Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08805 and BWI76_RS08810 are separated by 28 nucleotides BWI76_RS08810 and BWI76_RS08815 are separated by 111 nucleotides BWI76_RS08815 and BWI76_RS08820 are separated by 11 nucleotides BWI76_RS08820 and BWI76_RS08825 are separated by 3 nucleotides
BWI76_RS08805: BWI76_RS08805 - gluconeogenesis factor, at 1,857,269 to 1,858,174
_RS08805
BWI76_RS08810: BWI76_RS08810 - hypothetical protein, at 1,858,203 to 1,858,397
_RS08810
BWI76_RS08815: BWI76_RS08815 - cyclic pyranopterin phosphate synthase, at 1,858,509 to 1,859,508
_RS08815
BWI76_RS08820: BWI76_RS08820 - molybdenum cofactor biosynthesis protein, at 1,859,520 to 1,860,032
_RS08820
BWI76_RS08825: BWI76_RS08825 - cyclic pyranopterin monophosphate synthase MoaC, at 1,860,036 to 1,860,524
_RS08825
Position (kb)
1858
1859
1860 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1857.550 kb on - strand, within BWI76_RS08805 at 1857.725 kb on + strand, within BWI76_RS08805 at 1857.978 kb on - strand, within BWI76_RS08805 at 1858.072 kb on + strand, within BWI76_RS08805 at 1858.214 kb on + strand at 1858.397 kb on + strand at 1858.397 kb on + strand at 1858.404 kb on - strand at 1858.505 kb on + strand at 1858.513 kb on + strand at 1858.833 kb on + strand, within BWI76_RS08815 at 1859.001 kb on - strand, within BWI76_RS08815 at 1859.011 kb on + strand, within BWI76_RS08815 at 1859.062 kb on + strand, within BWI76_RS08815 at 1859.252 kb on + strand, within BWI76_RS08815 at 1859.327 kb on + strand, within BWI76_RS08815 at 1859.536 kb on - strand at 1859.592 kb on + strand, within BWI76_RS08820 at 1859.821 kb on - strand, within BWI76_RS08820 at 1859.866 kb on - strand, within BWI76_RS08820 at 1859.965 kb on + strand, within BWI76_RS08820 at 1860.154 kb on - strand, within BWI76_RS08825 at 1860.268 kb on - strand, within BWI76_RS08825 at 1860.425 kb on - strand, within BWI76_RS08825
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 1,857,550 - BWI76_RS08805 0.31 +1.5 1,857,725 + BWI76_RS08805 0.50 +0.1 1,857,978 - BWI76_RS08805 0.78 -0.4 1,858,072 + BWI76_RS08805 0.89 -1.6 1,858,214 + -0.5 1,858,397 + +3.0 1,858,397 + -0.4 1,858,404 - +1.9 1,858,505 + +0.5 1,858,513 + +3.7 1,858,833 + BWI76_RS08815 0.32 +0.2 1,859,001 - BWI76_RS08815 0.49 +1.3 1,859,011 + BWI76_RS08815 0.50 +1.6 1,859,062 + BWI76_RS08815 0.55 +1.8 1,859,252 + BWI76_RS08815 0.74 +0.9 1,859,327 + BWI76_RS08815 0.82 +2.1 1,859,536 - +0.6 1,859,592 + BWI76_RS08820 0.14 +0.3 1,859,821 - BWI76_RS08820 0.59 +1.5 1,859,866 - BWI76_RS08820 0.67 +2.0 1,859,965 + BWI76_RS08820 0.87 -0.3 1,860,154 - BWI76_RS08825 0.24 +1.4 1,860,268 - BWI76_RS08825 0.47 +1.6 1,860,425 - BWI76_RS08825 0.80 +0.4
Or see this region's nucleotide sequence