Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08820 and BWI76_RS08825 are separated by 3 nucleotides BWI76_RS08825 and BWI76_RS08830 overlap by 8 nucleotides BWI76_RS08830 and BWI76_RS08835 are separated by 1 nucleotides BWI76_RS08835 and BWI76_RS08840 are separated by 44 nucleotides BWI76_RS08840 and BWI76_RS08845 are separated by 190 nucleotides
BWI76_RS08820: BWI76_RS08820 - molybdenum cofactor biosynthesis protein, at 1,859,520 to 1,860,032
_RS08820
BWI76_RS08825: BWI76_RS08825 - cyclic pyranopterin monophosphate synthase MoaC, at 1,860,036 to 1,860,524
_RS08825
BWI76_RS08830: BWI76_RS08830 - molybdopterin synthase sulfur carrier subunit, at 1,860,517 to 1,860,765
_RS08830
BWI76_RS08835: BWI76_RS08835 - molybdopterin guanine dinucleotide biosynthesis protein MoaE, at 1,860,767 to 1,861,219
_RS08835
BWI76_RS08840: BWI76_RS08840 - hypothetical protein, at 1,861,264 to 1,861,968
_RS08840
BWI76_RS08845: BWI76_RS08845 - hypothetical protein, at 1,862,159 to 1,863,127
_RS08845
Position (kb) 1860
1861
1862 Strain fitness (log2 ratio) -1
0
1
2 at 1859.821 kb on - strand, within BWI76_RS08820 at 1859.866 kb on - strand, within BWI76_RS08820 at 1859.965 kb on + strand, within BWI76_RS08820 at 1860.154 kb on - strand, within BWI76_RS08825 at 1860.268 kb on - strand, within BWI76_RS08825 at 1860.425 kb on - strand, within BWI76_RS08825 at 1860.563 kb on - strand, within BWI76_RS08830 at 1860.768 kb on - strand at 1860.825 kb on - strand, within BWI76_RS08835 at 1860.886 kb on + strand, within BWI76_RS08835 at 1860.926 kb on - strand, within BWI76_RS08835 at 1860.928 kb on - strand, within BWI76_RS08835 at 1861.135 kb on + strand, within BWI76_RS08835 at 1861.327 kb on + strand at 1861.355 kb on + strand, within BWI76_RS08840 at 1861.769 kb on - strand, within BWI76_RS08840 at 1862.127 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 1,859,821 - BWI76_RS08820 0.59 +1.5 1,859,866 - BWI76_RS08820 0.67 +2.0 1,859,965 + BWI76_RS08820 0.87 -0.3 1,860,154 - BWI76_RS08825 0.24 +1.4 1,860,268 - BWI76_RS08825 0.47 +1.6 1,860,425 - BWI76_RS08825 0.80 +0.4 1,860,563 - BWI76_RS08830 0.18 +2.6 1,860,768 - +1.8 1,860,825 - BWI76_RS08835 0.13 +1.3 1,860,886 + BWI76_RS08835 0.26 +1.6 1,860,926 - BWI76_RS08835 0.35 +1.6 1,860,928 - BWI76_RS08835 0.36 -1.6 1,861,135 + BWI76_RS08835 0.81 +1.3 1,861,327 + +2.1 1,861,355 + BWI76_RS08840 0.13 -0.1 1,861,769 - BWI76_RS08840 0.72 -0.5 1,862,127 + +1.1
Or see this region's nucleotide sequence