Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12375 and BWI76_RS12380 are separated by 488 nucleotides BWI76_RS12380 and BWI76_RS12385 are separated by 1 nucleotides BWI76_RS12385 and BWI76_RS12390 are separated by 135 nucleotides BWI76_RS12390 and BWI76_RS12395 are separated by 349 nucleotides
BWI76_RS12375: BWI76_RS12375 - DNA-invertase hin, at 2,585,407 to 2,585,958
_RS12375
BWI76_RS12380: BWI76_RS12380 - DNA polymerase V subunit UmuD, at 2,586,447 to 2,586,866
_RS12380
BWI76_RS12385: BWI76_RS12385 - DNA polymerase V subunit UmuC, at 2,586,868 to 2,588,133
_RS12385
BWI76_RS12390: BWI76_RS12390 - antibiotic biosynthesis monooxygenase, at 2,588,269 to 2,588,568
_RS12390
BWI76_RS12395: BWI76_RS12395 - zinc-binding alcohol dehydrogenase, at 2,588,918 to 2,589,901
_RS12395
Position (kb)
2586
2587
2588
2589 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2586.058 kb on - strand at 2586.071 kb on + strand at 2586.188 kb on - strand at 2586.460 kb on - strand at 2586.478 kb on + strand at 2586.505 kb on + strand, within BWI76_RS12380 at 2586.547 kb on - strand, within BWI76_RS12380 at 2586.570 kb on - strand, within BWI76_RS12380 at 2586.582 kb on + strand, within BWI76_RS12380 at 2586.687 kb on + strand, within BWI76_RS12380 at 2586.758 kb on + strand, within BWI76_RS12380 at 2586.764 kb on + strand, within BWI76_RS12380 at 2586.825 kb on - strand at 2586.827 kb on + strand at 2587.122 kb on - strand, within BWI76_RS12385 at 2587.243 kb on + strand, within BWI76_RS12385 at 2587.287 kb on + strand, within BWI76_RS12385 at 2587.321 kb on + strand, within BWI76_RS12385 at 2587.367 kb on + strand, within BWI76_RS12385 at 2587.471 kb on + strand, within BWI76_RS12385 at 2587.548 kb on - strand, within BWI76_RS12385 at 2587.560 kb on + strand, within BWI76_RS12385 at 2587.568 kb on - strand at 2587.810 kb on + strand, within BWI76_RS12385 at 2587.963 kb on + strand, within BWI76_RS12385 at 2588.096 kb on + strand at 2588.104 kb on - strand at 2588.116 kb on - strand at 2588.230 kb on - strand at 2588.365 kb on + strand at 2588.435 kb on + strand, within BWI76_RS12390 at 2588.494 kb on + strand, within BWI76_RS12390 at 2588.624 kb on - strand at 2588.624 kb on - strand at 2588.649 kb on - strand at 2588.675 kb on - strand at 2588.710 kb on + strand at 2588.951 kb on + strand at 2588.994 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 2,586,058 - -0.1 2,586,071 + +0.9 2,586,188 - -0.2 2,586,460 - -0.0 2,586,478 + -0.9 2,586,505 + BWI76_RS12380 0.14 +1.7 2,586,547 - BWI76_RS12380 0.24 +0.7 2,586,570 - BWI76_RS12380 0.29 -0.2 2,586,582 + BWI76_RS12380 0.32 -0.3 2,586,687 + BWI76_RS12380 0.57 -0.7 2,586,758 + BWI76_RS12380 0.74 +0.1 2,586,764 + BWI76_RS12380 0.75 -0.2 2,586,825 - -0.7 2,586,827 + -0.1 2,587,122 - BWI76_RS12385 0.20 +4.0 2,587,243 + BWI76_RS12385 0.30 -0.9 2,587,287 + BWI76_RS12385 0.33 +1.4 2,587,321 + BWI76_RS12385 0.36 -1.1 2,587,367 + BWI76_RS12385 0.39 +1.8 2,587,471 + BWI76_RS12385 0.48 +0.6 2,587,548 - BWI76_RS12385 0.54 +1.2 2,587,560 + BWI76_RS12385 0.55 +0.3 2,587,568 - +0.8 2,587,810 + BWI76_RS12385 0.74 +0.4 2,587,963 + BWI76_RS12385 0.86 -1.2 2,588,096 + +0.2 2,588,104 - +2.7 2,588,116 - -1.7 2,588,230 - +1.7 2,588,365 + -0.5 2,588,435 + BWI76_RS12390 0.55 +0.6 2,588,494 + BWI76_RS12390 0.75 -0.7 2,588,624 - +0.0 2,588,624 - +0.5 2,588,649 - +0.1 2,588,675 - -0.3 2,588,710 + +1.6 2,588,951 + -1.8 2,588,994 - -0.1
Or see this region's nucleotide sequence