Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11155 and BWI76_RS11160 overlap by 20 nucleotides BWI76_RS11160 and BWI76_RS11165 are separated by 19 nucleotides BWI76_RS11165 and BWI76_RS11170 are separated by 36 nucleotides BWI76_RS11170 and BWI76_RS11175 are separated by 233 nucleotides
BWI76_RS11155: BWI76_RS11155 - penicillin-binding protein activator LpoB, at 2,351,383 to 2,352,018
_RS11155
BWI76_RS11160: BWI76_RS11160 - thiamine kinase, at 2,351,999 to 2,352,817
_RS11160
BWI76_RS11165: BWI76_RS11165 - beta-N-acetylhexosaminidase, at 2,352,837 to 2,353,859
_RS11165
BWI76_RS11170: BWI76_RS11170 - hypothetical protein, at 2,353,896 to 2,354,438
_RS11170
BWI76_RS11175: BWI76_RS11175 - NADH dehydrogenase, at 2,354,672 to 2,355,976
_RS11175
Position (kb)
2352
2353
2354 Strain fitness (log2 ratio)
-2
-1
0
1 at 2351.881 kb on - strand, within BWI76_RS11155 at 2352.007 kb on - strand at 2352.066 kb on - strand at 2352.951 kb on - strand at 2353.075 kb on - strand, within BWI76_RS11165 at 2353.102 kb on + strand, within BWI76_RS11165 at 2353.102 kb on + strand, within BWI76_RS11165 at 2353.110 kb on - strand, within BWI76_RS11165 at 2353.206 kb on + strand, within BWI76_RS11165 at 2353.214 kb on - strand, within BWI76_RS11165 at 2353.799 kb on - strand at 2353.821 kb on - strand at 2354.214 kb on - strand, within BWI76_RS11170 at 2354.721 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 2,351,881 - BWI76_RS11155 0.78 -2.6 2,352,007 - -0.1 2,352,066 - -0.1 2,352,951 - +1.2 2,353,075 - BWI76_RS11165 0.23 -1.5 2,353,102 + BWI76_RS11165 0.26 -0.2 2,353,102 + BWI76_RS11165 0.26 -0.4 2,353,110 - BWI76_RS11165 0.27 +0.3 2,353,206 + BWI76_RS11165 0.36 -0.6 2,353,214 - BWI76_RS11165 0.37 +1.2 2,353,799 - -0.1 2,353,821 - -0.5 2,354,214 - BWI76_RS11170 0.59 +1.2 2,354,721 + -1.6
Or see this region's nucleotide sequence