Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11165 and BWI76_RS11170 are separated by 36 nucleotides BWI76_RS11170 and BWI76_RS11175 are separated by 233 nucleotides BWI76_RS11175 and BWI76_RS11180 are separated by 225 nucleotides BWI76_RS11180 and BWI76_RS11185 are separated by 56 nucleotides
BWI76_RS11165: BWI76_RS11165 - beta-N-acetylhexosaminidase, at 2,352,837 to 2,353,859
_RS11165
BWI76_RS11170: BWI76_RS11170 - hypothetical protein, at 2,353,896 to 2,354,438
_RS11170
BWI76_RS11175: BWI76_RS11175 - NADH dehydrogenase, at 2,354,672 to 2,355,976
_RS11175
BWI76_RS11180: BWI76_RS11180 - hypothetical protein, at 2,356,202 to 2,356,741
_RS11180
BWI76_RS11185: BWI76_RS11185 - ligand-gated channel protein, at 2,356,798 to 2,358,975
_RS11185
Position (kb)
2354
2355
2356 Strain fitness (log2 ratio)
-1
0
1 at 2353.799 kb on - strand at 2353.821 kb on - strand at 2354.214 kb on - strand, within BWI76_RS11170 at 2354.721 kb on + strand at 2356.272 kb on + strand, within BWI76_RS11180 at 2356.313 kb on + strand, within BWI76_RS11180 at 2356.561 kb on + strand, within BWI76_RS11180 at 2356.707 kb on + strand at 2356.867 kb on + strand at 2356.880 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 2,353,799 - -0.1 2,353,821 - -0.5 2,354,214 - BWI76_RS11170 0.59 +1.2 2,354,721 + -1.6 2,356,272 + BWI76_RS11180 0.13 -0.0 2,356,313 + BWI76_RS11180 0.21 -0.6 2,356,561 + BWI76_RS11180 0.66 +0.0 2,356,707 + -0.1 2,356,867 + -0.7 2,356,880 - -0.4
Or see this region's nucleotide sequence