Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12555 and BWI76_RS12560 overlap by 4 nucleotides BWI76_RS12560 and BWI76_RS12565 overlap by 4 nucleotides BWI76_RS12565 and BWI76_RS12570 overlap by 4 nucleotides BWI76_RS12570 and BWI76_RS12575 are separated by 1 nucleotides
BWI76_RS12550: BWI76_RS12550 - hypothetical protein, at 2,621,981 to 2,622,514
_RS12550
BWI76_RS12555: BWI76_RS12555 - hypothetical protein, at 2,622,511 to 2,623,035
_RS12555
BWI76_RS12560: BWI76_RS12560 - hypothetical protein, at 2,623,032 to 2,623,517
_RS12560
BWI76_RS12565: BWI76_RS12565 - SAM-dependent methyltransferase, at 2,623,514 to 2,624,734
_RS12565
BWI76_RS12570: BWI76_RS12570 - DUF1365 domain-containing protein, at 2,624,731 to 2,625,453
_RS12570
BWI76_RS12575: BWI76_RS12575 - FAD-dependent oxidoreductase, at 2,625,455 to 2,626,714
_RS12575
Position (kb)
2623
2624
2625 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2622.699 kb on - strand, within BWI76_RS12555 at 2622.699 kb on - strand, within BWI76_RS12555 at 2622.806 kb on + strand, within BWI76_RS12555 at 2622.814 kb on - strand, within BWI76_RS12555 at 2622.838 kb on - strand, within BWI76_RS12555 at 2623.224 kb on + strand at 2623.615 kb on + strand at 2623.691 kb on + strand, within BWI76_RS12565 at 2623.995 kb on + strand, within BWI76_RS12565 at 2624.000 kb on + strand, within BWI76_RS12565 at 2624.249 kb on - strand, within BWI76_RS12565 at 2624.267 kb on - strand, within BWI76_RS12565 at 2624.495 kb on - strand, within BWI76_RS12565 at 2624.791 kb on - strand at 2624.865 kb on + strand, within BWI76_RS12570 at 2625.129 kb on + strand, within BWI76_RS12570 at 2625.533 kb on - strand at 2625.567 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 2,622,699 - BWI76_RS12555 0.36 -0.5 2,622,699 - BWI76_RS12555 0.36 +0.2 2,622,806 + BWI76_RS12555 0.56 -1.5 2,622,814 - BWI76_RS12555 0.58 -0.0 2,622,838 - BWI76_RS12555 0.62 +0.9 2,623,224 + +1.8 2,623,615 + -0.0 2,623,691 + BWI76_RS12565 0.14 +2.8 2,623,995 + BWI76_RS12565 0.39 -0.0 2,624,000 + BWI76_RS12565 0.40 +2.6 2,624,249 - BWI76_RS12565 0.60 +0.0 2,624,267 - BWI76_RS12565 0.62 +0.9 2,624,495 - BWI76_RS12565 0.80 -1.6 2,624,791 - -0.1 2,624,865 + BWI76_RS12570 0.19 +0.3 2,625,129 + BWI76_RS12570 0.55 +0.6 2,625,533 - -0.1 2,625,567 - +0.9
Or see this region's nucleotide sequence