Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09075 and BWI76_RS09080 are separated by 152 nucleotides BWI76_RS09080 and BWI76_RS09085 overlap by 1 nucleotides BWI76_RS09085 and BWI76_RS09090 are separated by 177 nucleotides
BWI76_RS09075: BWI76_RS09075 - pyruvate formate lyase-activating protein, at 1,916,043 to 1,916,942
_RS09075
BWI76_RS09080: BWI76_RS09080 - molybdopterin-synthase adenylyltransferase MoeB, at 1,917,095 to 1,917,850
_RS09080
BWI76_RS09085: BWI76_RS09085 - molybdopterin molybdenumtransferase MoeA, at 1,917,850 to 1,919,085
_RS09085
BWI76_RS09090: BWI76_RS09090 - beta-aspartyl-peptidase, at 1,919,263 to 1,920,204
_RS09090
Position (kb) 1917
1918
1919
1920 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1917.117 kb on + strand at 1917.343 kb on - strand, within BWI76_RS09080 at 1917.670 kb on + strand, within BWI76_RS09080 at 1917.818 kb on - strand at 1917.838 kb on + strand at 1917.865 kb on - strand at 1918.082 kb on - strand, within BWI76_RS09085 at 1918.231 kb on + strand, within BWI76_RS09085 at 1918.347 kb on - strand, within BWI76_RS09085 at 1918.418 kb on + strand, within BWI76_RS09085 at 1918.435 kb on + strand, within BWI76_RS09085 at 1918.585 kb on - strand, within BWI76_RS09085 at 1918.846 kb on + strand, within BWI76_RS09085 at 1919.054 kb on - strand at 1919.139 kb on - strand at 1919.319 kb on + strand at 1919.343 kb on + strand at 1919.348 kb on - strand at 1919.353 kb on - strand at 1919.403 kb on - strand, within BWI76_RS09090 at 1919.416 kb on - strand, within BWI76_RS09090 at 1919.476 kb on - strand, within BWI76_RS09090 at 1919.975 kb on - strand, within BWI76_RS09090 at 1920.075 kb on + strand, within BWI76_RS09090
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 1,917,117 + +1.8 1,917,343 - BWI76_RS09080 0.33 +1.9 1,917,670 + BWI76_RS09080 0.76 +0.6 1,917,818 - -1.1 1,917,838 + +2.0 1,917,865 - -1.1 1,918,082 - BWI76_RS09085 0.19 +1.3 1,918,231 + BWI76_RS09085 0.31 +1.0 1,918,347 - BWI76_RS09085 0.40 +1.1 1,918,418 + BWI76_RS09085 0.46 +1.9 1,918,435 + BWI76_RS09085 0.47 -0.1 1,918,585 - BWI76_RS09085 0.59 +1.5 1,918,846 + BWI76_RS09085 0.81 +1.5 1,919,054 - +1.3 1,919,139 - -0.9 1,919,319 + -2.2 1,919,343 + -1.3 1,919,348 - -0.0 1,919,353 - -0.1 1,919,403 - BWI76_RS09090 0.15 -0.3 1,919,416 - BWI76_RS09090 0.16 -1.2 1,919,476 - BWI76_RS09090 0.23 +0.4 1,919,975 - BWI76_RS09090 0.76 -0.6 1,920,075 + BWI76_RS09090 0.86 -0.1
Or see this region's nucleotide sequence