Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12205 and BWI76_RS12210 are separated by 810 nucleotides BWI76_RS12210 and BWI76_RS12215 are separated by 61 nucleotides BWI76_RS12215 and BWI76_RS12220 are separated by 389 nucleotides
BWI76_RS12205: BWI76_RS12205 - hypothetical protein, at 2,553,849 to 2,554,133
_RS12205
BWI76_RS12210: BWI76_RS12210 - hypothetical protein, at 2,554,944 to 2,555,189
_RS12210
BWI76_RS12215: BWI76_RS12215 - HNH endonuclease, at 2,555,251 to 2,555,376
_RS12215
BWI76_RS12220: BWI76_RS12220 - hypothetical protein, at 2,555,766 to 2,556,005
_RS12220
Position (kb)
2554
2555
2556 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6
7
8
9
10 at 2553.968 kb on + strand, within BWI76_RS12205 at 2554.104 kb on + strand, within BWI76_RS12205 at 2554.127 kb on + strand at 2554.152 kb on + strand at 2554.160 kb on - strand at 2554.160 kb on - strand at 2554.160 kb on - strand at 2554.237 kb on + strand at 2554.245 kb on - strand at 2554.255 kb on - strand at 2554.279 kb on + strand at 2554.287 kb on - strand at 2554.287 kb on - strand at 2554.293 kb on - strand at 2554.294 kb on + strand at 2554.303 kb on + strand at 2554.308 kb on + strand at 2554.391 kb on + strand at 2554.399 kb on - strand at 2554.406 kb on - strand at 2554.406 kb on - strand at 2554.469 kb on - strand at 2554.505 kb on - strand at 2554.511 kb on - strand at 2554.513 kb on - strand at 2554.513 kb on - strand at 2554.516 kb on + strand at 2554.516 kb on + strand at 2554.516 kb on + strand at 2554.516 kb on + strand at 2554.516 kb on + strand at 2554.533 kb on - strand at 2554.543 kb on - strand at 2554.565 kb on + strand at 2554.571 kb on + strand at 2554.580 kb on + strand at 2554.580 kb on + strand at 2554.588 kb on - strand at 2554.689 kb on + strand at 2554.690 kb on + strand at 2554.694 kb on + strand at 2554.694 kb on + strand at 2554.699 kb on + strand at 2554.720 kb on - strand at 2554.730 kb on - strand at 2554.744 kb on + strand at 2554.798 kb on + strand at 2554.860 kb on - strand at 2554.865 kb on - strand at 2554.894 kb on + strand at 2554.929 kb on + strand at 2554.936 kb on + strand at 2554.936 kb on + strand at 2554.978 kb on - strand, within BWI76_RS12210 at 2554.985 kb on + strand, within BWI76_RS12210 at 2554.992 kb on + strand, within BWI76_RS12210 at 2555.000 kb on - strand, within BWI76_RS12210 at 2555.000 kb on - strand, within BWI76_RS12210 at 2555.000 kb on - strand, within BWI76_RS12210 at 2555.037 kb on + strand, within BWI76_RS12210 at 2555.045 kb on - strand at 2555.076 kb on + strand, within BWI76_RS12210 at 2555.087 kb on + strand, within BWI76_RS12210 at 2555.139 kb on - strand, within BWI76_RS12210 at 2555.159 kb on + strand, within BWI76_RS12210 at 2555.167 kb on - strand at 2555.170 kb on + strand at 2555.170 kb on + strand at 2555.178 kb on - strand at 2555.178 kb on - strand at 2555.178 kb on - strand at 2555.192 kb on - strand at 2555.242 kb on + strand