Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06530 and BWI76_RS06535 are separated by 183 nucleotides BWI76_RS06535 and BWI76_RS06540 are separated by 208 nucleotides BWI76_RS06540 and BWI76_RS06545 are separated by 213 nucleotides
BWI76_RS06530: BWI76_RS06530 - ATP-dependent Clp protease ATP-binding subunit ClpX, at 1,371,243 to 1,372,517
_RS06530
BWI76_RS06535: BWI76_RS06535 - endopeptidase La, at 1,372,701 to 1,375,055
_RS06535
BWI76_RS06540: BWI76_RS06540 - DNA-binding protein HU-beta, at 1,375,264 to 1,375,536
_RS06540
BWI76_RS06545: BWI76_RS06545 - peptidylprolyl isomerase, at 1,375,750 to 1,377,624
_RS06545
Position (kb)
1372
1373
1374
1375
1376 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 1371.705 kb on + strand, within BWI76_RS06530 at 1371.748 kb on - strand, within BWI76_RS06530 at 1372.140 kb on - strand, within BWI76_RS06530 at 1372.382 kb on + strand, within BWI76_RS06530 at 1372.590 kb on + strand at 1372.827 kb on + strand at 1372.968 kb on + strand, within BWI76_RS06535 at 1373.007 kb on - strand, within BWI76_RS06535 at 1373.007 kb on - strand, within BWI76_RS06535 at 1373.986 kb on + strand, within BWI76_RS06535 at 1374.121 kb on - strand, within BWI76_RS06535 at 1374.267 kb on + strand, within BWI76_RS06535 at 1374.486 kb on + strand, within BWI76_RS06535 at 1374.486 kb on + strand, within BWI76_RS06535 at 1374.890 kb on + strand at 1374.898 kb on - strand at 1375.143 kb on - strand at 1375.165 kb on + strand at 1375.166 kb on - strand at 1375.170 kb on + strand at 1375.173 kb on - strand at 1375.177 kb on + strand at 1375.199 kb on + strand at 1375.222 kb on + strand at 1375.425 kb on - strand, within BWI76_RS06540 at 1375.507 kb on + strand, within BWI76_RS06540 at 1375.636 kb on + strand at 1375.636 kb on + strand at 1375.836 kb on + strand at 1376.020 kb on + strand, within BWI76_RS06545
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 1,371,705 + BWI76_RS06530 0.36 +3.2 1,371,748 - BWI76_RS06530 0.40 +4.8 1,372,140 - BWI76_RS06530 0.70 +1.5 1,372,382 + BWI76_RS06530 0.89 +3.6 1,372,590 + -0.4 1,372,827 + -3.7 1,372,968 + BWI76_RS06535 0.11 -1.6 1,373,007 - BWI76_RS06535 0.13 -1.4 1,373,007 - BWI76_RS06535 0.13 -1.2 1,373,986 + BWI76_RS06535 0.55 -1.8 1,374,121 - BWI76_RS06535 0.60 -0.6 1,374,267 + BWI76_RS06535 0.66 -2.6 1,374,486 + BWI76_RS06535 0.76 -1.5 1,374,486 + BWI76_RS06535 0.76 +0.0 1,374,890 + -1.4 1,374,898 - -0.8 1,375,143 - -1.0 1,375,165 + +1.6 1,375,166 - -0.1 1,375,170 + -1.2 1,375,173 - -1.1 1,375,177 + +0.1 1,375,199 + +0.1 1,375,222 + -0.9 1,375,425 - BWI76_RS06540 0.59 -1.1 1,375,507 + BWI76_RS06540 0.89 -0.8 1,375,636 + -0.8 1,375,636 + +1.4 1,375,836 + -1.4 1,376,020 + BWI76_RS06545 0.14 -1.2
Or see this region's nucleotide sequence