Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS09725

Experiment: Parafilmed volatile agar plate with no fungus

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS09715 and BWI76_RS09720 are separated by 17 nucleotidesBWI76_RS09720 and BWI76_RS09725 are separated by 122 nucleotidesBWI76_RS09725 and BWI76_RS09730 are separated by 53 nucleotidesBWI76_RS09730 and BWI76_RS09735 are separated by 203 nucleotides BWI76_RS09715: BWI76_RS09715 - hypothetical protein, at 2,036,291 to 2,037,556 _RS09715 BWI76_RS09720: BWI76_RS09720 - PTS ascorbate transporter subunit IIB, at 2,037,574 to 2,037,849 _RS09720 BWI76_RS09725: BWI76_RS09725 - MurR/RpiR family transcriptional regulator, at 2,037,972 to 2,038,829 _RS09725 BWI76_RS09730: BWI76_RS09730 - pyruvate formate lyase 1-activating protein, at 2,038,883 to 2,039,623 _RS09730 BWI76_RS09735: BWI76_RS09735 - formate acetyltransferase, at 2,039,827 to 2,042,109 _RS09735 Position (kb) 2037 2038 2039Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2036.998 kb on - strand, within BWI76_RS09715at 2037.174 kb on + strand, within BWI76_RS09715at 2037.228 kb on - strand, within BWI76_RS09715at 2037.605 kb on + strand, within BWI76_RS09720at 2037.623 kb on - strand, within BWI76_RS09720at 2037.628 kb on - strand, within BWI76_RS09720at 2037.728 kb on + strand, within BWI76_RS09720at 2037.855 kb on + strandat 2038.181 kb on + strand, within BWI76_RS09725at 2038.544 kb on + strand, within BWI76_RS09725at 2038.585 kb on + strand, within BWI76_RS09725at 2038.785 kb on + strandat 2038.881 kb on + strandat 2039.113 kb on - strand, within BWI76_RS09730at 2039.385 kb on - strand, within BWI76_RS09730at 2039.572 kb on - strandat 2039.746 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus
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2,036,998 - BWI76_RS09715 0.56 -1.1
2,037,174 + BWI76_RS09715 0.70 +0.2
2,037,228 - BWI76_RS09715 0.74 -0.7
2,037,605 + BWI76_RS09720 0.11 +0.4
2,037,623 - BWI76_RS09720 0.18 -0.7
2,037,628 - BWI76_RS09720 0.20 -0.1
2,037,728 + BWI76_RS09720 0.56 -0.4
2,037,855 + -2.0
2,038,181 + BWI76_RS09725 0.24 -0.1
2,038,544 + BWI76_RS09725 0.67 +0.4
2,038,585 + BWI76_RS09725 0.71 +3.5
2,038,785 + -0.0
2,038,881 + +1.9
2,039,113 - BWI76_RS09730 0.31 -0.6
2,039,385 - BWI76_RS09730 0.68 -0.0
2,039,572 - -1.6
2,039,746 - -0.6

Or see this region's nucleotide sequence