Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07695 and BWI76_RS07700 overlap by 8 nucleotides BWI76_RS07700 and BWI76_RS07705 are separated by 64 nucleotides BWI76_RS07705 and BWI76_RS07710 are separated by 27 nucleotides BWI76_RS07710 and BWI76_RS07715 are separated by 10 nucleotides
BWI76_RS07695: BWI76_RS07695 - TonB-dependent siderophore receptor, at 1,629,842 to 1,632,091
_RS07695
BWI76_RS07700: BWI76_RS07700 - hypothetical protein, at 1,632,084 to 1,632,297
_RS07700
BWI76_RS07705: BWI76_RS07705 - enterochelin esterase, at 1,632,362 to 1,633,561
_RS07705
BWI76_RS07710: BWI76_RS07710 - invasin-like protein, at 1,633,589 to 1,633,792
_RS07710
BWI76_RS07715: BWI76_RS07715 - non-ribosomal peptide synthetase, at 1,633,803 to 1,637,690
_RS07715
Position (kb)
1632
1633
1634 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1631.539 kb on + strand, within BWI76_RS07695 at 1631.758 kb on + strand, within BWI76_RS07695 at 1631.842 kb on + strand, within BWI76_RS07695 at 1631.850 kb on - strand, within BWI76_RS07695 at 1632.194 kb on + strand, within BWI76_RS07700 at 1632.372 kb on - strand at 1632.427 kb on - strand at 1632.635 kb on - strand, within BWI76_RS07705 at 1632.875 kb on - strand, within BWI76_RS07705 at 1632.971 kb on + strand, within BWI76_RS07705 at 1633.541 kb on + strand at 1633.763 kb on + strand, within BWI76_RS07710 at 1633.763 kb on + strand, within BWI76_RS07710 at 1633.763 kb on + strand, within BWI76_RS07710 at 1633.897 kb on + strand at 1633.905 kb on - strand at 1633.972 kb on + strand at 1633.980 kb on - strand at 1634.061 kb on - strand at 1634.158 kb on + strand at 1634.397 kb on + strand, within BWI76_RS07715 at 1634.410 kb on - strand, within BWI76_RS07715 at 1634.558 kb on - strand, within BWI76_RS07715
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 1,631,539 + BWI76_RS07695 0.75 +0.6 1,631,758 + BWI76_RS07695 0.85 -0.5 1,631,842 + BWI76_RS07695 0.89 +0.6 1,631,850 - BWI76_RS07695 0.89 +0.5 1,632,194 + BWI76_RS07700 0.51 +0.2 1,632,372 - -0.4 1,632,427 - +0.2 1,632,635 - BWI76_RS07705 0.23 -2.4 1,632,875 - BWI76_RS07705 0.43 -4.0 1,632,971 + BWI76_RS07705 0.51 -0.0 1,633,541 + +0.8 1,633,763 + BWI76_RS07710 0.85 -1.7 1,633,763 + BWI76_RS07710 0.85 -1.1 1,633,763 + BWI76_RS07710 0.85 +0.2 1,633,897 + -1.2 1,633,905 - -0.1 1,633,972 + -0.6 1,633,980 - +0.0 1,634,061 - -0.7 1,634,158 + +1.0 1,634,397 + BWI76_RS07715 0.15 +0.4 1,634,410 - BWI76_RS07715 0.16 +3.2 1,634,558 - BWI76_RS07715 0.19 -0.7
Or see this region's nucleotide sequence