Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09430 and BWI76_RS09435 are separated by 6 nucleotides BWI76_RS09435 and BWI76_RS09440 are separated by 204 nucleotides BWI76_RS09440 and BWI76_RS09445 overlap by 17 nucleotides BWI76_RS09445 and BWI76_RS09450 are separated by 103 nucleotides
BWI76_RS09430: BWI76_RS09430 - hypothetical protein, at 1,976,797 to 1,977,180
_RS09430
BWI76_RS09435: BWI76_RS09435 - glutaredoxin, GrxA family, at 1,977,187 to 1,977,450
_RS09435
BWI76_RS09440: BWI76_RS09440 - hypothetical protein, at 1,977,655 to 1,977,945
_RS09440
BWI76_RS09445: BWI76_RS09445 - NADPH-dependent oxidoreductase, at 1,977,929 to 1,978,651
_RS09445
BWI76_RS09450: BWI76_RS09450 - 30S ribosomal protein S6--L-glutamate ligase, at 1,978,755 to 1,979,657
_RS09450
Position (kb) 1977
1978
1979 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1977.094 kb on + strand, within BWI76_RS09430 at 1977.094 kb on + strand, within BWI76_RS09430 at 1977.096 kb on + strand, within BWI76_RS09430 at 1977.096 kb on + strand, within BWI76_RS09430 at 1977.102 kb on - strand, within BWI76_RS09430 at 1977.162 kb on + strand at 1977.460 kb on + strand at 1977.468 kb on - strand at 1977.468 kb on - strand at 1977.729 kb on - strand, within BWI76_RS09440 at 1977.893 kb on - strand, within BWI76_RS09440 at 1978.172 kb on + strand, within BWI76_RS09445 at 1978.232 kb on + strand, within BWI76_RS09445 at 1978.300 kb on + strand, within BWI76_RS09445 at 1978.633 kb on + strand at 1978.728 kb on + strand at 1978.787 kb on + strand at 1979.000 kb on + strand, within BWI76_RS09450 at 1979.534 kb on + strand, within BWI76_RS09450
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 1,977,094 + BWI76_RS09430 0.77 +0.5 1,977,094 + BWI76_RS09430 0.77 +0.0 1,977,096 + BWI76_RS09430 0.78 +0.4 1,977,096 + BWI76_RS09430 0.78 -0.8 1,977,102 - BWI76_RS09430 0.79 +0.2 1,977,162 + +2.1 1,977,460 + -0.8 1,977,468 - -2.2 1,977,468 - -0.7 1,977,729 - BWI76_RS09440 0.25 +0.1 1,977,893 - BWI76_RS09440 0.82 +0.1 1,978,172 + BWI76_RS09445 0.34 -1.6 1,978,232 + BWI76_RS09445 0.42 -1.1 1,978,300 + BWI76_RS09445 0.51 -1.4 1,978,633 + +0.3 1,978,728 + -0.9 1,978,787 + +0.2 1,979,000 + BWI76_RS09450 0.27 +0.5 1,979,534 + BWI76_RS09450 0.86 +0.7
Or see this region's nucleotide sequence