 2,3-dehydroadipyl-CoA hydratase, at 2,735,366 to 2,736,133 _RS13115 BWI76_RS13120: BWI76_RS13120 - 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase, at 2,736,136 to 2,736,924 _RS13120 BWI76_RS13125: BWI76_RS13125 - 3-hydroxyacyl-CoA dehydrogenase, at 2,736,928 to 2,738,349 _RS13125 Position (kb) 2736 2737Strain fitness (log2 ratio) -1 0 1at 2735.337 kb on + strandat 2735.375 kb on - strandat 2736.150 kb on - strandat 2736.166 kb on - strandat 2736.758 kb on + strand, within BWI76_RS13120at 2736.963 kb on - strandat 2737.662 kb on + strand, within BWI76_RS13125at 2737.786 kb on + strand, within BWI76_RS13125at 2737.794 kb on - strand, within BWI76_RS13125at 2737.877 kb on + strand, within BWI76_RS13125

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus
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2,735,337 + +0.6
2,735,375 - -1.1
2,736,150 - +1.5
2,736,166 - -0.1
2,736,758 + BWI76_RS13120 0.79 -1.3
2,736,963 - -0.1
2,737,662 + BWI76_RS13125 0.52 +0.0
2,737,786 + BWI76_RS13125 0.60 -1.5
2,737,794 - BWI76_RS13125 0.61 +1.6
2,737,877 + BWI76_RS13125 0.67 -0.2

Or see this region's nucleotide sequence