at 2555.270 kb on - strand, within BWI76_RS12215 at 2555.312 kb on + strand, within BWI76_RS12215 at 2555.312 kb on + strand, within BWI76_RS12215 at 2555.320 kb on - strand, within BWI76_RS12215 at 2555.329 kb on - strand, within BWI76_RS12215 at 2555.342 kb on - strand, within BWI76_RS12215 at 2555.353 kb on - strand, within BWI76_RS12215 at 2555.354 kb on + strand, within BWI76_RS12215 at 2555.362 kb on - strand, within BWI76_RS12215 at 2555.372 kb on - strand at 2555.381 kb on + strand at 2555.387 kb on - strand at 2555.416 kb on - strand at 2555.421 kb on - strand at 2555.446 kb on - strand at 2555.470 kb on - strand at 2555.511 kb on - strand at 2555.548 kb on + strand at 2555.548 kb on + strand at 2555.554 kb on - strand at 2555.556 kb on - strand at 2555.561 kb on + strand at 2555.591 kb on + strand at 2555.612 kb on - strand at 2555.628 kb on + strand at 2555.641 kb on + strand at 2555.652 kb on + strand at 2555.694 kb on + strand at 2555.694 kb on + strand at 2555.797 kb on + strand, within BWI76_RS12220 at 2555.808 kb on - strand, within BWI76_RS12220 at 2555.814 kb on + strand, within BWI76_RS12220 at 2555.814 kb on + strand, within BWI76_RS12220 at 2555.817 kb on - strand, within BWI76_RS12220 at 2555.817 kb on - strand, within BWI76_RS12220 at 2555.819 kb on + strand, within BWI76_RS12220 at 2555.822 kb on - strand, within BWI76_RS12220 at 2555.840 kb on + strand, within BWI76_RS12220 at 2555.848 kb on - strand, within BWI76_RS12220 at 2555.848 kb on - strand, within BWI76_RS12220 at 2555.884 kb on + strand, within BWI76_RS12220 at 2555.884 kb on + strand, within BWI76_RS12220 at 2555.892 kb on - strand, within BWI76_RS12220 at 2555.892 kb on - strand, within BWI76_RS12220 at 2555.892 kb on - strand, within BWI76_RS12220 at 2555.908 kb on - strand, within BWI76_RS12220 at 2555.951 kb on + strand, within BWI76_RS12220 at 2555.951 kb on + strand, within BWI76_RS12220 at 2555.951 kb on + strand, within BWI76_RS12220 at 2555.951 kb on + strand, within BWI76_RS12220 at 2555.961 kb on - strand, within BWI76_RS12220 at 2556.091 kb on + strand at 2556.091 kb on + strand at 2556.091 kb on + strand at 2556.091 kb on + strand at 2556.091 kb on + strand at 2556.091 kb on + strand at 2556.099 kb on + strand at 2556.099 kb on - strand at 2556.100 kb on + strand at 2556.101 kb on + strand at 2556.101 kb on + strand at 2556.105 kb on + strand at 2556.114 kb on + strand at 2556.114 kb on + strand at 2556.114 kb on + strand at 2556.122 kb on - strand at 2556.137 kb on + strand at 2556.140 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 2,553,968 + BWI76_RS12205 0.42 +2.0 2,554,104 + BWI76_RS12205 0.89 +0.8 2,554,127 + +1.1 2,554,152 + +1.0 2,554,160 - +0.0 2,554,160 - -0.3 2,554,160 - +1.2 2,554,237 + +1.8 2,554,245 - -0.1 2,554,255 - -0.3 2,554,279 + +0.2 2,554,287 - -1.0 2,554,287 - -1.1 2,554,293 - +0.5 2,554,294 + -0.7 2,554,303 + +0.1 2,554,308 + -0.1 2,554,391 + -2.0 2,554,399 - +1.5 2,554,406 - -1.0 2,554,406 - -0.4 2,554,469 - -1.8 2,554,505 - +0.5 2,554,511 - +0.2 2,554,513 - -0.6 2,554,513 - -0.8 2,554,516 + +0.5 2,554,516 + -1.2 2,554,516 + -0.3 2,554,516 + -0.8 2,554,516 + +0.7 2,554,533 - -1.6 2,554,543 - +0.8 2,554,565 + +3.5 2,554,571 + +0.8 2,554,580 + +0.2 2,554,580 + +0.3 2,554,588 - +0.5 2,554,689 + -0.5 2,554,690 + +1.4 2,554,694 + +0.9 2,554,694 + -0.0 2,554,699 + -1.2 2,554,720 - -0.9 2,554,730 - +1.5 2,554,744 + +0.6 2,554,798 + -0.3 2,554,860 - -1.2 2,554,865 - +1.2 2,554,894 + +0.1 2,554,929 + +0.1 2,554,936 + +0.6 2,554,936 + -0.0 2,554,978 - BWI76_RS12210 0.14 -0.5 2,554,985 + BWI76_RS12210 0.17 -0.1 2,554,992 + BWI76_RS12210 0.20 +0.5 2,555,000 - BWI76_RS12210 0.23 -0.7 2,555,000 - BWI76_RS12210 0.23 +10.7 2,555,000 - BWI76_RS12210 0.23 -0.1 2,555,037 + BWI76_RS12210 0.38 +0.5 2,555,045 - +2.8 2,555,076 + BWI76_RS12210 0.54 +0.2 2,555,087 + BWI76_RS12210 0.58 -1.4 2,555,139 - BWI76_RS12210 0.79 -1.1 2,555,159 + BWI76_RS12210 0.87 -0.5 2,555,167 - +0.7 2,555,170 + +0.3 2,555,170 + -1.4 2,555,178 - -0.8 2,555,178 - -0.8 2,555,178 - +0.5 2,555,192 - -0.0 2,555,242 + +0.4 2,555,270 - BWI76_RS12215 0.15 -0.0 2,555,312 + BWI76_RS12215 0.48 +0.0 2,555,312 + BWI76_RS12215 0.48 -0.2 2,555,320 - BWI76_RS12215 0.55 -0.5 2,555,329 - BWI76_RS12215 0.62 +0.6 2,555,342 - BWI76_RS12215 0.72 -0.1 2,555,353 - BWI76_RS12215 0.81 -1.3 2,555,354 + BWI76_RS12215 0.82 +1.4 2,555,362 - BWI76_RS12215 0.88 -1.7 2,555,372 - +0.8 2,555,381 + +0.2 2,555,387 - +0.6 2,555,416 - -0.4 2,555,421 - -1.7 2,555,446 - -0.5 2,555,470 - +0.3 2,555,511 - +0.8 2,555,548 + +0.5 2,555,548 + +0.5 2,555,554 - -1.5 2,555,556 - -1.2 2,555,561 + -0.9 2,555,591 + -0.2 2,555,612 - +3.4 2,555,628 + -0.0 2,555,641 + +0.4 2,555,652 + -1.0 2,555,694 + +1.3 2,555,694 + +0.8 2,555,797 + BWI76_RS12220 0.13 -1.4 2,555,808 - BWI76_RS12220 0.17 -1.4 2,555,814 + BWI76_RS12220 0.20 -0.8 2,555,814 + BWI76_RS12220 0.20 +0.1 2,555,817 - BWI76_RS12220 0.21 +0.1 2,555,817 - BWI76_RS12220 0.21 -0.3 2,555,819 + BWI76_RS12220 0.22 +2.2 2,555,822 - BWI76_RS12220 0.23 -0.8 2,555,840 + BWI76_RS12220 0.31 +1.1 2,555,848 - BWI76_RS12220 0.34 -0.3 2,555,848 - BWI76_RS12220 0.34 +0.9 2,555,884 + BWI76_RS12220 0.49 +0.4 2,555,884 + BWI76_RS12220 0.49 -1.0 2,555,892 - BWI76_RS12220 0.53 -0.3 2,555,892 - BWI76_RS12220 0.53 -1.0 2,555,892 - BWI76_RS12220 0.53 +1.2 2,555,908 - BWI76_RS12220 0.59 -1.0 2,555,951 + BWI76_RS12220 0.77 -0.7 2,555,951 + BWI76_RS12220 0.77 -0.5 2,555,951 + BWI76_RS12220 0.77 +0.6 2,555,951 + BWI76_RS12220 0.77 +0.7 2,555,961 - BWI76_RS12220 0.81 -1.0 2,556,091 + -1.7 2,556,091 + +3.9 2,556,091 + -0.1 2,556,091 + -1.4 2,556,091 + -0.8 2,556,091 + -0.6 2,556,099 + +0.1 2,556,099 - +0.8 2,556,100 + +0.5 2,556,101 + -0.7 2,556,101 + -1.2 2,556,105 + -1.3 2,556,114 + +1.9 2,556,114 + -0.4 2,556,114 + +0.5 2,556,122 - -0.0 2,556,137 + +1.8 2,556,140 - -0.2
Or see this region's nucleotide sequence